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发帖数: 5697 | 2 Chloroquine, a widely-used anti-malarial and autoimmune disease drug, has
recently been reported as a potential broad-spectrum antiviral drug.8,9
Chloroquine is known to block virus infection by increasing endosomal pH
required for virus/cell fusion, as well as interfering with the
glycosylation of cellular receptors of SARS-CoV.10 Our time-of-addition
assay demonstrated that chloroquine functioned at both entry, and at post-
entry stages of the 2019-nCoV infection in Vero E6 cells (Fig. 1c, d).
Besides its antiviral activity, chloroquine has an immune-modulating
activity, which may synergistically enhance its antiviral effect in vivo.
Chloroquine is widely distributed in the whole body, including lung, after
oral administration. The EC90 value of chloroquine against the 2019-nCoV in
Vero E6 cells was 6.90 μM, which can be clinically achievable as
demonstrated in the plasma of rheumatoid arthritis patients who received 500
mg administration.11 Chloroquine is a cheap and a safe drug that has
been used for more than 70 years and, therefore, it is potentially
clinically applicable against the 2019-nCoV. |
