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发帖数: 130 | 1 Methods Mol Biol. 2016;1434:153-67. doi: 10.1007/978-1-4939-3652-6_11.
Protocols for Exosome Isolation and RNA Profiling.
Marqués-García F1,2, Isidoro-García M3,4,5.
Author information
Abstract
Exosomes are small extracellular vesicles of multivesicular bodies derived
from the cell endosome. Molecules have a characteristic composition both in
the membrane and in the carried load. These particles are located in the
culture medium of cells in vitro and in vivo body fluids, being synthesized
by almost all kind of cells. The most characteristic molecule transported
within is the RNA with a large clinical potential. In this chapter, the
methodology for isolation of exosomes and subsequent purification of the RNA
is reviewed. |
