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发帖数: 73 | 1 Hi all:
Recently I planning to do siRNA, but I have a detailed question.
I got siRNA from Dharmacon, and it came as dried pellet, which means I need
to dissolve it in some solutions. The instruction suggests resuspending the
dried pellet in RNase-free 1X siRNA buffer (60mM KCl, 6mM HEPES-KOH, pH7.5,
0.2mM MgCl2), or in RNase-free water for short-term storage.
It will take time to place another order only for those buffers. So I am
wondering how you guys do that. Can we make the buffer by ourselves, and
whether the home-made buffer toxic to the cells. And any suggestions about
my case?
Thanks very much!
PS: I can type Chinese on the computer. |
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