trypsin cut - Biology版 - 未名存档

本页内容为未名空间相应帖子的节选和存档，一周内的贴子最多显示50字，超过一周显示500字访问原贴

Biology版 - trypsin cut

相关主题
● 问一个：蛋白酶为什么不降解它自己？	● Re: 几个问题，大家救命啊。。。
● protein mass spec	● how to create non-redundant DNA sequence dataset
● RNA-seq测的也不是绝对表达值吧	● 请教high-throughput sequencing数据分析
● 如何比较不同物种的同一基因的promoter	● A question about Next Generation Sequencer
● microRNA 能做tissue specific KO吗？	● 求助：老鼠centremere genome position和centremere seqence的数据
● [合集] Trypsinization	● Question about nuclotide frequency measurement in sequenci
● 48／96孔板上养的人胚胎干细胞有没有简易冷冻的方法？	● Short sequence match in a long sequence - help needed
● Re: has maize sequence been sequenced?	● 请教：如何sequence biotinylated ss DNA oligo?

相关话题的讨论汇总
话题: trypsin话题: cut话题: rrrpppgsr话题: site

进入Biology版参与讨论

1

(共1页)

G***G 发帖数: 16778	1 why second R in the following sequences are not trypsin cut site? RRRPPPGSR RRRFHAPIGLVAR
g*****x 发帖数: 3283	2 miscleavage很普遍啊。。。。
G***G 发帖数: 16778	3 I have set the maximal missed cleavage as 0. The second R are not cut site at all. 【在 g*****x 的大作中提到】 : miscleavage很普遍啊。。。。
g*****x 发帖数: 3283	4 你说search fasta的时候？这个一般要设大一点，2。 trypsin的specificity和performance根本没那么好【在 G***G 的大作中提到】 : I have set the maximal missed cleavage as 0. : The second R are not cut site at all.

1

(共1页)

进入Biology版参与讨论

相关主题
● 请教：如何sequence biotinylated ss DNA oligo?	● microRNA 能做tissue specific KO吗？
● HiSeq的下一代sequencer出来了	● [合集] Trypsinization
● rrdw下sequencing中primer和adaptor的区别	● 48／96孔板上养的人胚胎干细胞有没有简易冷冻的方法？
● 请大家推荐一下序列分析软件	● Re: has maize sequence been sequenced?
● 问一个：蛋白酶为什么不降解它自己？	● Re: 几个问题，大家救命啊。。。
● protein mass spec	● how to create non-redundant DNA sequence dataset
● RNA-seq测的也不是绝对表达值吧	● 请教high-throughput sequencing数据分析
● 如何比较不同物种的同一基因的promoter	● A question about Next Generation Sequencer

相关话题的讨论汇总
话题: trypsin话题: cut话题: rrrpppgsr话题: site

未名新帖统计// 7月16日

#	版面	帖数(主题数)
-	全站	4871 (796)
1	Military	3777 (569)
2	Stock	341 (51)
3	Joke	117 (17)
4	History	116 (3)
5	Automobile	100 (9)
6	USANews	55 (9)
7	Midlife	45 (1)
8	Headline	41 (41)
9	Dreamer	33 (13)
10	FleaMarket	32 (20)
11	Living	30 (7)

* 这里只显示发帖超过25的版面，努力灌水吧:-)