m******p 发帖数: 67 | 1 i have a sample, either total RNA or cDNA. anyone has a method to determine
whether it is RNA or cDNA?
Thanks |
s******y 发帖数: 28562 | 2 If you know the sequence you can use a pair of primers and the taq PCR
polymerase. The taq polymerase does not use RNA as template in an usual PCR
condition.
determine
【在 m******p 的大作中提到】![](/moin_static193/solenoid/img/up.png) : i have a sample, either total RNA or cDNA. anyone has a method to determine : whether it is RNA or cDNA? : Thanks
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m**********n 发帖数: 299 | 3 Use NanoDrop.
A260/A280, A260/A230;
See the reference for details.
http://batzerlab.lsu.edu/genomics/documentation/3130_NanoDrop_t |
m**********n 发帖数: 299 | 4 The simplest method is the best method.
PCR
【在 s******y 的大作中提到】![](/moin_static193/solenoid/img/up.png) : If you know the sequence you can use a pair of primers and the taq PCR : polymerase. The taq polymerase does not use RNA as template in an usual PCR : condition. : : determine
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s******y 发帖数: 28562 | 5 看了以下连接里面的内容,好像是说230的吸收度和buffer 有关系?
如果楼主不知道buffer 是什么的话恐怕很难办吧?
【在 m**********n 的大作中提到】![](/moin_static193/solenoid/img/up.png) : Use NanoDrop. : A260/A280, A260/A230; : See the reference for details. : http://batzerlab.lsu.edu/genomics/documentation/3130_NanoDrop_t
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m******p 发帖数: 67 | 6 smart methods. pcr and od. thanks. |
s*******e 发帖数: 1010 | 7 add DNAase/RNAase and run a gel?
What if you have a mixed sample? |
s******y 发帖数: 28562 | 8 这个也可以,如果样品比较多的话。
【在 s*******e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : add DNAase/RNAase and run a gel? : What if you have a mixed sample?
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m******p 发帖数: 67 | 9 wa.. another smart idea. |