l*****i
发帖数: 1163 | 1 我现在做的分子方面的实验，构建载体原核表达，共构建了9个基因在PET 28C上，就3
个表达了，而且优化诱导后还是包涵体表达，请问怎么增加包涵体复性的机会？多谢了 | b******y
发帖数: 627 | 2 Don't recommend denature-renature from inclusion body. Not many successful
examples, especially for relatively large proteins. You will have better
chances if putting a good tag, (MBP, Sumo, GB1, .... even GST) in front of
your proteins. | l*****i
发帖数: 1163 | 3 非常感谢
【在 b******y 的大作中提到】
: Don't recommend denature-renature from inclusion body. Not many successful
: examples, especially for relatively large proteins. You will have better
: chances if putting a good tag, (MBP, Sumo, GB1, .... even GST) in front of
: your proteins.
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