l*****i 发帖数: 1163 | 1 我现在做的分子方面的实验,构建载体原核表达,共构建了9个基因在PET 28C上,就3
个表达了,而且优化诱导后还是包涵体表达,请问怎么增加包涵体复性的机会?多谢了 | b******y 发帖数: 627 | 2 Don't recommend denature-renature from inclusion body. Not many successful
examples, especially for relatively large proteins. You will have better
chances if putting a good tag, (MBP, Sumo, GB1, .... even GST) in front of
your proteins. | l*****i 发帖数: 1163 | 3 非常感谢
【在 b******y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Don't recommend denature-renature from inclusion body. Not many successful : examples, especially for relatively large proteins. You will have better : chances if putting a good tag, (MBP, Sumo, GB1, .... even GST) in front of : your proteins.
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