v***a
发帖数: 1242 | 1 请问一般普通的组织或细胞中提取的RNA在做RT之前，需要用Dnase I处理吗？如果是
siRNA程序处理过的细胞呢？
谢谢回答！ | D****g
发帖数: 275 | 2 It also depends on what you are going to do with those RNA. If you want to
do Northern blot, No; if you want to do RT-PCR, probably you should; For RT-
PCR, you can design primers to cover intron to amplify the mRNA to avoid the
DNase treatment, or you can use some specially kit to amply cDNA but not
genomic DNA. Good luck. | v***a
发帖数: 1242 | 3 谢谢～
RT-
the
【在 D****g 的大作中提到】
: It also depends on what you are going to do with those RNA. If you want to
: do Northern blot, No; if you want to do RT-PCR, probably you should; For RT-
: PCR, you can design primers to cover intron to amplify the mRNA to avoid the
: DNase treatment, or you can use some specially kit to amply cDNA but not
: genomic DNA. Good luck.
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