f*1
发帖数: 837 | 1 If one isn't worried about chemical applications, the following 2 articles
have some suggestions, although it may end up with expensive multi-year
chemical spraying projects.
http://archive.lib.msu.edu/tic/golfd/article/2008mar80.pdf
The best treatment was fenoxaprop-P plus triclopyr plus mesotrione. This
combination reduced bermudagrass cover from 71 percent when the study
started to 2 percent when evaluated mid-summer in the next season. The
majority of the plots treated with this combination ... 阅读全帖 |
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b*s
发帖数: 82482 | 5 In the 1970’s and 80’s studies started to show that dishes with lead in
the glaze could leach lead into foods. In response, the Consumer Products
Safety Commission and the Food and Drug Administration set standards for the
amount of lead that could be used in products associated with food
preparation. Today, most American manufactures have stopped using lead in
products like ceramic cooking ware and dishes, but there are no regulated
standards for lead in other glazed products like sinks and tub... 阅读全帖 |
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h**********0
发帖数: 45 | 10 【 以下文字转载自 QueerNews 讨论区 】
发信人: unfair (unfair), 信区: QueerNews
标 题: 为了后代不“被同性恋” 让自由党下台 (转载)
发信站: BBS 未名空间站 (Sat Feb 11 11:45:15 2012, 美东)
发信人: unfair (unfair), 信区: Canada
标 题: 为了后代不“被同性恋” 让自由党下台
发信站: BBS 未名空间站 (Sat Feb 11 11:45:01 2012, 美东)
（加中网 - 加拿大中文门户网 WWW.CACNW.COM 讯）
我曾经是自由党（联邦和安省）的支持者，但是，由于“性教育”问题，我不得不反对
自由党，因为这关系到我（以及公众）的家庭和子孙后代的前途。
日前听朋友说，安省自由党正着手在安省教育系统中贯彻“同性恋教育”，他向我推荐
“银禧社”网站（jccsa.ca）题为“多倫多教育局“挑戰害怕同性戀反異性戀主義(
Challenging Homophobia and anti-Heterosexism)政策”的新霸權主義”的文章，阅
后让我十分惊异和困惑。今日又... 阅读全帖 |
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m******e
发帖数: 1293 | 15 14 pounds of Arm & Hammer Kitty Litter $4.99 with free shipping, Use Code:
GREATFREE
Choose from Unscented Clumping Litter, Fresh Scent Clumping Litter and Multi
-Cat Fresh Scent Clumping Litter.
You can buy up to 5 of these- 70 pounds for $24.90 with the free shipping
coupon.
http://www.greatcleaners.com/Products/Arm%20and%20Hammer?CID=cj |
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s*******y
发帖数: 46535 | 19 把music版的帖子借过来
发信人: MagicAnt (魔法蚂蚁), 信区: Music
标 题: 【技术】贴在线视频和音频方法
发信站: BBS 未名空间站 (Tue Dec 18 11:03:34 2007), 转信
【用 box.com 贴 mp3 文件】
在 box.com 贴 mp3 时音质没有损失，推荐大家使用，当然也欢迎推荐其他的方法：）
（转自Vieux）
mp3 文件可以上传到 box.com，
此网站存放的mp3文件音质受损的程度小。
上传成功后，留意一下"All Files and Folders"下的歌曲文件。
歌曲文件的右侧，有一个倒三角，点击此三角，
Share -> Embedded File in Your Site
复制代码，
提取 https 至 swf 的部分，删去 https 的 s，加前缀 v 即可。
e.g.
如果链接是 https://www.box.net/embed/xxxxxx.swf
那么发贴时用的代码就是 vhttp://www.box.net/embed/xxxxxx.swf
注意发帖时 vhttp 的 v 要放在第一列，前... 阅读全帖 |
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D*****r
发帖数: 6791 | 21 Acts 3:11-26
Peter Speaks to the Onlookers
11While the beggar held on to Peter and John, all the people were
astonished and came running to them in the place called Solomon's Colonnade.
12When Peter saw this, he said to them: "Men of Israel, why does this
surprise you? Why do you stare at us as if by our own power or godliness we
had made this man walk? 13The God of Abraham, Isaac and Jacob, the God of
our fathers, has glorified his servant Jesus. You handed him over to be
killed, and you disown... 阅读全帖 |
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D*****r
发帖数: 6791 | 22 Acts 22:1-21 保罗的自辩
1“Brothers and fathers, listen now to my defense.”
2When they heard him speak to them in Aramaic, they became very quiet.
Then Paul said: 3“I am a Jew, born in Tarsus of Cilicia, but brought up in
this city. Under Gamaliel I was thoroughly trained in the law of our fathers
and was just as zealous for God as any of you are today. 4I persecuted the
followers of this Way to their death, arresting both men and women and
throwing them into prison, 5as also the high priest and all t... 阅读全帖 |
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l**********t
发帖数: 5754 | 23
生活主题，如果有中文作者写的，那就更好了，谢谢
FYI -- an old post from the BibleStudy club.
-----------------------------------------
发信人: wmatthew (马太), 信区: BibleStudy
标 题: 《属灵古典名著评介》所介绍的101本好书 的电子书地址
发信站: BBS 未名空间站 (Fri Dec 24 13:29:25 2010, 美东)
陈希曾弟兄《属灵古典名著评介》中所介绍的101本好书 的大部分电子书下载地址(主
要是英文)
1. Henry Alford 《Greek Testament》《Greek Testament for English Readers》
（已绝版）：帮助我们对圣经原文有进一步的认识，作为一整套的新约commentary值得
推荐，对原
文的解释很好的工具书.
第一冊：四福音
http://books.google.com/books?
id=YOY2AAAAMAAJ&printsec=frontcover&dq=.+Henry+Alford+... 阅读全帖 |
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M******l
发帖数: 443 | 30 netstat 只是告诉你当前本机建立的tcp/ip connections, 包括本机端口, remote
地址和端口.
最大的用处是当别人icq上disable ip display时, 可以msg him/her然后查出his/her
ip and port, and bomb him/her.
http://neworder.box.sk/box.
php3?gfx=neworder&prj=neworder&key=icq&txt=ICQ%20exploits%20and%20utils
some tools about icq nuking. |
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T**********r
发帖数: 287 | 31
inducible
多谢提醒，不过你提到这个是openbiosystem的另外一个系统TRIPZ，的确是用
tetracyline
induce的。
Open biosystem有两种shRNAmir系统实现knock down:
a.Stable knockdown: GIPZ；shRNAmir is constitutively expressed as a
polycistronicvector that codes for tGFP, puromycin resistance
marker (Puror) and the shRNAmir.
b.Regulated knockdown: pTRIPZ,也是你提到的。
见链接
http://www.openbiosystems.com/collateral/rnai/pi/Lentiviral%20shRNAmir%2
0for%20Stable%20and%20Regulatable%20RNAi.pdf
再次真诚感谢，有了质疑，才能避免将来实验走弯路。 |
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v**********m
发帖数: 5516 | 32 The GST-tag will artificially increase the solubility of unfolded proteins
fused with a GST-tag, however after the GST-tag is chopped off by a
protease (mostly likely specific ones) the unfolded target proteins will
precipitate out in solution.
GST-tag has nothing to do with the toxicity of a certain protein.
The truth is that most of mammalian proteins or mutant proteins are not 100%
soluble when they are over- expressed in bacteria. What you may do is to
increase the portion of soluble protein... 阅读全帖 |
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f*****e
发帖数: 223 | 33 哈医大专家周春水发现乳腺癌新型抑癌基因
http://www.hljnews.cn 2011-03-23 来源：黑龙江新闻网-黑龙江日报
黑龙江新闻网讯 在本月世界顶尖级生物医学杂志《CELL》上，哈佛医学院与哈医
大专家关于PTPN12蛋白为新型抑癌基因的最新研究成果引起国际肿瘤界的普遍关注。
PTPN12是哈佛医学院发现的一个阳性蛋白分子。为了证明该蛋白分子与癌细胞的作
用关系，专家们用小核苷干扰技术抑制PTPN12表达，结果发现PTPN12表达水平降低会引
起正常细胞恶性生长，反之其过量表达可对恶性细胞的生长有一定的抑制作用。哈医大
医学遗传学教授周春水通过实验发现在PTPN12表达受到抑制时，乳腺癌的促癌基因EGFR
受体家族的络氨酸磷酸化有显著增强，而EGFR受体家族正是经过一系列络氨酸磷酸化而
被激活，产生促细胞癌变效应，从而周春水教授成功地确定了PTPN12是一种新型抑癌基
因。
http://www.ncbi.nlm.nih.gov/pubmed?term=zhou%20chunshui%20and%2
Results: 3
1.Activation o... 阅读全帖 |
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l**********1
发帖数: 5204 | 35 454 GS FLX+
● Up to 1000bp
● 99.997% accuracy
● 1M reads, 700bp
0.7 Gbase/day
● Homopolymer issue
● Cost ~$14K for run
● $500K instrument
$20K/Gbase
---
or soon later you can try:
Ion Torrent PGM
● Long Reads and Fast
● No optics, CMOS chip sensor
● Uses standard nucleotides
● Up to 300bp reads (going to 400bp)
● >99.5% raw
● >99.999% consensus
● 318 (11M wells) >1Gbase in 3 hours
● 8 Gbase/day possible
● Micron sized well, 22mmx22mm chip
moving to 1 Billion wells per chip
● $700 per run (1 Gbas... 阅读全帖 |
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l**********1
发帖数: 5204 | 36 BOOK:
//www.amazon.co.uk/gp/search?index=books&linkCode=qs&keywords=354069918X
Free
Google Book Link
//books.google.co.uk/books?id=EnmrDv1cMVkC&pg=PA120&lpg=PA120&dq=Molecular+
architecture+of+smell+and+taste+in+Drosophila+book&source=bl&ots=pOeTK-_cPS&
sig=oZ2uOx-U6LL0WZeBixqQiDM-HIo&hl=en&sa=X&ei=Z8lDT-f5Mc774QTU4qSrCA&ved=
0CEcQ6AEwBQ#v=onepage&q=Molecular%20architecture%20of%20smell%20and%20taste%
20in%20Drosophila%20book&f=false
免费的最近的书 全文电子版的
若你那单位ip 无法下载全文
您就别做梦搞到那个了 mega uplpoad 年初被封了以后... 阅读全帖 |
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l**********1
发帖数: 5204 | 37 15 min 4℃
Both
TRIzol@
//tools.invitrogen.com/Content/SFS/ProductNotes/F_071215_Trizol%20and%20Trizol%20LS.pdf
or
ISOGENE@
//www.nippongene.com/pdf/manual_english/ISOGEN_LS_2_091001.pdf |
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l**********1
发帖数: 5204 | 38 Please go to
//www.biomedexperts.com/Profile.bme/1623883/Xiaowei_Zhuang
or
//lib.bioinfo.pl/find?field=Papers&query=zhuang+xiaowei
or
//www.ncbi.nlm.nih.gov/pubmed?term=(ZHUANG%20X%5BAuthor%5D)%20AND%20Harvard%
5BAffiliation%5D
or for those every weekly/biweekly CNS Cover Article/Report
please go to
//twitter.com/#!/sciencenewsorg
//twitter.com/#!/naturenews
//twitter.com/#!/cellcellpress |
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l**********1
发帖数: 5204 | 39 Harvard
only one ZHUANG X almost at last author/corresponding author or last 2nd author position,
so plus Affiliation then should be ok. Ok?
//www.ncbi.nlm.nih.gov/pubmed?term=(ZHUANG%20X%5BAuthor%5D)%20AND%20Harvard%
5BAffiliation%5D |
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l**********1
发帖数: 5204 | 41 Genomic DNA plus protein (some RNase + enzyme)
contaminated within your Total RNA extraction sample
from gel picture: the most near
414004.jpg
(160922 字节)
that shining line is Genomic DNA absolutely.
for good RNA extraction protocol
please go to
//tools.invitrogen.com/content/sfs/productnotes/F_Trizol%20and%20Trizol%20LS-041018-RD-TL-
HL0506021.pdf |
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l**********1
发帖数: 5204 | 42 sunnyday
实事求是一点，行不？
before you can got Nobel or at least Lecture professorship likes him
Alfred I. Tauber
//en.wikipedia.org/wiki/Alfred_I._Tauber
//people.bu.edu/ait/
just be 实事求是一点，行不？
Do not tell me that Matrix Can do that similar things of AI Tauber did them.
Ps:
if you have interests to
Artificial immune systems
please just go to
one paper:
Julie Greensmith J et al.
Artificial immune systems
//core.kmi.open.ac.uk/display/381619
or
Alfred I. Tauber
his a chapter of one book:
"Immuonology an... 阅读全帖 |
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l**********1
发帖数: 5204 | 44 不客气
plus
Medical diagnostics
Dynamical diseases
Heart rate variability
Cardio-vascular model
at
NONLINEAR DYNAMICS AND CHAOS (2007)
by Patrick E McSharry
University of Oxford
web link:
HTTP : //people.maths.ox.ac.uk/mcsharry/lectures/ndc/lecture11notes.pdf
4. Fractals and Attractors
5. Bifurcations
6. Quantifying Chaos
7. Nonlinear Time Series Analysis
8. Nonlinear Modelling and Forecasting
HTTP : //people.maths.ox.ac.uk/mcsharry/lectures/ndc/lecture1notes.pdf
and one bible book:
Non... 阅读全帖 |
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