m******5
发帖数: 1383 | 1 贴上来确认一下…… 一个knock in要大半年才能拿到，怕有什么意外问题 |
g*********5
发帖数: 2533 | 2 expression ... none could guanrantee |
j*b
发帖数: 341 | 3 no problem.
check your mail.
【在 m******5 的大作中提到】
: 贴上来确认一下…… 一个knock in要大半年才能拿到，怕有什么意外问题
|
m******5
发帖数: 1383 | 4 我安全起见，在这个tag和我的蛋白间+了8个glycine做linker，因为问题不大吧？
【在 j*b 的大作中提到】
: no problem.
: check your mail.
|
j*b
发帖数: 341 | 5 Nature. 2010 Jan 21;463(7279):374-8.
Transcriptional role of cyclin D1 in development revealed by a genetic-
proteomic screen.
Abstract
Cyclin D1 belongs to the core cell cycle machinery, and it is frequently
overexpressed in human cancers. The full repertoire of cyclin D1 functions
in normal development and oncogenesis is unclear at present. Here we
developed Flag- and haemagglutinin-tagged cyclin D1 knock-in mouse strains
that allowed a high-throughput mass spectrometry approach to search for
cyclin D1-binding proteins in different mouse organs.
【在 m******5 的大作中提到】
: 我安全起见，在这个tag和我的蛋白间+了8个glycine做linker，因为问题不大吧？
|
m******5
发帖数: 1383 | 6 Thank you, I am at home………… Did they actually add any linker?
【在 j*b 的大作中提到】
: Nature. 2010 Jan 21;463(7279):374-8.
: Transcriptional role of cyclin D1 in development revealed by a genetic-
: proteomic screen.
: Abstract
: Cyclin D1 belongs to the core cell cycle machinery, and it is frequently
: overexpressed in human cancers. The full repertoire of cyclin D1 functions
: in normal development and oncogenesis is unclear at present. Here we
: developed Flag- and haemagglutinin-tagged cyclin D1 knock-in mouse strains
: that allowed a high-throughput mass spectrometry approach to search for
: cyclin D1-binding proteins in different mouse organs.
|
