l****b
发帖数: 400 | | I****s
发帖数: 1167 | 2 我记得咱俩争论过新冠患者中的igG抗体是否是中合性抗体，这下应该有定论了吧？
One antibody (named S309) potently neutralizes SARS-CoV-2 and SARS-CoV
pseudoviruses as well as authentic SARS-CoV-2, by engaging the receptor-
binding domain of the S glycoprotein. Using cryo-electron microscopy and
binding assays, we show that S309 recognizes an epitope containing a glycan
that is conserved within the Sarbecovirus subgenus, without competing with
receptor attachment. Antibody cocktails that include S309 in combination
with other antibodies that we identified further enhanced SARS-CoV-2
neutralization, and may limit the emergence of neutralization-escape mutants
. These results pave the way for using S309 and antibody cocktails
containing S309 for prophylaxis in individuals at a high risk of exposure or
as a post-exposure therapy to limit or treat severe disease.
.....
To further investigate the mechanism of S309-mediated neutralization, we
compared side-by-side infection of SARS-CoV-2–MLV in the presence of either
S309 Fab or S309 IgG. Both experiments yielded comparable IC50 values (3.8
and 3.5 nM, respectively), indicating similar potencies for IgG and Fab (Fig
. 3c). However, S309 IgG-mediated neutralization reached 100%, whereas
neutralization plateaued at about 80% in the presence of S309 Fab (Fig. 3c).
This result indicates that one or more IgG-specific bivalent mechanisms—
such as S-glycoprotein trimer cross-linking, steric hindrance or aggregation
of virions44—may contribute to the ability of S309 to fully neutralize
pseudovirions. | l****b
发帖数: 400 | 3 和你说的有毛线关系？我清清楚楚地告诉过你，抗体未必中和。只有分离出中和抗体才
能有预防和治疗的意义。
glycan
mutants
【在 I****s 的大作中提到】
: 我记得咱俩争论过新冠患者中的igG抗体是否是中合性抗体，这下应该有定论了吧？
: One antibody (named S309) potently neutralizes SARS-CoV-2 and SARS-CoV
: pseudoviruses as well as authentic SARS-CoV-2, by engaging the receptor-
: binding domain of the S glycoprotein. Using cryo-electron microscopy and
: binding assays, we show that S309 recognizes an epitope containing a glycan
: that is conserved within the Sarbecovirus subgenus, without competing with
: receptor attachment. Antibody cocktails that include S309 in combination
: with other antibodies that we identified further enhanced SARS-CoV-2
: neutralization, and may limit the emergence of neutralization-escape mutants
: . These results pave the way for using S309 and antibody cocktails
| I****s
发帖数: 1167 | 4 我们上次争论的就是igg抗体是不是中合性抗体。因为abott的抗体测试是测试换igg抗
体的。 | l****b
发帖数: 400 | 5 那么，你认为abbott测的是中和抗体么？
【在 I****s 的大作中提到】
: 我们上次争论的就是igg抗体是不是中合性抗体。因为abott的抗体测试是测试换igg抗
: 体的。
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