s****m
发帖数: 472 | 1 Hello Everyone,
Anybody studied cell cycle here? Is there any assays or markers to
distinguish G2 phase from M phase easily?
Thank you so much! |
m******5
发帖数: 1383 | 2 phorshpor Histon 3 is a common marker for mitosis phase
you can do a westernblot to find out
IHC also works |
b*******0
发帖数: 48 | 3 you can directly tell if the cell is in M phase by looking at the chromosome
arrangement. Cells in M phase tend to have no nuclear membrane and
condensed chromsomes are ready to be aligned at the plate.
A good marker for cells from last S to G2 phase is CEP-F antibody.
【在 s****m 的大作中提到】
: Hello Everyone,
: Anybody studied cell cycle here? Is there any assays or markers to
: distinguish G2 phase from M phase easily?
: Thank you so much!
|
s****m
发帖数: 472 | 4 thanks, I just check the abcam, where it has the human Anti-CENPF antibody,
is there any mouse or goat anti-CENPF antibody?
Thanks again
chromosome
【在 b*******0 的大作中提到】
: you can directly tell if the cell is in M phase by looking at the chromosome
: arrangement. Cells in M phase tend to have no nuclear membrane and
: condensed chromsomes are ready to be aligned at the plate.
: A good marker for cells from last S to G2 phase is CEP-F antibody.
|
