E**********y
发帖数: 991 | | s****l
发帖数: 395 | 2 这玩意儿不是测量cell survival最直接的证据吗
【在 E**********y 的大作中提到】
: 这比一般什么XTT复杂很多，有什么优势？
| E**********y
发帖数: 991 | | A******y
发帖数: 2041 | 4 The differences is not at the detection method. The cells grow differently
in cell culture (liquid) verses colongenic method (semi-solid). I can give
you a perfect example which is not reported anywhere. HDAC inhibitor, SAHA,
inhibits leukemia cell growth in cell culture (many papers). However, it
is useless against leukemia growth in colongenic assay.
【在 E**********y 的大作中提到】
: XTT我觉得也很直接呀
| E**********y
发帖数: 991 | 5 is that why clonogenic survival assay becomes a standard assay to assess
therapeutic agents?
differently
give
SAHA,
【在 A******y 的大作中提到】
: The differences is not at the detection method. The cells grow differently
: in cell culture (liquid) verses colongenic method (semi-solid). I can give
: you a perfect example which is not reported anywhere. HDAC inhibitor, SAHA,
: inhibits leukemia cell growth in cell culture (many papers). However, it
: is useless against leukemia growth in colongenic assay.
| A******y
发帖数: 2041 | 6 Yes, you need it before in-vivo.
【在 E**********y 的大作中提到】
: is that why clonogenic survival assay becomes a standard assay to assess
: therapeutic agents?
:
: differently
: give
: SAHA,
|
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