C****g
发帖数: 2220 | 1 真是“大道之行简也”。。。
Summary
Differentiated cells can be reprogrammed to an embryonic-like state by
transfer of nuclear contents into oocytes or by fusion with embryonic stem (
ES) cells. Little is known about factors that induce this reprogramming.
Here, we demonstrate induction of pluripotent stem cells from mouse
embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-
Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was
dispensable. These cells, which we designated iPS (induced pluripotent stem)
cells, exhibit the morphology and growth properties of ES cells and express
ES cell marker genes. Subcutaneous transplantation of iPS cells into nude
mice resulted in tumors containing a variety of tissues from all three germ
layers. Following injection into blastocysts, iPS cells contributed to mouse
embryonic development. These data demonstrate that pluripotent stem cells
can be directly generated from fibroblast cultures by the addition of only a
few defined factors.
http://www.sciencedirect.com/science/article/pii/S0092867406009 | M*****n
发帖数: 16729 | 2 日本人写的英文很多都是如此。
(
c-
stem)
express
【在 C****g 的大作中提到】
: 真是“大道之行简也”。。。
: Summary
: Differentiated cells can be reprogrammed to an embryonic-like state by
: transfer of nuclear contents into oocytes or by fusion with embryonic stem (
: ES) cells. Little is known about factors that induce this reprogramming.
: Here, we demonstrate induction of pluripotent stem cells from mouse
: embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-
: Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was
: dispensable. These cells, which we designated iPS (induced pluripotent stem)
: cells, exhibit the morphology and growth properties of ES cells and express
| m**i
发帖数: 9848 | 3 yeah, you just need to get your job done, no nonsenseness. | m*****5
发帖数: 23482 | 4 这不是写得挺好的吗，清楚明白，没有绕来绕去的废话 |
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