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Biology版 - how to avoid cross contamination of homogenizer in RNA iso
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1 (共1页)
m*****0
发帖数: 72
1
i need to isolate RNA from many different mouse tissues with homogenizer.
between samples, how to clean the homogenizer tips?
i roughly remember wash in 0.1N NaOH and then water, is that right?
or any suggestion if i stick to this metal homogenizer (can't change to
plasic tips)?
Thanks
l**********1
发帖数: 5204
2
LZ
pls refer below english protocol forum their replied:
Dodger on Tue Nov 23 00: 2010 said:
It might be a little late to post an answer to this thread due to it's age,
but since it's pinned...
We usually prepare 4x 50 mL tubes. Fill three with 40 mL DEPC treated water,
and one with 100% ethanol.
One of the DEPC treated water tubes gets 5-10 squirts of RNase Zap.
Between each sample, the homogenizer is dipped into 100% ethanol, RNase Zap
water, and 2x DEPC treated water in turn.
Each time turning the homogenizer on for about 15-30s.
This is fairly quick and seems to work well for us.
I do exactly like that. The difference is that a fill one tube ( 2ml) with
100% ethanol, one with treated water and RNAzap squirts and another one just
with treated water. I prepare these three tubes for each sample. So, after
the homogenizing I wash the tip of the homogenizer on these tubes in that
sequence. The next sample I change tubes and use new ones. My concern is
avoid cross-contamination. Like they said before, the tissue is full of
RNAse!
-Lilip-
//www.protocol-online.org/biology-forums-2/posts/11762more1.html

【在 m*****0 的大作中提到】
: i need to isolate RNA from many different mouse tissues with homogenizer.
: between samples, how to clean the homogenizer tips?
: i roughly remember wash in 0.1N NaOH and then water, is that right?
: or any suggestion if i stick to this metal homogenizer (can't change to
: plasic tips)?
: Thanks

1 (共1页)
进入Biology版参与讨论
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Re: Who knows about RNase H? --- ask for help!70%酒精的配制
有人用rna later么请教trizol提tissue RNA
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请问有没有从老鼠组织提过蛋白的? THXanybody has experience in zebrafish insitu hybridization?
Re: 问题:关于RNase裂解yeast的仪器
Re: RNA extraction做老鼠的xdjm们你们如何homogenize心脏的?
技术问题:DNase-free, RNase-free water?Re: 厚着脸皮问一句:1 OD到底对应多大浓度的单链DNA溶液?
RNA原位杂交问题请教问问关于RNA提取 260 230 比值太低问题
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