l*********1
发帖数: 351 | 1 因为要长期使用线性化后的载体，想着冻存后封装成小份使用。不知道双酶切载体后的
粘末端是否稳定？有同事说冻了两年以后还能用，不知道是不是这样？
多谢 |
a****d
发帖数: 1919 | 2 I have fragments stored at -20 for more than one year, they still work fine.
The concentration may decrease overtime though. |
j*****n
发帖数: 217 | 3 my boss is still using his vectors made in 1980s |
q***7
发帖数: 144 | 4 Use this company's gel extraction kit will allow you get washing buffer-free
linearized vectors/DNA fragments, which will increase your cloning
efficiency.
You can store linearized DNA for over 5 years at -20C and still can ligate
to corresponding DNA fragment for over 90%.
http://www.greenbioresearch.com/gel-extraction-pcr-purification |
