x*******u 发帖数: 78 | 1 如题,请各位大虾赐教。代谢组学的扫盲的review之类的,万分感谢 |
b*******g 发帖数: 1309 | 2 check masspec.scripps.edu
【在 x*******u 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 如题,请各位大虾赐教。代谢组学的扫盲的review之类的,万分感谢
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s******a 发帖数: 252 | 3 Patti, G.J., Yanes, O. & Siuzdak, G. Metabolomics: the apogee of the omics
trilogy. Nature Reviews Molecular Cell Biology 13, 263-269(2012). |
h**********r 发帖数: 671 | 4 实验室买了这套仪器(Agilent 6530-Accurate-Mass Q-TOF LC/MS and Agilent 1290
UHPLC, Agilent 3243 GC MSD, and Aligent 4240 HPLC-Chip Cube),在没人指导没
人会用的情况下,读些啥可以尽快折腾折腾点有用的数据出来? |
y*****s 发帖数: 1047 | 5 http://metabolomics.chem.agilent.com/
1290
【在 h**********r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 实验室买了这套仪器(Agilent 6530-Accurate-Mass Q-TOF LC/MS and Agilent 1290 : UHPLC, Agilent 3243 GC MSD, and Aligent 4240 HPLC-Chip Cube),在没人指导没 : 人会用的情况下,读些啥可以尽快折腾折腾点有用的数据出来?
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s******a 发帖数: 252 | 6 Can you guys lend me for a few months? Our Orbi is down again :-(
Kidding aside, I like the idea in this paper -
Wu, Manhong, et al. "Identification of drug targets by chemogenomic and
metabolomic profiling in yeast." Pharmacogenetics and genomics 22.12 (2012):
877-886.
One can do untargeted MS and scale it up. I can help with computational work
if you get data.
1290
【在 h**********r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 实验室买了这套仪器(Agilent 6530-Accurate-Mass Q-TOF LC/MS and Agilent 1290 : UHPLC, Agilent 3243 GC MSD, and Aligent 4240 HPLC-Chip Cube),在没人指导没 : 人会用的情况下,读些啥可以尽快折腾折腾点有用的数据出来?
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h**********r 发帖数: 671 | 7 问题是弄了这个玩意儿不会用,而且把钱花光了。因为没钱,培训过的人也被fire了。
机器吃人啊~
我比较喜欢这类型的paper:
α-ketoglutarate coordinates carbon and nitrogen utilization via enzyme I
inhibition
Christopher D Doucette, David J Schwab, Ned S Wingreen & Joshua D Rabinowitz
Nature Chemical Biology 7, 894–901 (2011)
实验很多组能做,模型也不复杂。story还挺漂亮的。
另外,Rethinking glycolysis: on the biochemical logic of metabolic pathways
Nature Chemical Biology 8, 509–517 (2012)
Arren Bar-Even, Avi Flamholz, Elad Noor & Ron Milo
话说Weizmann真不缺牛人。
有data了一定找你!!! |
K******S 发帖数: 10109 | 8 manual books, try to get instrument up and running anything. then try to run
your real world samples.
1290
【在 h**********r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 实验室买了这套仪器(Agilent 6530-Accurate-Mass Q-TOF LC/MS and Agilent 1290 : UHPLC, Agilent 3243 GC MSD, and Aligent 4240 HPLC-Chip Cube),在没人指导没 : 人会用的情况下,读些啥可以尽快折腾折腾点有用的数据出来?
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b*******g 发帖数: 1309 | 9 read the instrument manual
Agilent instrument is very easy to use。
and read some protocol paper from nature protocols
1290
【在 h**********r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 实验室买了这套仪器(Agilent 6530-Accurate-Mass Q-TOF LC/MS and Agilent 1290 : UHPLC, Agilent 3243 GC MSD, and Aligent 4240 HPLC-Chip Cube),在没人指导没 : 人会用的情况下,读些啥可以尽快折腾折腾点有用的数据出来?
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