n***h
发帖数: 364 | 1 Anyone please explain why the answer is A not D? many thanks. | s********o
发帖数: 3319 | 2 restriction enzyme analysis means the 3 viral DNAs are treated with ONE
restriction enzyme and then the digestion products are loaded onto the gel.
a given restriction enzyme only cuts DNA at a specific sequence called
restriction site. usually a DNA fragment has a limited number of restriction
sites for a given enzyme.
in the above case, the linear HSV-1 viral DNA has 10 sites for the enzyme
they used, which produces 11 DNA fragment after the digestion.
D can be a result from a digestion in which DNA is treated with an
exonuclease that cuts DNA without sequence specificity. | n***h
发帖数: 364 | 3 Thanks for your answer, but still confused. | a******3
发帖数: 1017 | 4 选A吧。
三个sample都是一样的病毒感染，那么他们的酶切位点和相应的pattern应该是一样的
。只有A或者D。
D的pattern是连续的，就是每个位点都切了，不是限制性的（restriction enzyme),只
有A的pattern是针对几个位点的，是限制性的，所以选A. |
|
