How to design a DNA sequence which doesn't bind any mRNA - Biology版 - 未名存档

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Biology版 - How to design a DNA sequence which doesn't bind any mRNA

相关主题
● Re: questions about DNA and hybridization,thanks a lot~	● 现在一般如何检测ribosome与mRNA的binding效率?
● 请问ChIP之后怎样确定是否拿到有效的DNA片段	● 关于miRNA的一点疑问
● RNA-seq测的也不是绝对表达值吧	● 影响mRNA稳定性的因素有哪些？
● Histone DNA binding	● 请教：transcription factor binding site
● how to create non-redundant DNA sequence dataset	● Re: questions about the latest version of human genome
● EMSA 的问题	● Re: what does "complete cds" mean?
● 请教：如何sequence biotinylated ss DNA oligo?	● 求助:如何找到这个基因的promoter sequence?
● transcription factor binding to one strand of DNA or both strand of DNA?	● How to compare one microRNA sequence with one mRNA sequence?

相关话题的讨论汇总
话题: mrna话题: dna话题: sequence话题: design话题: bind

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l****1 发帖数: 168	1 We need a 10-mer DNA sequence which won't hybridize with any human mRNA sequence. But I design a few and always can find it 100% match in human mRNA database. Can anyone help me on this? Thanks
s******s 发帖数: 13035	2 4^10 = 1M, 基因组3B，你要求太高了。写个程序scan吧 mRNA 【在 l****1 的大作中提到】 : We need a 10-mer DNA sequence which won't hybridize with any human mRNA : sequence. But I design a few and always can find it 100% match in human mRNA : database. Can anyone help me on this? Thanks
s***f 发帖数: 23	3 不能光看sequence, mRNA是形成结构的。

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相关主题
● How to compare one microRNA sequence with one mRNA sequence?	● how to create non-redundant DNA sequence dataset
● Paper help, thanks!	● EMSA 的问题
● 问个计算生物学问题RNAseq / motif finding	● 请教：如何sequence biotinylated ss DNA oligo?
● 准备做RNA sequencing,需要多少的RNA	● transcription factor binding to one strand of DNA or both strand of DNA?
● Re: questions about DNA and hybridization,thanks a lot~	● 现在一般如何检测ribosome与mRNA的binding效率?
● 请问ChIP之后怎样确定是否拿到有效的DNA片段	● 关于miRNA的一点疑问
● RNA-seq测的也不是绝对表达值吧	● 影响mRNA稳定性的因素有哪些？
● Histone DNA binding	● 请教：transcription factor binding site

相关话题的讨论汇总
话题: mrna话题: dna话题: sequence话题: design话题: bind

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