W*T
发帖数: 359 | | M**a
发帖数: 4816 | 2 A long list. To save time, try google/web of knowledge,using "single cell
analysis".
【在 W*T 的大作中提到】
: 如何从plate挑单细胞？thanks
| i**o
发帖数: 76 | 3 http://www.nature.com/nmeth/journal/v8/n4s/full/nmeth.1557.html
The method for the isolation of individual cells can vary. Picking single
cells manually using a mouth pipette is the most straightforward option30,
41, although this can be time-consuming and technically challenging. Laser-
assisted microdissection or fluorescence-activated cell sorting can also be
used to isolate a specific subpopulation of cells based on cell-surface
markers or fluorescent reporters42, 43, 44, 45. Cells of higher plants with
cell walls are difficult to dissociate using enzymes, but isolation of
nuclei after homogenization of tissues might be effective | W*T
发帖数: 359 | 4 thanks
be
with
【在 i**o 的大作中提到】
: http://www.nature.com/nmeth/journal/v8/n4s/full/nmeth.1557.html
: The method for the isolation of individual cells can vary. Picking single
: cells manually using a mouth pipette is the most straightforward option30,
: 41, although this can be time-consuming and technically challenging. Laser-
: assisted microdissection or fluorescence-activated cell sorting can also be
: used to isolate a specific subpopulation of cells based on cell-surface
: markers or fluorescent reporters42, 43, 44, 45. Cells of higher plants with
: cell walls are difficult to dissociate using enzymes, but isolation of
: nuclei after homogenization of tissues might be effective
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