x*****n
发帖数: 885 | 1 Hi, I am trying to repeat an experiment from a published paper, but I got
confused about one thing.
The process need a PH 3 solution with 8 M urea. The author adjusted the pH
by 0.1% acetic acid.
However, the PKa of acetic acid is 4.75. Should not the buffer always be
arround 4.75 no matter how much acetic acid I am adding?
In another word, what decides the final pH, the PKa or the concentration of
the acid?
Thanks!
| O*R
发帖数: 609 | 2 both
pH=pKa+log([A]/[HA])
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【在 x*****n 的大作中提到】
: Hi, I am trying to repeat an experiment from a published paper, but I got
: confused about one thing.
: The process need a PH 3 solution with 8 M urea. The author adjusted the pH
: by 0.1% acetic acid.
: However, the PKa of acetic acid is 4.75. Should not the buffer always be
: arround 4.75 no matter how much acetic acid I am adding?
: In another word, what decides the final pH, the PKa or the concentration of
: the acid?
: Thanks!
:
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