M*******C 发帖数: 183 | 4 我看了一下网站,兼容的试剂名单里没有SDS,你用它来做SDS的样品好用吗?
你说的蛋白沉淀下来再测是什么意思?就是裂解-离心-取上清?
比如我的细胞,我加了含SDS的去裂解,为了核蛋白,这样lysate很粘,我就去超声,
等离心后经常看不见沉淀,直接就拿去用了。
The RC DC protein assay is compatible with the following reagents and
buffers in addition to those compatible with the DC protein assay:
CHAPS 2% ReadyPrep sequential extraction reagents 2 and 3
DTT, 350 mM Sodium hydroxide, 2.5 M
EDTA, 0.1 M TBP, 2 mM
Imidazole, 0.5 M Tris, pH 8.4, 0.5 M
Laemmli buffer with 5% β-mercaptoethanol Triton X-100, 2%
β-Mercaptoethanol, 10% Tween 20, 2%
【在 s******s 的大作中提到】 : 蛋白沉淀下来再测就行了。biorad RC-DC
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