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Biology版 - anyone knows a way to irreversibly kill all kinases without denaturing other proteins?
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相关话题的讨论汇总
话题: kinases话题: denaturing
进入Biology版参与讨论
1 (共1页)
l****y
发帖数: 398
1
If it works in cell lysate, that should be good enough. Thanks
H****N
发帖数: 997
2
deplet the ATP
l****y
发帖数: 398
3
but the kinases are still active. i want them all dead.

【在 H****N 的大作中提到】
: deplet the ATP
1 (共1页)
进入Biology版参与讨论
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怎样设计小实验来确定一个基因编码的蛋白质是protein kinaseRe: 蛋白纯化的脱盐问题
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怎么定义 protein-protein interactionhow to separate denatured proteins from native proteins
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p21 protein (Cdc42/Rac) 和p21 (CDKN1A)是同一个蛋白么?how to store cell lysate for a long time
小白请教, 用Myc tag 做IP好,还是用His tag pull down 好做western的protein lysate浓度太低了大家都怎么浓缩?
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Re: 有人在细菌里表达过cysteine rich protein吗?【包子答谢】标题修改:Histone and Protein Acetyltransferases: Rising Ancient Counterparts to Protein Kinases
相关话题的讨论汇总
话题: kinases话题: denaturing