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Biology版 - FLAG pulldown needs protease inhibitor ?
相关主题
chip实验求教:大家用0.5%的SDS还是用1%的SDS请推荐一种安全有效的蛋白酶抑制剂
问一个蛋白磷酸化的问题ELISA for ERK and pERK
有人用 in vitro translation 做过co-ip吗?Protein lysate能放在cold room过夜吗?
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endogenous protein ip 时,用antibody crosslinked beads incubate cell lysis 多CoIP- mass spectrum 求助
lysis buffer里加点什么能让tissue在homogenization瞬间蛋白酶就变性了?Flag fusion protein help!
求助,proteasome inhibitorantibody digestion
请教蛋白样品保存是直接冷冻组织好还是冷冻lysis好?请问,癌细胞转移
相关话题的讨论汇总
话题: protease话题: flag话题: inhibitor话题: pulldown话题: needs
进入Biology版参与讨论
1 (共1页)
m******t
发帖数: 109
1
in lysis buffer??
s******s
发帖数: 13035
2
这跟用啥pull down没关系吧。你做cell extract当然都要加,
除非lysis buffer很强

【在 m******t 的大作中提到】
: in lysis buffer??
m******t
发帖数: 109
3
sigma protocol does not mention protease inhibitor at all (i use sigma M2
beads).
and samples need to rotate at RT for 1 hour.
that is why i asked
T*****u
发帖数: 3257
4
totally depends on the stablility of your protein.

【在 m******t 的大作中提到】
: sigma protocol does not mention protease inhibitor at all (i use sigma M2
: beads).
: and samples need to rotate at RT for 1 hour.
: that is why i asked

1 (共1页)
进入Biology版参与讨论
相关主题
请问,癌细胞转移endogenous protein ip 时,用antibody crosslinked beads incubate cell lysis 多
请问有人知道SLC2-Solute carrier family 2的inhibitor吗lysis buffer里加点什么能让tissue在homogenization瞬间蛋白酶就变性了?
请问有p15和p21的inhibitor卖吗?求助,proteasome inhibitor
p21inhibitor会有什么临床功用吗请教蛋白样品保存是直接冷冻组织好还是冷冻lysis好?
chip实验求教:大家用0.5%的SDS还是用1%的SDS请推荐一种安全有效的蛋白酶抑制剂
问一个蛋白磷酸化的问题ELISA for ERK and pERK
有人用 in vitro translation 做过co-ip吗?Protein lysate能放在cold room过夜吗?
RIP 菜鸟求助做RNA binding protein, 选择哪种Protease Inhibitor Cocktail 比较合适?
相关话题的讨论汇总
话题: protease话题: flag话题: inhibitor话题: pulldown话题: needs