B*Q
发帖数: 25729 | 1 有人说牛奶蛋白(casein protein)有致癌作用。不知是不是真的。不管是真是假，我反
正不喜欢牛奶，干脆就不吃奶制品了。市场上的肉食，大多安全品质有问题（抗生素，
荷尔蒙），于是，肉也省了。 |
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R*****s
发帖数: 41236 | 2 很多健身，跑步完的人的最佳食物就是chocolate milk, 完美carb, protein配比...
而且同样多的卡路里，显然脱脂奶非常涨肚子...
牛奶里面的protein主要是casein，吸收比较慢，让人体慢慢一直吸收...
drinking |
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j******n
发帖数: 871 | 3 actually, I agree a lot of points in Chi's article. And I appreciate his
effort.
And I do think he can do better with modification of his method, by just
balancing and spread out his protein intake, same total cal intakes as he
does right now. I guess the result would be much better.
for example, 30 g protein in the afternoon can be split up into 15g
afternoon and 15g before bed, but change from whey to casein or hemp,
accompany with fibre rich cookie or plain fibre in both.
just my 2 cents. |
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a***a
发帖数: 40617 | 4 oyeah
whey是fast的
casein是slow的
先整whey吧 |
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b***i
发帖数: 10018 | 5 bodybuilder也有vegetarian的，肯定比吃casein的健康吧？ |
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p*********l
发帖数: 26270 | 6 1和3需要吃casein这样的缓释的蛋白粉，吃肉也可以
2需要吃whey这样的快速的蛋白粉，混在smoothie之类的碳水里面吃，或者搭配别的高
GI碳水一起吃，会增加利用率
牛奶里面蛋白质还没碳水多呢，一勺粉顶三四杯牛奶的蛋白质，不要比啦，西西 |
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b***u
发帖数: 12010 | 7 whey粉的速度是食物没法比的，练完必须吃。其他时间随意。我觉得casein挺助睡眠的
，当安眠药吃。 |
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h****8
发帖数: 599 | 8 训练前后都要吃 睡前吃casein protein |
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h****8
发帖数: 599 | 9 训练前后都要吃 睡前吃casein protein |
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c*********y
发帖数: 1461 | 10 lz好牛！！皂很美！！不过说得羊奶跟神一样，牛奶是渣，就好像不太合适吧，虽然我
知道mm估计也就是哪里摘抄来的。。。只挑了我觉得最扯淡的几条说一下
2、 羊奶含有丰富的乳清蛋白，牛奶没有；
牛奶有
Cow's milk is 20% whey protein and 80% casein protein
来自 wikipedia
6、 羊奶的脂肪为不饱和脂肪，牛奶的脂肪为饱和脂肪；
多少比例的问题，不是说一个有一个没有
in 100 grams milk Cow Goat Sheep Water Buffalo
grams Protein 3.2 3.1 5.4 4.5
grams Fat (Saturated) 2.4 2.3 3.8 4.2
grams Fat (Mono-unsaturated) 1.1 0.8 1.5 1.7
grams Fat (Polyunsaturated) 0.1 0.1 0.3 0.2
虽然是非学术网站摘的数据，但是既然有... 阅读全帖 |
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s******n
发帖数: 459 | 11 先声明一下啊，偶不吃肉和海鲜，蛋奶素。但是其实喝牛奶的残忍程度也不下于吃veal
了:
http://en.wikipedia.org/wiki/Veal
Newborn calves are given a varied amount of time with their mothers, which
can be anything from a few hours to a few days.[8][9] Free-raised calves are
raised alongside their mothers, and always have access to their mother’s
milk.
The modern veal industry has strong connections with the dairy industry.[8][
9] To produce milk, cows must be lactating, and to be lactating, they must
get pregnant and give birth. Approximat... 阅读全帖 |
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K****N
发帖数: 10783 | 12 casein蛋白据说在睡觉的时候，也给长肌肉。有一次看到打折，不过没有没有买。
of
In |
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h*******o
发帖数: 4884 | 13 Re-use
Use Casein or Pierce Superblock to dilute antibody, so it can be re-used and
stored at 4oC for longer time without precipitation
Also, use signal enhancer |
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s******y
发帖数: 28562 | 14 你的那种绿色的膜背景很高啊，不知道你用的是不是Nitrocellulose?
如果你不小心用错了PVDF，会导致背景非常高！
另外，最好不要用牛奶。我的经验是，要么就用牛奶里面提纯的那个casein，
那么就乖乖的用Licor 产的blocking buffer。直接用牛奶的话也很容易导致
背景很高。
在我的经验里，Licor Odessey 的灵敏度比Femto kit稍微低一点点，但是
绝对比普通的ECL强的多 |
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s******r
发帖数: 2876 | 15 是PVDF，millipore non-fluoscence的，用intensity 7.5，背景已经很高了，
anyway，想省钱大概是不行了。
你的那种绿色的膜背景很高啊，不知道你用的是不是Nitrocellulose?
如果你不小心用错了PVDF，会导致背景非常高！
另外，最好不要用牛奶。我的经验是，要么就用牛奶里面提纯的那个casein，
那么就乖乖的用Licor 产的blocking buffer。直接用牛奶的话也很容易导致
背景很高。
在我的经验里，Licor Odessey 的灵敏度比Femto kit稍微低一点点，但是
绝对比普通的ECL强的多 |
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s******r
发帖数: 2876 | 16 每个都想想配成0.5%，但是加热到一百度都还没有全溶解。
大家一般怎么弄得？ |
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s******y
发帖数: 28562 | 18 Rotate overnight.
No need to heat in our lab
Sometimes (depending on the applications) we add 0.5% tween-20 in it and
it will help to dissolve the protein significantly |
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s******r
发帖数: 2876 | 19 我看着不溶，autoclave了半小时，还是没动静，又转了一晚上，看来是溶不了了。
stir + heat over night |
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s******r
发帖数: 2876 | 20 能不能请教一下浓度和产品catalogue number，
会不会浓度低一些能溶。
Rotate overnight.
No need to heat in our lab
Sometimes (depending on the applications) we add 0.5% tween-20 in it and
it will help to dissolve the protein significantly |
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s******y
发帖数: 28562 | 21 你autoclave gelatin 了？
哎呀那可完了。
那个鬼东西如果你在用室温化开之前去煮它，就会变性凝在一起，这下你可就
再也溶不了了。
你应该用室温，先转一个晚上，然后再加热到５０度左右大概两三个小时。
在彻底溶完之后，过滤，然后再autoclave |
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s********n
发帖数: 248 | 22 个人感觉灵敏度比film稍差。他们blocking buffer里用的是casein， 具体配方不明。
。。
扫膜的时候等的时间比较长 |
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p*******n
发帖数: 63 | 23 这篇文献没有电子版，但对我来说很重要，求求好心人帮帮忙！
Author(s): VANDIJK HJM
Source: NETHERLANDS MILK AND DAIRY JOURNAL Volume: 46 Issue: 2 Pages: 101-
113 Published: 1992
Title：THE PROPERTIES OF CASEIN MICELLES .6. BEHAVIOR ABOVE PH-9, AND
IMPLICATIONS FOR THE MICELLE MODEL
thanks a lot!!
z**********[email protected] |
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k***g
发帖数: 4904 | 24 Celltracker Orange染料说明上说要avoid amine- and thiol-containing buffers，
我就不知道配working solution时候用的media到底选啥好，培养E.coli的LB Broth算
amine- and thiol-containing buffer吗，配方Tryptone：Yeast Extract：NaCl=2:1:
2 （Luria），或者Enzymatic Digest of Casein：Yeast Extract：Sodium Chloride=
2:1:1（LENNOX） |
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i*********0
发帖数: 915 | 26 Cell Research上的一个AOP稿子。
Retraction: Defective heart development in hypomorphic LSD1 mice
Thomas B Nicholson, Hui Su, Sarah Hevi, Jing Wang, Jeff Bajko, Mei Li,
Reginald Valdez, Joseph Loureiro, Xiaodong Cheng, En Li, Bernd Kinzel, Mark
Labow and Taiping Chen
The authors unanimously wish to retract this paper because the calcium/
calmodulin-dependent protein kinase 2 (CamKII) was erroneously equated with
CK2 (Casein Kinase 2) throughout the paper. While all the results were valid
and supported th... 阅读全帖 |
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s****a
发帖数: 436 | 27 Thank you. Your suggestion is very helpful.
I agree the strong affinity between Fe2O3 and ZnSe-COOH.
I think I have two options:
1. try B-casein or BSA to block the surface of MNP-COOH.
2. Increase the surface DNA density to prevent the non-specific adsorption.
your
Did
to aggregate. |
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f****n
发帖数: 95 | 31 我在为含氟的丙烯酸酯/丙烯酰胺类高分子寻找good solvent,发现trifluoroacetic
acid (TFA)的溶解能力特别强，很好用。可是，高分子（白色粉末）溶解于新鲜的TFA
之后溶液由无色透明慢慢变为淡黄色透明。
是不是因为TFA是强酸有所谓的发烟现象？浓盐酸就是淡黄色，因为含有少量杂质Cl2
。另外， 含卤素或含硝基的化合物容易变颜色,是少量变质导致的吗？
谢谢！
p.s.
查到有这么一句：“olutions of proteins in trifluoroacetic acid prepared in
vacuo
are clear and colourless; on exposure to air, a purple colour develops.”不
解中···
Nature 174, 509 (11 September 1954); doi:10.1038/174509a0
Anhydrous Trifluoroacetic Acid as a Solvent for Proteins
JOSEPH J. KATZ
Chemistry Di... 阅读全帖 |
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w******e
发帖数: 953 | 32 Journal of Dental Research
Volume 76, Issue 9, 1997, Pages 1587-1595
Remineralization of enamel subsurface lesions by casein phosphopeptide-
stabilized calcium phosphate solutions (Article)
Reynolds, E.C.
Biochemistry and Molecular Biology Unit, School of Dental Science,
University of Melbourne, 711 Elizabeth St., Melbourne, Vic. 3000, Australia |
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w*******y
发帖数: 60932 | 34 BSN Syntha-6 Ultra-Premium Sustained Release Protein Powder, Vanilla Ice
Cream, 2.91-Pound Jar
$18.61
Free shipping with Subscribe and Save. Just cancel once you get your order.
Link:
http://www.amazon.com/dp/B000GP5HJI/ref=pe_88830_16457170_pe_epc_d1
Great tasting protein, blend of casein/whey, perfect for me post workout or
just between meals.
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w*******y
发帖数: 60932 | 35 Happy Labour Day
I know this deal is fairly perpetual, but I figured I'd (re)post it as all
the colours are in stock (which is the rare element of this deal). Use code
ULO176 for $5 off for new customers (note - to purchase more than one
bottle, or if you've ordered from iherb before, you'll need to make multiple
accounts).
These are amazing for mixing any kind of powdered beverage, most notable
protein, which is often clumpy. Even casein mixes well with these! I've
also used these for co |
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w*******y
发帖数: 60932 | 38 www.dailydeals.net:
http://www.dailydeals.net
Cinnamon Roll, Chocolate Peanut Butter, Vanilla Yogurt
This PowerBar pack contains twelve 2.75oz Protein Plus bars. Each bar
provides 23g of Trisource protein, a fortifying blend of soy, wheat, and
casein proteins. In addition, each bar contains 100% of the recommended
daily values for 10 essential vitamins and minerals, plus loads of important
vitamins and carbohydrates to nourish muscles and encourage faster recovery
. Protein Plus bars are ideal f... 阅读全帖 |
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w*******y
发帖数: 60932 | 41 GASPARI NUTRITION - IntraPro Delicious Vanilla 5 lbs $26.99+ $5.95 s/h
Link:
http://www.allstarhealth.com/f/gaspari_nutrition-intrapro.htm
Supplement Facts
Serving Size: 1.00 scoop(36g)
Servings per Container: 63.00
Ingredient Amount % Daily Value**
Calories 155
Calories from Fat 31.5 5
Total Fat 3.5g 5
Saturated Fat 0.5g 2
Trans Fat 0
Cholesterol 45mg 15
Sodium 70mg 3
Potassium 150mg 4
Total Carbohydrates 5g 2
Dieta... 阅读全帖 |
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w*******y
发帖数: 60932 | 43 I haven't used this stuff yet myself but just ordered 5 based upon
recommendations and the sale price on the 35 servings tub. Also, the 9.2
rating on BB.com seems pretty reassuring.
Keep in mind the website gives away a selection of free gifts for orders
over $75, $100, and $150 price points. You will see this clearly as you
order, better gifts the more you spend obviously.
Shipping seems to be between 7-10$ for me depending on what I had in my cart
, but the shipping went up very little with ... 阅读全帖 |
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w*******y
发帖数: 60932 | 44 My first deal I've posted here, please take it easy on me!
Shopping for protein this evening I stumbled across the following deal:
Dymatize Elite Whey Protein Isolate- 10 lbs for $68.34 -- free ship w/ S & S
The only flavor at this price is the "berry blast", which seems to have
mixed reviews (7.3/10 at BB.com). I'm fine with it since I usually just
slam it down post WO anyway.
This is a different product than the elite gourmet that was posted recently
at $72 for 10 lbs- this specific protein i... 阅读全帖 |
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