S*******l
发帖数: 4637 | 1 这样也行？那我也可以邀功了，第一个提出抗艾滋病protease inhibitor防治新冠。
http://www.mitbbs.com/article_t/Military/55627213.html
3C protease inhibitor是抑制鼻病毒蛋白酶的。SARS的蛋白酶和3C protease 很像，
3C protease inhibior对SARS 病毒也有效。
上次没赶上试用，这次应该给重症病人使用。
nelfinavir是上市的抗艾滋病药物，对SARS复制有抑制作用，可以直接上了。
加到用药方案里。 |
|
z*******4
发帖数: 285 | 2 算了，你脑子大概也被活摘过
懒得跟你解释，贴个清单吧：
累计研究数量 注册号 注册日期 研究者单位 研究者单位（英文） 注
册题目 注册题目（英文) 预计纳入参试者日期
1 ChiCTR2000029308 1/23/2020 武汉市金银潭医院（武汉市传染病医院）
Wuhan Jinyintan Hospital (Wuhan Infectious Diseases Hospital) 一项评价
洛匹那韦/利托那韦和干扰素-α2b联合治疗武汉新型冠状病毒肺炎(COVID-19)住院患者
的疗效和安全性随机、开放、空白对照的研究 A randomized, open-label, blank-
controlled trial for the efficacy and safety of lopinavir-ritonavir and
interferon-alpha 2b in hospitalization patients with novel coronavirus
pneumonia (COVID-1... 阅读全帖 |
|
n*******s
发帖数: 216 | 3 "刹车液就是液压传动用的，能有什么高温？"
文科生？
打回去google
Brake fluid boiling point
Brake fluid vaporizing
Brake Fade
不懂车不要紧，不要出来丢人即可
http://en.wikipedia.org/wiki/Brake_fluid
[edit]Service and maintenance
Most automotive professionals agree that glycol-based brake fluid, (DOT 3, DOT 4, DOT 5.1) should be flushed, or
changed, every 1–2 years.[2] Many manufacturers also require periodic fluid changes to ensure reliability and safety.
Once installed, moisture diffuses into the fluid through brake hoses and rubber seals... 阅读全帖 |
|
g********d
发帖数: 4174 | 4 Scientists in Denmark are reporting that they are just months away from
unveiling a breakthrough treatment that could functionally cure HIV, reports
U.K. newspaper the Telegraph.
The researchers are currently conducting clinical trials of a "novel
strategy" that strips the HIV virus from human DNA, allowing the immune
system to permanently destroy the virus. The treatment involves releasing
the HIV virus from "reservoirs" it forms within DNA, bringing the virus to
the surface of the cell. Resear... 阅读全帖 |
|
m*****n
发帖数: 9 | 5 这种靠颜色判断compatability的方法早过时了。其实前边几楼的已经说了一种可能就
是air trapped in the cooling system. need to bleed. One generic way is to
remove the radiator cap and let the car idle until the cooling fan kicks in.
The coolant will gush out and air will escape. Then turn off the engine and
fill the coolant to the top.你不bleed，开几个cycle，air也会慢慢跑出去。
现在的coolant的base其实大多都是ethylene glycol base。加了不同的dye和
proprietary corrosion inhibitor。德车的european coolant就是要避免phosphate，
而asian coolant的inhibitor有phosphate。现在的universal为了comp... 阅读全帖 |
|
c*****n
发帖数: 949 | 6 RECOMMENDATIONS — The following recommendations are based upon our clinical
practice given the existing evidence on therapies for dementia:
* Start patients with mild to moderate dementia on a cholinesterase
inhibitor. We do not routinely use tacrine. The choice between donepezil,
rivastigmine, and galantamine can be based upon cost, individual patient
tolerance, and physician experience, as efficacy appears to be similar. The
cholinesterase inhibitors offer potential symptomatic benefit tha |
|
f******I
发帖数: 769 | 7
when
most
just looked up, lacipil is a calcium channel blocker that is not available
in the states, if you are concerned of the lopressor's effect or side
effects, you can add another blood pressure meds, either a diuretic like
HCTZ, or the currently popular drug of choice an ACE inhibitor,
HCTZ reduce BP well but might also lower potassium and sodium levels and
makes youpee quite often; ace inhibitor could have a bothersome cough and
might cause angioedema, but is good for people with heart fa |
|
l*****O
发帖数: 402 | 8 if she used tamoxifen before, then she could use aromatase inhibitors now
given she is postmenopausal. Aromatase inhibitors usually doesn't work for
premenopausal women. How old was your mom when she's diagnosed? |
|
I****a
发帖数: 407 | 9 ER or PR (+) means tumor's growth some how is dependent on estrogen and it
is a good predictor as one can give either Tamoxifen or aromatase inhibitor
to block the estrogen. Her2/neu + traditionally means that the tumor is more
aggressive and hard to treat. Since Her2/neu monoclone antibody (herceptin)
is introduced, Her2/neu positivity is not that important because we now can
block the her2 pathway with herceptin and even with small tyrosine kinase
inhibitor such as Tykerb.
Overall for your mom... 阅读全帖 |
|
t****o
发帖数: 41 | 10 刚问清楚了，从2004年开始先是吃了1年多的“法乐通”，英文名是Fareston，药名是
Toremifene，然后因为子宫内膜增厚，又2006的时候做摘除胆囊手术，所以停了一年。
之后吃“弗隆”Femara，来曲唑，Letrozole，吃了2年多的样子吧，后来因为浑身发类
似湿疹的皮肤病，又换成了“瑞宁得”，阿那曲唑片，Anastrozole Tablets。这里“
法乐通”不知道是属于哪类药，似乎是比Tamoxifen好些。后面两个Femara和瑞宁得都
是属于aromatase inhibitor。
另外2004年的诊断和治疗是：
右乳癌，已行改良根治手术。术中病理报告：右乳腺导管内癌（多灶性），基地阴性，
巴结22个全部阴性。化疗5次，分别是： 第一次THP80mg，泰素210mg，第二，三，四
CTX0.8d，THP90mgd，第五次泰素210mgd.
inhibitor
more
herceptin)
can
treatment |
|
W***M
发帖数: 50 | 11 CML is very treatable since the development of tyrosine kinase inhibitor (
Imatinib). 5 year overall survival increased from 10-20% to 60-70% with TKI.
It is totally worth the money. the key is to take the medicine as long as
it still works. Sometime, CML can be resistant to imatinib, they you can
switch to second generation inhibitor such as dasatinib. |
|
f****o
发帖数: 2770 | 12 POSSIBLY EFFECTIVE
Age-related memory impairment. Taking ginkgo leaf extract orally seems to
improve cognitive function in some elderly people with mild to moderate age-
related memory or cognitive impairment. Ginkgo leaf extract might modestly
improve some measures of cognitive function, particularly short-term visual
memory and possibly speed of cognitive processing, in non-demented patients
with age-related memory impairment (5717,6216). But taking ginkgo leaf
extract does not improve memory ... 阅读全帖 |
|
l*h
发帖数: 4124 | 13 to be more specific, at this time, if you don't want to go the palliative
path, you should urge your dad's oncologist to consider the following
regimens:
1. a microtubule inhibitor + a platinum derivative or
2. a microtubule inhibitor + a platinum derivative + a conventional
alkylating agent
folate antimetabolites can also be considered but I would not place them
ahead of the above two options.
you should be very cautious to consider bevacizumab. remember, your dad is ~
65 yo. bevacizumab just c... 阅读全帖 |
|
l*h
发帖数: 4124 | 14 In principle, I agree with you on the use of pathway inhibitors. I think I
disagree with you on the practical issues.
1. her dad's oncologist doesn't seem to have a good knowledge of the
mutations of the tumor, the complexity of the pathways and the mechanisms of
these inhibitors.
2. his intracranial metastasis is a real big concern. if you think those
more conventional drugs are bad in getting into the brain (and the tumor),
currently available TKIs are worse. certain chemo drugs have been show... 阅读全帖 |
|
l*h
发帖数: 4124 | 15 Ponatinib is a new drug approved for patients with Ph+ ALL. Preliminary
results show it is superior to other tyrosine kinase inhibitors in Ph+ ALL.
It should not be available in China now.
Imatinib, dasatinib etc should be easier to obtain.
Dose scaling in pediatric patients of tyrosine kinase inhibitors: should be
based on body surface area, not on body weight.
L |
|
l*h
发帖数: 4124 | 16 Ponatinib is a new drug approved for patients with Ph+ ALL. Preliminary
results show it is superior to other tyrosine kinase inhibitors in Ph+ ALL.
It should not be available in China now.
Imatinib, dasatinib etc should be easier to obtain.
Dose scaling in pediatric patients of tyrosine kinase inhibitors: should be
based on body surface area, not on body weight.
L |
|
f****o
发帖数: 2770 | 17 国内先测EGFR, ALK,哪个positive就用哪个inhibitor。都是negative化疗。non small
cell lung cancer测的就是这两个基因突变。
PD1 inhibitor作为一线药物还没有批呢，虽然说也快批了
你爸爸都转移了，赶紧治疗吧。你来美国没有保险治疗起来你也是倾家荡产的 |
|
c*******n
发帖数: 892 | 18 Treatment
The goals of treatment are to relieve symptoms, prevent complications, and
delay kidney damage. To control nephrotic syndrome, you must treat the
disorder that is causing it. You may need treatment for life.
Treatments:
•Keep blood pressure at or below 130/80 mmHg to delay kidney damage.
Angiotensin-converting enzyme (ACE) inhibitors or angiotensin receptor
blockers (ARBs) are the medicines most often used. ACE inhibitors may also
help decrease the amount of protein lost in the u... 阅读全帖 |
|
y*******g
发帖数: 4 | 19 Postdoctoral Research Fellow Position in Institute of Glycomics, Griffith
University, Gold Coast, Australia
Prof Yaoqi Zhou’s group http://sparks-lab.org is built up a strong term for his molecular biology laboratory to interact with his computational laboratory at the Institute for Glycomics, Griffith University, Gold Coast, Australia. Initial exciting discovery in protein expression optimisation, antibiotic inhibitor discovery and protein design leads to the current expansion of the team. We... 阅读全帖 |
|
q*d
发帖数: 22178 | 20 ☆─────────────────────────────────────☆
swjtuer (swjtuer) 于 (Tue Mar 30 23:56:30 2010, 美东) 提到:
星期一
早餐 牛奶，煮鸡蛋，玉米馒头，榨菜
早点 芦柑
午餐 中大：绿豆饭，木须肉，海米冬瓜，棒骨菠菜汤
小婴：绿豆饭，三叶瓜炒肉，海米冬瓜，棒骨菠菜汤
午点 冰糖山楂水，蔬菜饼
晚餐 棒骨冬苋菜瘦肉粥，卤肉秋叶夹馍
星期二
早餐 牛奶，蒸鸡蛋，枣泥糕
早点 香蕉
午餐 中大：胡萝卜饭，芹菜炒牛肉，珊瑚豆腐，棒骨凤尾汤
小婴：胡萝卜饭，芹菜炒肉，珊瑚豆腐，棒骨凤尾汤
午点 果汁，卤猪肝
晚餐 豌豆肉末焖饭，番茄虾皮紫菜汤
星期三
早餐 南瓜粥，卤蛋，如意肉卷
早点 脐橙
午餐 中大：金银饭，咸烧白，海米儿菜，棒骨冬苋菜汤
小婴：金银饭，樱桃肉，海米儿菜，棒骨冬苋菜汤
午点 牛奶，蛋卷
晚餐 米饭，蒜苔炒肉，芙蓉蒸蛋，棒骨海带萝卜汤
星期四
早餐 牛奶，蒸鸡蛋，奶香馒头
早点 苹果
午餐 中大：南瓜饭，什锦鱼丸，韭菜炒豆干，番茄菌类鱼汤
... 阅读全帖 |
|
j***u
发帖数: 836 | 21 No. GILD's Sovaldi is part of it.
The news is on 8月27日. If it had been bad news for GILD, GILD price would
have already been hit down.
Not a bad news.
From many comments that I have read lately, for HVC market, the bigger
threat would come from MRK, but in 2H 2015 or later.
http://seekingalpha.com/article/2451415-gilead-earnings-forecas
"Regarding Hepatitis C, concerns have been raised regarding the market size,
competitors and patent vulnerability. I believe that it is now understood
that this ... 阅读全帖 |
|
M********s
发帖数: 104 | 22 以前网上淘到的一个,
College of _____
Department of __________
XXX ____ Avenue, 7th Floor
(city), (State) (zip) (推荐人地址)
August 12, 2007
U.S. Citizenship & Immigration Services
Nebraska Service Center
850 “S” Street Lincoln, NE 68508
Re: Dr. ____ ___ (your full name)
Dear Sir/Madam,
I strongly support the National Interest Waiver application for ______ ___,
M.D, Ph.D.. Dr. ___ is an extraordinary young scientist who is making
significant contributions to our understanding of ... 阅读全帖 |
|
s******8
发帖数: 77 | 23 在写petition letter中，google发现很多意外的东西。比如发表文章中发现了新的
inhibitor,就有商业公司合成和卖这个inhibitor，产品介绍中reference有引用文章。
这种应该怎么claim对自己有利？ 不太想去专门找推荐信，时间来不及。如果只是打
印公司网页是不是不够solid？多谢指教！ |
|
f********6
发帖数: 195 | 24 TITLE: The comparision of the Wnt signalling pathway inhibitor delivered
electrospun nanoyarn fabricated with two methods for the application of
urethroplasty
Journal of Materials Chemistry B
ABSTRACT:
Urethral stricture is a common disease caused by over-expression of
extracellular matrix from fibroblast. However, the fibroblasts is a main
type of cells for the repair of urethra.Here, we compared two nanoyarn
scaffolds with the characteristics for improving fibroblasts infiltration
but inhibiti... 阅读全帖 |
|
s*********k
发帖数: 1989 | 25 Lipitor is to expire soon. There are new ones coming still working at
inhibitor.
Just curious about any alternatives.
There is bio-synthesis, but there is almost no way to change the bio-
synthesis except inhibitor in-take.
All foods have the cholesterol source, but it cannot hit each cell since
they
must be digusted forehand.
Personal curiousity.
synthesis |
|
a*****y
发帖数: 269 | 26 Please send in cover letter and CV to Nathan Lee: n**********[email protected]
Postdoctoral Positions in Pfizer Oncology, La Jolla CA
The post doctoral positions will be responsible for elucidating the
mechanism of action of existing Pfizer
portfolio compounds, ensuring that we have the appropriate patient selection
strategies for such
mechanisms, aligning the research into patient selection with the
appropriate diagnostic assay,
understanding mechanisms by which patients fail (or become resistant to... 阅读全帖 |
|
m******j
发帖数: 5079 | 27 http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0001911/
Symptoms
Early breast cancer usually does not cause symptoms. This is why regular
breast exams are important. As the cancer grows, symptoms may include:
Breast lump or lump in the armpit that is hard, has uneven edges, and
usually does not hurt
Change in the size, shape, or feel of the breast or nipple -- for example,
you may have redness, dimpling, or puckering that looks like the skin of an
orange
Fluid coming from the nipple -- may be blood... 阅读全帖 |
|
K****o
发帖数: 2183 | 28 据我有限的知识，CI injection在很多subsea production system里都需要，用来保护
pipeline等等内壁。我不是这个方向的内行，不过你入了这个方向，应该能接触到相关
的asphaltene inhibitor, scale inhibitor等等，可以以后在upstream走production
chemistry方向 |
|
G***a
发帖数: 27294 | 29 首先，豆浆（黄豆）里面有的不是蛋白酶，是蛋白酶抑制剂。确切地说，是胰蛋白酶抑
制剂
即soybean trypsin inhibitors
其次，绝大多数实验证明，soybean trypsin inhibitors对人体胰腺蛋白酶没有任何有
害作用
甚至有人说它将来可以用于胰腺癌症的研究。
最后，即便有害，这个东西，加热就能完全灭活。实在害怕，把豆浆煮开了喝，大分子
都变性解旋了
怎么能刺激肠道壁粘膜呢？
具体参见以下文献
http://www.ncbi.nlm.nih.gov/pubmed/7036513 |
|
v******s
发帖数: 6949 | 30 http://en.wikipedia.org/wiki/Antinutrient
Phytic acid has a strong binding affinity to minerals such as calcium,
magnesium, iron, copper, and zinc. This results in precipitation, making the
minerals unavailable for absorption in the intestines.Phytic acids are
common in the hulls of nuts, seeds and grains.
Protease inhibitors are substances that inhibit the actions of trypsin,
pepsin and other proteases in the gut, preventing the digestion and
subsequent absorption of protein. For example, Bowma... 阅读全帖 |
|
b***u
发帖数: 12010 | 31 http://half-life.wikia.com/wiki/Aperture_Science
Aperture Science, Inc.,[7] often simply referred to as Aperture Science or
simply Aperture, is an American scientific research corporation whose
laboratories and administration, Aperture Laboratories, are located in
Cleveland, Ohio, USA.[1] Originally a shower curtain manufacturer, it
evolved over the course of half a century into an experimental physics
research institution and a bitter rival of Black Mesa.
"Aperture Laboratories" is also used as... 阅读全帖 |
|
B*****I
发帖数: 1378 | 32 就是简单的mma，3MBP,crosslinker
用个prepacked column除inhibitor
可是看了一下那个column颗粒非常大，流得非常快，有点担心是不是没有除干净
inhibitor
用很强的光照了一个小时才polymerize了，似乎太慢了。。是不是?
3MBP用了最多有4%,也没用
啥问题？换柱子么？ |
|
发帖数: 1 | 33 【 以下文字转载自 Comment 俱乐部 】
发信人: CornucopiaX (小土豆), 信区: Comment
标 题: Trazodone
发信站: BBS 未名空间站 (Wed Jun 21 20:35:15 2017, 美东)
Trazodone (sold under the brand name Oleptro, among many others)[1] is an
antidepressant of the serotonin antagonist and reuptake inhibitor (SARI)
class. It is a phenylpiperazine compound. Trazodone also has anti-anxiety (
anxiolytic) and sleep-inducing (hypnotic) effects.[5] Its side-effect
profile and potential toxicity are considerably different from those of the
original ... 阅读全帖 |
|
F****n
发帖数: 438 | 34 【 以下文字转载自 Medicalpractice 讨论区 】
发信人: FeiLun (Is Horrible), 信区: Medicalpractice
标 题: 几篇期刊文章
发信站: BBS 未名空间站 (Sat Jul 12 22:36:30 2014, 美东)
那位大侠可以帮组查一下这几篇的全文吗？叩谢。
请发：y******[email protected].
I.
Role of small-conductance calcium-activated potassium channels in atrial
electrophysiology and fibrillation in the dog.
Qi XY1, Diness JG, Brundel BJ, Zhou XB, Naud P, Wu CT, Huang H, Harada M,
Aflaki M, Dobrev D, Grunnet M, Nattel S.
Author information
Abstract
BACKGROUND:
Recent evidence points to functional... 阅读全帖 |
|
c*****t
发帖数: 1084 | 35 这么说吧，你这个问题简直就没边了。这种表达量降低是普遍现象还
是这个启动子特异的？你可以测一下其他基因的表达水平， 或者做
internal control。 This way you'll know whether it's specific
for your promoter or just general effect by the drug.
secondly,what kind of drug do you use? transcription inhibitor?
translation inhibitor or anything else?
if it's specific for your promoter you can do RPA or northern
to determine whether the RNA level changes. and you can do
nuclear runon to test whether the transcription rate is affected.
or your drug triggers some RNA d |
|
s******y
发帖数: 28562 | 36 This is a convenient recipe: 0.5% Triton X-100, 20~50mM Hepes-KOH, pH 7.8,
50mM KCl, 2mM MgCl2, 5mM EGTA,1mM EDTA, 100mM Sucrose, Plus cocktail of
protease inhibitors.
Triton X-100 is for lysis of cell membrane, Sucrose is for the stablity of
most proteins, protease inhibitors is again proteolysis.
However, this may differe depend on your purpose. You should check related
reference to get a recipe that works for your experiment
By the way, in some recipe, I notice they don't use Triton, don't un |
|
j*****a
发帖数: 658 | 37 1。 proteinase inhibitor cocktail and phosphatase inhibitors 是要加的。他们
的作用是防止你在抽提蛋白的过程中蛋白讲解。在你一开始用pbs悬细胞的时候，如果
不加这两种抑制剂，会有蛋白降解。一般我洗细胞的时候就开始加。当然蛋白煮过变性
就不存在这个问题了。
2。 你的方法当然是可行的。5x sds page loading buffer 里就有detergent SDS，然
后再加热裂解，当然没错。如果你对自己的手平行操作非常confident，是完全可以不
测蛋白浓度的，直接加热完后run gel。 其实测蛋白的时候如果做的好一般情况下，
sample和sample之间浓度都是差不多的，校正和不校正是没有很大差的。所以你的做法
没有啥不对。只是你老板的做法是小学生式的，step by step, 也是classic的做法。
3. RIPA buffer是很强的裂解液，里面有几种detergent 组合，所以一般情况下裂解是
很完全的。就像上面的人说的，超声主要是打碎DNA，有点牛刀杀鸡了。
4。关于loading contro |
|
h**2
发帖数: 2841 | 38 老弟/老妹，很多实验室都有自己的习惯，尤其是你做phd的话人家assume你是张白纸，
谦虚小心点follow他们的习惯总不会错。
虽然你的这个实验beta actin是匀的，但对于你的这个方法对establish自己的实验系
统很不好。
首先以后你可能会碰到细胞数在transfection/infection之后变化很多，其次你用pbs
冲下来的话也可能漏掉一些，这些都是很可能的，实验多了的话难免出错，但你最快也
要等到running/transferring之后用ponceau S才能知道，浪费时间啊。至于裂解的话
找个标准的protocol用RIPA来做吧，算是经典方法之一，不想配的话公司有卖的。记得
加上protease inhibitor和phoshpatase inhibitor (thermo)也有卖的
我老板平时都不管学生，但每个新生进实验室一般迟早都会被骂一次，算是整个phd培
养过程唯一一次micro-management，常常会说it's scientific research, not
cooking!开始也有点不服气，但后来慢慢意识到每一步仔细一点，其实整体上 |
|
l*h
发帖数: 4124 | 39 There is no clinically available migration/metastasis inhibitor.
There are quite some research efforts trying to figure out what causes
cancer cells to metastasize. No real answer has been found yet (A lot of
molecules are involved.) For research, as the last time I did a literature
survey, there is no cancer cell (even for a specific cancer type) specific
migration inhibitor.
metatasis, |
|
y**s
发帖数: 6809 | 40 弱弱的问一下，发育完成之后，成体的哪些细胞需要migrate啊？
There is no clinically available migration/metastasis inhibitor.
There are quite some research efforts trying to figure out what causes
cancer cells to metastasize. No real answer has been found yet (A lot of
molecules are involved.) For research, as the last time I did a literature
survey, there is no cancer cell (even for a specific cancer type) specific
migration inhibitor.
metatasis, |
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c****y
发帖数: 14 | 41 各位大侠：
我想用药物Cisplatin, 或者 Etoposide (DNA topoisomerase II inhibitor), 或
者camptothecin (topoisomerase I inhibitor)处理细胞诱导DNA damage，请问该选择
哪些蛋白做Marker证明药物处理确实诱导了DNA damage. 谢谢帮助！ |
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C******r
发帖数: 790 | 42 我也是约来越不会做western了
在以前实验室，从没做过的三四个内源蛋白CoIP，一次就能做出来
现在实验室，这次有的信号（doublet磷酸化band）下次就没有了（只有脏背景，没有
条带），搞得我每次去显影都提心吊胆
即使cell line相同，抗体相同，稀释倍数相同，一个人western跟另一个人western不
一样的可能原因太多了，试着列举一下：
1、cell lysis buffer的离子强度和去污剂强度不同，裂解时间长短不同，导致你们
lysate里的蛋白种量有差异；至于要看磷酸化band的话，裂解液是否加phosphotate
inhibitor, 加的种类，也超级影响结果。我不加phosphotase inhibitor的话，有时候
可以看到band shift，但更多时候看不到。
2、SDS胶的浓度，Tris的浓度和pH；这个有人说有影响，我还没有比较过。
3、转膜的电流、时间、温度，以及转膜液里的离子强度（Tris和Glycine的量）,以及
是否加有SDS，都会影响转膜效果。个别抗体对转膜有没有SDS会很敏感，没有哪个配方
是绝对的好。
4、一抗和二抗的稀释倍... 阅读全帖 |
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a*****y
发帖数: 269 | 43 【 以下文字转载自 SanDiego 讨论区 】
发信人: alvinpy (生命就是一场歪打正着), 信区: SanDiego
标 题: Post-Doc Positions at Pfizer
发信站: BBS 未名空间站 (Tue Nov 16 20:10:46 2010, 美东)
Please send in cover letter and CV to Nathan Lee: n**********[email protected]
Postdoctoral Positions in Pfizer Oncology, La Jolla CA
The post doctoral positions will be responsible for elucidating the
mechanism of action of existing Pfizer
portfolio compounds, ensuring that we have the appropriate patient selection
strategies for such
mechanisms, aligning ... 阅读全帖 |
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O******e
发帖数: 4845 | 44 真正的单基因疾病很少很少，我们做了这么多年的应该清楚，很多文章宣称的哪个基因
突变
导致哪个疾病，其实证据非常不足。
再往大了说，很多疾病不是简单的“基因"或“遗传”问题，牵扯到太多的因素。而
RNAi
is designed to target only one or a few genes.
这个跟kinase inhibitor drugs区别还是很大，因为好的inhibitor只是抑制部分功能
；而
knock-down是把整体的蛋白水平降低，这个的影响会非常广泛和难以控制。 |
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L******e
发帖数: 679 | 45 将会是肿瘤治疗的下一个‘泡泡’吗？
最近好像很火：
1,借着next generation sequencer' 的东风，大家都在疯狂的寻找‘dirver
mutations'.
2,制药公司在大量的筛选'small molecule inhibitors',这些小分子可以抑制突变了的
kinase,从而阻断肿瘤细胞的生长。
3, 前段时间的plx-4032，BRAF v600E 的inhibitor,80%有这个基因突变的皮肤癌病人
有效。
但是，几乎毫无列外，大概一年后肿瘤细胞会产生第二个突变，获得耐药性。
小弟刚刚接触这个行业，有大拿能展望一下吗？
另外，搞基础研究的大虾能不能提供一些关于小分子诱导基因突变机制的文章或者综述
。不是那种简单的化学物质能把某个基团变成另外基团的解释。
因为耐药性的产生不是随机的：例如肺癌病人对erlotinib的耐药性，50% 的病人是在
EGFR上产生第二个突变T790M,这个突变改变了erlotinib对EGFR 的亲和力。这中间肯定
存在调控，使有目的性的突变。 |
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d*****r
发帖数: 2583 | 46 ☆─────────────────────────────────────☆
timeonly (圆满) 于 (Thu Dec 23 15:28:05 2010, 美东) 提到:
发信人: desesperado (Estoy), 信区: Military
标 题: 方舟子谈蜂蜜：基本上是高浓度的糖
发信站: BBS 未名空间站 (Thu Dec 23 12:11:10 2010, 美东)
http://discover.news.163.com/10/1223/10/6OJ4ABLE000125LI.html
核心提示：方舟子认为用人工的方法，可以制造出一种与蜂蜜一模一样成分的东西。其实蜂蜜并不神秘，基本上就是高度浓缩的糖。蜂蜜除了水分，剩下的几乎都是各种各样的糖。蜂蜜的成分会有变化，但大同小异。
中青在线-中国青年报12月23日报道 什么是蜂蜜呢？这个问题似乎很简单，是蜜蜂采集花蜜酿造出来的甜味物质。蜜蜂把花蜜吸到它的第二个胃 ——“蜜胃”里面，在那里进行消化（让花蜜中的蔗糖大部分转化成果糖和葡萄糖），回到蜂巢吐出来，让水分蒸发、浓缩，这样，原来
含水分大约 80%）... 阅读全帖 |
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S*****s
发帖数: 287 | 47 我想搞清楚一个在细胞质内的突变蛋白是怎么被降解的。这个蛋白的野生型有两种
alternative intron splicing 的 product，姑且叫 wildtype 和 wildtype2 吧。我
在 293 细胞内表达两个 wildtype 和 mutant 蛋白，然后用 proteosome inhibitor
（Lactacystin) 和 lysosome inhibitor （Chloroquine, Leupeptin) 来处理细胞。
实验结果发现 Lactacystin 处理之后 wildtype 蛋白含量不变，wildtype2 含量升高
，但是 mutant 蛋白含量下降了。Chloroquine 和 Leupeptin 处理之后也不见 mutant
蛋白含量升高。Excel 的分析图也随帖附上。这样我就搞不明白这个蛋白是被什么降
解的了。请问版上的各位大牛能不能指点一下，下一步应该从什么角度去研究蛋白降解
？先谢谢大家了！ |
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w********y
发帖数: 288 | 48 不用上网搜，我自己就有实验证据。我之前做TA带过一回本科生理实验，有一个实验测
rats的代谢效率，用的三组动物模型就是这么做的，一组ctrl，一组低代谢事先给注射
一个什么inhibitor——好象是I3，I4转化那个酶的inhibitor，记不清了，另一组高代
谢的就是提前一星期给动物吃碘，最后各种生理指标发现代谢水平提高。
碘确实能够提高代谢效率，所以碘片不能像钙片一样随便吃，都是给需要的病人吃的。
对于防辐射，我只是不清楚提到代谢效率是不是能够帮助把受辐射损伤的组织排出体外
，所以问问大家讨论一下。 |
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q*****n
发帖数: 331 | 49 For mouse experiment, you are going to need large quantity of antibodies or
small kinase inhibitors. It is unrealistic to buy them from bio-reagent
company.
what your boss can do is to set up collaborations with big people working in
the IGF field who has the antibody or inhibitor, or collaborate with
pharmaceutical company. For example:
OSI: OSI-906
BMS: BMS-754807
You can search pubmed to find references for these two drugs and author
information.
Good luck! |
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j****x
发帖数: 1704 | 50 有没有人在做最针对这些个疑似receptor的药物，我也不是很清楚，只知道现在号称的
那些个receptor，没有一个是真正HBV specific甚至hepatocyte specific的，所以我
猜测这些不会是很好的target。PreS1 derived peptide作为entry inhibitor目前据说
效果已经非常好了，另有其他一些entry inhibitory peptide/molecule在pre-
clinical阶段的效果也不错，所以我估计眼下针对“疑似”receptor的rational
design不会有太大的吸引力。
chronic HBV治疗，要说最终解决还是得靠免疫学的方法，传统的NRTI也好，entry
inhibitor也罢，治标不治本，解决不了问题。强效Interferon以及前几天热炒的
Adoptive T cell therapy，我觉得应该是目前看来最有前景的方向吧，这玩意在国内
时市场太大了。
是了
用的 |
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