l***g
发帖数: 19 | 1 想先做GST-pulldown，然后用pulldown产物电泳银染切胶回收去做MS。问题是，如果我
现在想用细菌中纯化的GST融合蛋白，应该bind多少蛋白到多少的beads上（100ul？）
，然后用HeLa细胞的裂解液来亲和吸附，应该大概用多少HeLa细胞裂解液？我感觉细胞
裂解液少了的话可能结合在GST融合蛋白上的蛋白太少了，在之后洗脱的过程中可能又
损失点，然后在银染的话可能看不出来。如果不用裂解液用动物组织，譬如rat或者
sheep的脑子，大概需要裂解多少克的脑子？多谢！ |
|
s******y
发帖数: 28562 | 2 有一个可能就是你那个全长的蛋白因为一些构象原因不能被pulldown,
反而是被切过的（或者alternatively splice) 的可以被pulldown.
如果你开始用的是native buffer pulldown 的话，不妨换成denaturing pulldown
condition
比方说用 2 %SDS 把细胞给煮了，然后加缓冲液稀释到0.2% SDS再pulldown,
这样一方面可以解决大部分构象原因，另外一方面也会减少背景
当然，如果你比较倒霉的话，这样也有可能导致你什么都pulldown 不下来。这个你得
试一试，就没有人敢给你保证了。 |
|
s***r
发帖数: 358 | 3 Here is how my training looks like:
I train 4 days a week, with 3 days for the main lifts, and a 4th day
dedicated to Abs and some misc assistances. So each of my 531 cycle runs 5
weeks. I train Mon, Wed, Fri and Sat with Sat being the 4th day dedicated
to Abs.
Military Press day
==================
Warm-up:
Agile 8
Band pull-aparts
Shoulder dislocation
Military Press per 531
Back off sets (2x10)
Boring But Big (5x10 at 60% of training max)
Assistance:
Pull-ups: 100 reps (normally 10x10)
Chest su... 阅读全帖 |
|
b*****l
发帖数: 8603 | 4 day008 2-7
mc rest / chinese dance
day009 2-8
mc rest nothing
day010 2-9
chinese dance 2hrs
day011 2-10
swimming 1200m
chinese dance 30mins
day012 2-11
chinese dance 2hrs
day013 2-12
swimming 2000m
torso rotation 30lbs x50 X2
total Ab 30lb x 50
triceps flexation 30lbs x50
day014 2-13
nothing
day015 2-14
swimming 1000m
back row 30lbsx50
back lat 30lbx 50
torso rotation 30lbs x50 x2
day016 2-15
Chinese dance 20mins
day017 2-16
chinese dance 2hrs
swimming 1000m
torso rotation 30lb x50x2
ab crunch 3... 阅读全帖 |
|
b*****l
发帖数: 8603 | 5 day008 2-7
mc rest / chinese dance
day009 2-8
mc rest nothing
day010 2-9
chinese dance 2hrs
day011 2-10
swimming 1200m
chinese dance 30mins
day012 2-11
chinese dance 2hrs
day013 2-12
swimming 2000m
torso rotation 30lbs x50 X2
total Ab 30lb x 50
triceps flexation 30lbs x50
day014 2-13
nothing
day015 2-14
swimming 1000m
back row 30lbsx50
back lat 30lbx 50
torso rotation 30lbs x50 x2
day016 2-15
Chinese dance 20mins
day017 2-16
chinese dance 2hrs
swimming 1000m
torso rotation 30lb x50x2
ab crunch 3... 阅读全帖 |
|
p******l
发帖数: 45 | 6 6.12
Dumbbell Bent-Over Row 10lb 4 x 10
Wide-Grip Pulldown 8lb 3 x 10
Standing Pulldown 8lb 3 x 10
Straight Arm Pulldown 6lb 3 x 10
Hanging Leg Raise 3 x 7
Crunch 3 x 15
spinning 60 min |
|
p******l
发帖数: 45 | 7 健身日记 6.11～6.20
6. 11
Squat 4 x 10;
One-Leg Leg Press 3 x 10;
Leg Extension 3 x 10;
Romanian Deadlift 4 x 10;
Hanging Leg Raise 1 x 10;
Nike training club core 15 min;
Treadmill running: 5k/30 min;
*********************************************************
6.12
Dumbbell Bent-Over Row 10lb 4 x 10;
Wide-Grip Pulldown 8lb 3 x 10;
Standing Pulldown 8lb 3 x 10;
Straight Arm Pulldown 6lb 3 x 10;
Hanging Leg Raise 3 x 7;
Crunch 3 x 15;
spinning 60 min;
****************************************************... 阅读全帖 |
|
s******y
发帖数: 28562 | 8
定了，信号/
I guess in the first case you pulldown your protein and then analyze the
pulldown result by WB? In such case, it would look decent.
In the second case were you trying to pulldown in vivo synthesized protein
with isotope? Indeed, the signal/noise will look awful, because many
endogenous protein also have the radioactive label.
Well, if that's what you want, you probably will need to try the anti-6XHis
antibody. Unfortunately I don't know which antibody works for IP. You should
check this |
|
m*********n
发帖数: 158 | 9 endogenous protein pulldown,以前都是harvest cell lysis，然后antibody incubation overnight, next day add
beads for 1-2hours, and pulldown
听说把beads先和antibody cross link一下，可以提高pulldown efficiency;
所以准备把antibody-bead cross link,加到cell lysis,这样得话，大家一般incubate
在cell lysis里面多久呀？
谢谢 |
|
|
f**y
发帖数: 26 | 11 周一，四，六做力量，二三五专做Cardio，周日Hiking/shopping。
有时周六没时间就只作两次力量。
Cardio日现在是跑40分钟，外加划船5-10，及Stretch 5-10分钟
力量日是跑步20-25分钟，Stretch 5-10分钟，力量大约两个小时。
每次力量训练包括：
Leg Press
Leg Extension
Leg Curl
Bicep Curl
Shoulder Press
Chest Press
Bicep Extension
Lateral Pulldown
Calf Raise
Forearm raise
Cable Pulldown
Sometimes, I also do some AB, Adduction, lower Back and a few other exercise.
锻炼一个月以来我下肢训练的项目重量都增加一半甚至一倍，但上肢训练的项目增加很
有限。 |
|
d*i
发帖数: 9453 | 12 ☆─────────────────────────────────────☆
fjoy (我太有才了) 于 (Tue Jul 17 14:29:23 2007) 提到:
周一，四，六做力量，二三五专做Cardio，周日Hiking/shopping。
有时周六没时间就只作两次力量。
Cardio日现在是跑40分钟，外加划船5-10，及Stretch 5-10分钟
力量日是跑步20-25分钟，Stretch 5-10分钟，力量大约两个小时。
每次力量训练包括：
Leg Press
Leg Extension
Leg Curl
Bicep Curl
Shoulder Press
Chest Press
Bicep Extension
Lateral Pulldown
Calf Raise
Forearm raise
Cable Pulldown
Sometimes, I also do some AB, Adduction, lower Back and a few other exercise.
锻炼一个月以来我下肢训练的项目重量都增加一半甚至一倍，但上肢训练的项目增加很
有 |
|
z********0
发帖数: 9013 | 13 ☆─────────────────────────────────────☆
linkthesky (天使的翅膀) 于 (Mon Mar 21 15:59:00 2011, 美东) 提到:
上周深蹲比平时多练了一组,当时没事,一天后开始腰疼.经过休息现在快一个礼拜了疼
痛感减轻了不少,但是不知道多久才能痊愈到重新投入训练的水平?还有重新开始练要不
要减重,还是接着原来重量继续?
☆─────────────────────────────────────☆
jfjf (慕容若) 于 (Mon Mar 21 16:00:39 2011, 美东) 提到:
悠着点儿。：）
应该过不了多久，小重量先试试呗。：）
千万别转成慢性的伤，比如俺，常年酸疼，没办法。：）
上周深蹲比平时多练了一组,当时没事,一天后开始腰疼.经过休息现在快一个礼拜了疼
痛感减轻了不少,但是不知道多久才能痊愈到重新投入训练的水平?还有重新开始练要不
要减重,还是接着原来重量继续?
☆─────────────────────────────────────☆
gejkl (gejkl) 于 ... 阅读全帖 |
|
R****n
发帖数: 7953 | 14 7.10
力量 二头 背
squat
95*12
incline dumbbell alternating curl
15*12
15*12
30*10
35*8
35*8
40*7
40*6
42.5*5
37.5*6
deadlift
95*12
135*10
135*10
185*8
185*6
225*4
225*4
225*4
cable seated close row
70*10
110*10
110*10
130*8
130*8
150*8
150*8
overhead cable bicep curl
30*15
40*10
55*8
70*8
60*8
high cable chest fly
40*15
55*10
70*10
85*8
70*8
cable lateral pulldown
70*10
110*10
130*8
150*6
cable lateral pulldown(behind neck)
70*10
110*10
130*8
150*6
有氧
run 7.2k |
|
R****n
发帖数: 7953 | 15 7.13
力量 二头 背 下肢
squat
95*12
95*12
deadlift
95*12
95*12
135*10
185*8
225*6
245*5
275*3
225*6
225*6
incline dumbbell alternating
17.5*12
17.5*12
35*10
40*10
40*8
45*8
45*6
45*6
cable seated close row
60*10
110*10
130*8
150*6
cable wide grip seated row
60*10
110*10
130*8
150*6
overhead cable bicep curl
30*15
40*10
55*8
60*6
60*6
high cable chest fly
40*12
55*10
70*10
80*8
cable lateral pulldown
70*8
110*8
130*8
150*4
cable lateral pulldown(behind neck)
70*8
110*8
130*8
150*4
squat
135*10
185*8
235*... 阅读全帖 |
|
R****n
发帖数: 7953 | 16 7.14
力量 肩 三头
dumbbell shoulder press
17.5*15
40*12
45*8
45*6
37.5*8
barbell triceps extension
40*12
70*10
80*10
85*8
85*8
military press(on Smith machine)
25*2+bar * 10
35*2+bar * 10
45*2+bar * 8
50*2+bar * 8
military press(behind neck, on Smith machine)
25*2+bar * 10
35*2+bar * 10
45*2+bar * 8
50*2+bar * 6
triceps cable rope pulldown
40*10
45*10
50*8
55*8
triceps cable bar pulldown
40*10
45*10
50*8
55*8
cable rear shrug
100*10
150*10
150*10
150*10
cable front shrug
100*10
150*8
120*8
cable late... 阅读全帖 |
|
R****n
发帖数: 7953 | 17 7.17
力量 二头 背
deadlift
95*12
135*10
185*10
185*10
235*6
285*1
235*6
incline bicep dumbbell alternating curl
20*12
30*8
32.5*8
35*8
37.5*6
seated bicep barbell curl
50*10
60*10
70*8
70*8
cable seated close row
80*10
120*10
130*8
140*8
150*6
cable wide grip seated row
120*8
140*8
150*8
overhead cable bicep curl
35*12
50*8
60*8
65*6
cable lateral pulldown
100*10
120*8
130*8
140*6
cable lateral pulldown(behind neck)
120*10
130*8
150*6
有氧
run 6.4k |
|
R****n
发帖数: 7953 | 18 7.18
力量 肩 三头 下肢
squat
95*12
95*12
dumbbell shoulder press
20*12
20*12
40*10
42.5*10
42.5*8
45*8
45*6
barbell triceps extension
40*12
70*10
80*10
85*8
85*8
dumbbell lateral raise
10*12
10*12
17.5*10
17.5*10
20*10
22.5*10
triceps cable bar pulldown
40*10
45*10
50*8
55*8
cable rear shrug
120*15
150*15
150*15
150*15
triceps cable rope pulldown
40*10
45*10
50*8
55*8
assisted dip
10*6
military press(on Smith machine)
25*2+bar * 10
35*2+bar * 10
45*2+bar * 8
55*2+bar * 8
military press(behind neck, on ... 阅读全帖 |
|
R****n
发帖数: 7953 | 19 7.18
力量 肩 三头 下肢
squat
95*12
95*12
dumbbell shoulder press
20*12
20*12
40*10
42.5*10
42.5*8
45*8
45*6
barbell triceps extension
40*12
70*10
80*10
85*8
85*8
dumbbell lateral raise
10*12
10*12
17.5*10
17.5*10
20*10
22.5*10
triceps cable bar pulldown
40*10
45*10
50*8
55*8
cable rear shrug
120*15
150*15
150*15
150*15
triceps cable rope pulldown
40*10
45*10
50*8
55*8
assisted dip
10*6
military press(on Smith machine)
25*2+bar * 10
35*2+bar * 10
45*2+bar * 8
55*2+bar * 8
military press(behind neck, on ... 阅读全帖 |
|
h*******t
发帖数: 2679 | 20 我发现一周只能练3次。四次就over train了。我肌肉恢复的很慢。
我是先warm up:
交叉的 15个pullup，bench press bodyweight，abs，四组。
然后才去练pulldown：pulldown machine，和bicep curl或者肩推交叉进行(否则坐在
那里太闲了)；开始从体重-50开始，一点一点的加，每组8个。超过体重就开始用单手
每组5~6个。最高的一组尽可能拉3个。
然后用cool down:
BW-30 pull down；多组
感觉每次都能把上身的某些肌肉弄到极限。
然后去做cardio。 |
|
h*******t
发帖数: 2679 | 21 我发现一周只能练3次。四次就over train了。我肌肉恢复的很慢。
我是先warm up:
交叉的 15个pullup，bench press bodyweight，abs，四组。
然后才去练pulldown：pulldown machine，和bicep curl或者肩推交叉进行(否则坐在
那里太闲了)；开始从体重-50开始，一点一点的加，每组8个。超过体重就开始用单手
每组5~6个。最高的一组尽可能拉3个。
然后用cool down:
BW-30 pull down；多组
感觉每次都能把上身的某些肌肉弄到极限。
然后去做cardio。 |
|
b*****l
发帖数: 8603 | 22 Day84 4-24 nothing
Day85 4-25
Walking 30mins shopping for 1hour
Squats 10mins
Day86 4-26
Walking 30mins
Squats 20mins 10 per group for 4 groups
Day87 4-27
Outdoor walking for 45mins,2miles
Yoga 45mins
Squats 10x2
Day88 4-28
Sick,coughing,headache,rest.
Day89 4-29
Swimming 1000m for 34mins.
Sets~
Torso rotation 50x10x2
Hip adduction 60x10,70x10,80x10
Hip abduction 65x10,70x10,85x10
Lower back 40x10,50x10,60x10
Triceps press 50x5,60x5,70x10
Squat 20lb x10x3
Pulldown 27.5x20
Day90 4-30
Swimming 500... 阅读全帖 |
|
b*****l
发帖数: 8603 | 23 Day84 4-24 nothing
Day85 4-25
Walking 30mins shopping for 1hour
Squats 10mins
Day86 4-26
Walking 30mins
Squats 20mins 10 per group for 4 groups
Day87 4-27
Outdoor walking for 45mins,2miles
Yoga 45mins
Squats 10x2
Day88 4-28
Sick,coughing,headache,rest.
Day89 4-29
Swimming 1000m for 34mins.
Sets~
Torso rotation 50x10x2
Hip adduction 60x10,70x10,80x10
Hip abduction 65x10,70x10,85x10
Lower back 40x10,50x10,60x10
Triceps press 50x5,60x5,70x10
Squat 20lb x10x3
Pulldown 27.5x20
Day90 4-30
Swimming 500... 阅读全帖 |
|
r****y
发帖数: 3412 | 24 我有个trainer给的简单set练上半身的..我给他说是跑步做的补充resistence
training..
seated row
lat pulldown
还有一个三头下压的忘了叫啥.跟pulldown在一套器械里
哑铃curl to press
plank
side plank
leg raise.
基本就是背,二三头加core
如果在家可以整一个medicine ball也很好用 |
|
p***r
发帖数: 4859 | 25 ☆─────────────────────────────────────☆
Meltaways (成真) 于 (Tue Jan 17 11:07:09 2012, 美东) 提到:
关于胸部缩水~~~
我跑了半年，每周三天，每天一小时，五迈上下，然后罩杯就掉了一个size.我很喜欢
跑步，愿意一直这样跑下去到老。但是跑步减胸这一点，让我有点不能承受这项运动。
请问经常跑步的女生们，跑步减胸，是必须的么？有什么补救措施么？深深鞠躬谢过。
☆─────────────────────────────────────☆
ONtheRUN (run it through) 于 (Tue Jan 17 11:33:14 2012, 美东) 提到:
追哥的亲身体验证明蓝的也有同样的问题，大家可以一起探讨。
☆─────────────────────────────────────☆
Trinityy (Trinity) 于 (Tue Jan 17 11:38:47 2012, 美东) 提到:
太在意了就玩点别的吧，看你觉得啥重要了
☆──────────────... 阅读全帖 |
|
t****u
发帖数: 10218 | 26 I wasn't talking about the crystal structure either.:)
I thought in the pulldown exp. you had a GST fusion to pull-down another
version of your protein, is that right? I am concerning about that your
GST-fusions are so geographically close to each other there will be very
little chance to form a GST-protein/protein dimer. Most of them will be
GST-protein/GST-protein dimer, that's why "E coli. 表达纯化的yeast蛋白不能形成
二聚体 （GST pulldown)".
But I'm just speculating, didn't know your exp. detail.
maybe
的修饰 |
|
e****s
发帖数: 1125 | 27 I see.
I cleaned up the samples by ultracentrifuge at 100,000rpm for 30 mins (
without sucrose) following by gel filtration to remove free labeled AAs.
the problem is the pulldown assay signal is very low. I may try both NTA-Ni
pulldown and IP to see which condition give me a better result.
bottom |
|
s******y
发帖数: 28562 | 28 You should NOT use the gel filtration because it will significantly dilute
your sample.
If you have a doubt about this, you should load the eluant directly without
any pulldown to see how much full length protein you recovered.
I don't know why you are bothered by the free amino acid. They will be
washed out in the pulldown process. I suggest you NOT to do the gel
filtration at all.
What you need to do is this:
1.centrifuge to pellet the ribosome. Are you sure you are using 100,000 rpm?
Not 100, |
|
s******y
发帖数: 28562 | 29 I am a little bit confused.
Why don't you use protein A/G to do the pulldown?
Why do you want to use 2nd ab for pulldown? |
|
s******y
发帖数: 28562 | 30 不是具体的分析啦。。。
像我刚才讲的那样，只是一个非常短的小结，或者引出下一个实验来用的句子。
一般是在几个相关的小数据之后才写一次第三点的。不是每一句都必须写。
比方说。
To test whether A possess binding affinity for B, co-immuneprecipitation of exogenously expressed A and B was performed in Hela cells. The results (as shown in Fig 1.A) suggest that A and B can pulldown each other in a reciprocal manner. Pulldown with endogenous A and B was also performed and the result is in well agreement with the former test (Fig 1.B). In order to test whether A binds to B directly, purifie |
|
p*****m
发帖数: 7030 | 31 等明天brain research恢复了我给你贴个例子 呵呵
of exogenously expressed A and B was performed in Hela cells. The results (
as shown in Fig 1.A) suggest that A and B can pulldown each other in a
reciprocal manner. Pulldown wit
binding domain for B, which was not reported before and therefore need to
be elucidated here... |
|
l*****7
发帖数: 153 | 32 请问sunnyday，有没有相关的资料可以学校这种八股文啊？ 谢谢啦 :)
另外我想知道怎么把包子发给你。
of exogenously expressed A and B was performed in Hela cells. The results
(as shown in Fig 1.A) suggest that A and B can pulldown each other in a
reciprocal manner. Pulldown with endogenous A and B was also performed and
the result is in well agreement with the former test (Fig 1.B). In order
to test whether A binds to B directly, purified protein A and B was used
in to
a binding domain for B, which was not reported before and therefore need |
|
s******y
发帖数: 28562 | 33 我不知道这个蛋白是否会泛素化。
但是，蛋白泛素化一般是不能直接看到smear的，因为泛素化的比例一般都不超过该蛋
白的1%。要看smear一般需要同时transfect 6His-Ub, 或者HA-Ubiquitin,
然后要么pulldown his tag and then use your protein antibody to check the
smear, or pulldown your protein and then use HA antibody to check the smear |
|
s******y
发帖数: 28562 | 34 你的老板是对的，看到带升高未必就是走ubiquitin pathway, 不过倒也
不需要用S35 （用了也未必有说服力，因为高分子量的smear未必就是ubiquinylated
band，审稿者要挑毛病还是很容易的）
你可以在MG132 处理（以及对照）的情况下直接pulldown IkBalpha, 然后用anti-
ubiquitin直接来看pulldown product 上的ubiquitin signal。至于抗体，推荐你用
Millipore monoclonal anti-ubiquitin。 这个方法就比较无可挑剔了，也不涉及放射
性。 |
|
R****n
发帖数: 708 | 35 最近有个项目要检测H3 modification和DNA modification的关系，做一些前期的试验
。准备pulldown histone, reversecrolink, 收集DNA做Dot blot.
直到SONICATION这一步都行,Chromatin reverse crosslink后 DNA在agar gel H3 在
page gel上都有信号。 但是pulldown后没有信号。主要基于 Farnham protocol。
他的ChIP buffer就是RIPA,beads也没有block,这个没问题么？有些别的protocol用
salmon DNA block beads,salmon DNA的methylation会影响 DNA methylation dot
blot结果吧？ |
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w*******y
发帖数: 60932 | 36 Alright guys, this is my first deal, but after looking around, the reviews
seem pretty decent and the price seems to be well below what a quick seach
on google gave me, which was $799+. It's B&M only which doesn't help me, but
if you have a FRYS near you this may be a good pick up -- may be limited
supply.
link: Link:
http://www.frys.com/product/6004838?site=sr:SEARCH:MAIN_RSLT_PG
Specs:
Specifications
SUMMARY
Display Type LCD
Screen Size 42"
Screen Size Diagonal 42.0"
Full HD 1080 ... 阅读全帖 |
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w*******y
发帖数: 60932 | 37 Link:
http://www.newegg.com/Product/Product.aspx?Item=N82E16889005149&Tpk=LG 37" 1080p 120Hz LED-LCD HDTV 37LE5300
General
Brand
LG
Model
37LE5300
Cabinet Color
High Gloss Black
Panel
Screen Size
37"
Recommended Resolution
1080p
Maximum Resolution
1920 x 1080
Aspect Ratio
16:9
LED Technology
Edgelit
Viewing Angle
178H) / 178V)
Dynamic Contrast Ratio
3,000,000:1
Response Time
2.4ms
Refresh Rate
120Hz
Video
User Settings
Aspect Ratio Correction: ... 阅读全帖 |
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T*R
发帖数: 36302 | 38 Some Illinois public school teachers earning six-figure salaries
By ROSALIND ROSSI AND ART GOLAB Staff Reporters May 30, 2011 8:48PM
“We’re not just teachers,” says Dirksen Middle School teacher Crystal
Pedroni. “We’re nurses, counselors, we are their parents when their
parents are not there.”
Want to wind up making at least six figures as a public school teacher?
Send your resume to Highland Park or Deerfield High School, both in Township
High School District 113.
The district — which has no te... 阅读全帖 |
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M1
发帖数: 2038 | 40 有哑铃，bench，也有lat pulldown，和low row，以及pull up bar，练shoulder用什
么routine好？ |
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c****g
发帖数: 37081 | 41 擦，问问题也得找对地方。个人觉得哑铃，lat pulldown好点，low row对背，两种
pull up bar，对背，一种对triceps，一种对biceps。triceps非常有用，多多益善；
biceps没啥毛用。 |
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D*******0
发帖数: 4476 | 42 From pulldown menu, find purchase history, then scroll down, then look up
the top bar, you will see cancelled item, cancelled invoice. Then go from
there. |
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m******t
发帖数: 4077 | 43 builder相当的无耻，fireplace也是option， $2500。 值得吗？
很多人说卖的时候没有会比较难卖。
还有attic pulldown ladder需要吗？ $500。自己弄个梯子怎么样啊？ 平时经常需要
上去
吗？
谢谢 |
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s******e
发帖数: 1352 | 44 房子B:
Bedrooms: 4
Bathrooms: 3
Full Baths: 3
Acres: 2.1
Lot Size: 91476
Sq.Ft.: 3,820
Year Built: 1976
Appliance: Dishwasher, Disposal, Hood, Refrigerator, Stove Top, Wall Oven
Fireplace: 2
Pool: In Ground
Architecture Style: Tudor Attic: Pulldown Stairs
Basement: Full Basement Cable Ready: Yes
Cooling System: Central Air Exterior Type: Brick, Stucco
Floor Covering: Wood, Tile, Carpeted Gated Entry: No
Heating Fuel: Oil Heating System: Warm Air
Interior: Foyer, Slider, Wet... 阅读全帖 |
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b****n
发帖数: 3343 | 45 The signup webpage is confusing. See below for the correct way to do it. I
just did it yesterday. It took me a while to figure it out. :)
After you login to your Kohl's online account, move mouse over "ACCOUNT"
under your name on top central of webpage. From the pulldown menu, click "
Yes2You Rewards". Then you can sign up. |
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z***y
发帖数: 7151 | 46 【 以下文字转载自 Fitness 讨论区 】
发信人: Xman0000 (Xman 0), 信区: Fitness
标 题: 【锻炼一年】分享菜鸟健身入门！
发信站: BBS 未名空间站 (Sat Jun 18 05:45:38 2011, 美东)
菜鸟健身入门！
3年前刚来美国的时候，根本也没想过要健身什么的，刚开始学习也很忙，自己做饭做
得太好吃，结果一年多长了40磅肉。暑假去海边玩，拍的照片不能看了，呵呵，当时也
没想那么多，后来发生了一些事，打击很大，心情比较消极，需要转移下注意力，就开
始锻炼了，一练就是一年多，分享点自己的经验，入门级别的。要修炼神功，自宫倒不
必，有些装备还是必须的。
装备：
手套一付
助力带（strap）
Lifting belt (护腰) 有的健身房有借，可以省钱哈
战友：
一个人容易偷懒，有个人或者几个人一起，练起来会好很多。
药品：
刚开始没使用，到了集中减脂的时候采用。
蛋白粉(吃了但是不知道有没有用)
肌酸(还是有点用的，能提供能量，减脂的时候，吃得东西少，肌酸能补充能量，使肌
肉受到比平时大的刺激)
NO （这玩意没用过）
像打游戏一样... 阅读全帖 |
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M**********n
发帖数: 4964 | 47 包子刚给，这customer service居然乱回答我问题，我登陆了，发现你是对的，以前在
pulldown list里，现在没了，现在需要verify DOB 之后，才能有＂Bank Transfer＂
的选项。 |
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l**********5
发帖数: 12 | 48 有氧：走跑交替25 min + 蛙泳 20min (游一会，歇一会)
器械:
Row/ Rear Deltoid 35lb 12*2
Pulldown 40lb 15*2
在gym称还是109lb，在游泳馆称又回到107lb了，希望是gym的称不对，呵呵 |
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h*****b
发帖数: 1 | 49 跟大家比起来，我的运动好像很小儿科啊。
跑步机20分钟，4.0+6.0mph
elliptical 10分钟, level 8
力量几乎没做，就一个，好像叫lat pulldown?
然后就是拉伸，垫子上做了一组pilates的练习butt的动作 ....
~~~~~~~~~~~~
然后吃了一份香茅鸡饭，真是yummy~~~ |
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C*****a
发帖数: 2242 | 50 星期五：
先是一个小时篮球，期间被一个黑人大壮称赞说 This guy knows how to use his
shoulder,另一个黑人高佬看到我抢篮板的积极程度竟然对我高呼今年NBA选秀去芝加哥
的佛大大前锋的名字--Noah。虽然我知道他们有过誉之嫌，不过还是着实为自己的力量
进步得意了一把，哈哈。
之后是腹部训练，135个CRUNCH； 最后是跑步，以放松的8麦速度跑了30分钟
星期六：
引体向上三组：10，8，6个
PULLDOWN三组：115*12，120*10，115*10
T-BAR：45*12，55*12（第二次做，重量还控制不好，感觉还是轻了）
直腿LEG PRESS三组：290*15，310*15，350*15
本来还想接着做硬拉和LEG CURL的，可惜关门时间到了，只得依依不舍的痛别GYM。星
期天一定要恶补大腿后侧的力量练习 |
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