y****6
发帖数: 196 | 1 最近用MDA-MB-231 cell 在裸鼠皮下生成肿瘤失败(s.c.injection on back, male
nude mice, 5 million cells in 100 ul DMEM w/o serum, no tumor after >6 weeks
)。同样条件SK-OV-3可以产生肿瘤。我印象中MDA-MB-231比SK-OV-3容易生成肿瘤。有
经验的请说说可能问题出在那里？不会因为MDA-MB-231在公鼠上不长吧。 | q*****n
发帖数: 331 | 2 Why not try female mice?
MDA-MB-231 is not a very aggressive model for breast cancer. Joan Massague'
group have developed some derivative cell lines, such as LM2, which grows
faster and metastasize better. here is the reference:
Nature. 2005 Jul 28;436(7050):518-24.
Genes that mediate breast cancer metastasis to lung.
Minn AJ, Gupta GP, Siegel PM, Bos PD, Shu W, Giri DD, Viale A, Olshen AB,
Gerald WL, Massagué J.
You can write and request LM2 cells and they will give for free. | A******y
发帖数: 2041 | 3 Use matrigel for consistency, and expect about 2 out 10 mice won't even
engraft. You also need about 10 million cells per injection. | y****6
发帖数: 196 | 4 Thanks a lot for the information. However I already made a stable cell line
from the original MDA-MB-231, which means I cannot use the derivatives. I
will try female mice, and inject more cells (as suggested by "ArtyArty").
MDA-MB-231 is not a very aggressive model for breast cancer. Joan Massague'
group have developed some derivative cell lines, such as LM2, which grows
faster and metastasize better. here is the reference:
Nature. 2005 Jul 28;436(7050):518-24.
Genes that mediate breast cancer metastasis to lung.
Minn AJ, Gupta GP, Siegel PM, Bos PD, Shu W, Giri DD, Viale A, Olshen AB,
Gerald WL, Massagué J. | m********8
发帖数: 314 | 5 Female nude rat modeling can be succeeded at 80% tumor rate about 3 weeks
post 6M cells in 0.2 ml saline were injected orthotopically. | D******9
发帖数: 2665 | 6 I used 2X105 231 cells, developed tumors in 4 weeks.There's something wrong
with your cells.Try to get a new vial from other lab. | y****6
发帖数: 196 | 7 Do you inject at the mammary gland? I do not know whether that makes
difference. Thanks.
I used 2X105 231 cells, developed tumors in 4 weeks.There's something wrong
with your cells.Try to get a new vial from other lab. | D******9
发帖数: 2665 | 8 no you don't have to. actually mouse and human breast structures are very
different. you can not mimic human breast environment in mouse. |
| D******9
发帖数: 2665 | 9 MDA231 is a triple-negative breast cancer cell, very aggressive. The size of
needle matters, do not use too narrow needle because it may hurt the cells.
| g****0
发帖数: 425 | 10 May I ask for the best ref for your statement? Thanks,
【在 D******9 的大作中提到】
: no you don't have to. actually mouse and human breast structures are very
: different. you can not mimic human breast environment in mouse.
| c*******t
发帖数: 962 | 11 你是稳定转染的细胞株？敲掉/加入了什么基因？没处理之前的细胞株长不长？
如果只做过一次不能确定不能成瘤。比方消化时间过久也可能。
这个细胞系应该很容易做。绝对不要等6个礼拜。
line
derivatives. I
"ArtyArty").
Massague'
grows
AB,
【在 y****6 的大作中提到】
: Thanks a lot for the information. However I already made a stable cell line
: from the original MDA-MB-231, which means I cannot use the derivatives. I
: will try female mice, and inject more cells (as suggested by "ArtyArty").
: MDA-MB-231 is not a very aggressive model for breast cancer. Joan Massague'
: group have developed some derivative cell lines, such as LM2, which grows
: faster and metastasize better. here is the reference:
: Nature. 2005 Jul 28;436(7050):518-24.
: Genes that mediate breast cancer metastasis to lung.
: Minn AJ, Gupta GP, Siegel PM, Bos PD, Shu W, Giri DD, Viale A, Olshen AB,
: Gerald WL, Massagué J.
| y****6
发帖数: 196 | 12 The parental cells do not grow tumors. So I believe something is wrong with
my system.
【在 c*******t 的大作中提到】
: 你是稳定转染的细胞株？敲掉/加入了什么基因？没处理之前的细胞株长不长？
: 如果只做过一次不能确定不能成瘤。比方消化时间过久也可能。
: 这个细胞系应该很容易做。绝对不要等6个礼拜。
:
: line
: derivatives. I
: "ArtyArty").
: Massague'
: grows
: AB,
| a*****g
发帖数: 543 | 13 assume you have done mycoplasma test?
with
【在 y****6 的大作中提到】
: The parental cells do not grow tumors. So I believe something is wrong with
: my system.
|
|
