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a*i
发帖数: 377 | 2 说实话是因为看见老鼠两字就觉得恶心
写成mice感觉好点
但是写到这句的时候确实露怯了
请问这个到底普遍不普遍啊 |
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t*****l
发帖数: 48 | 3 I am a postdoc working in Mayo Clinic, working on structures of proteins
involved in Cancer development right now , and we work on mice, too. I am
eagely looking for review opportunities. My email address is dongmeijiao@
gmail.com. Thank you very much. |
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E****l
发帖数: 577 | 4 看了个townhouse，觉得各方面还算满意，想出个offer(最晚明天了)，就是TDS上有一
句Property has been treated for mice problem.房子是crawl foundation的。
想请教下大伙，这鼠患的问题，清理了以后会复发么？（特别是townhouse，估计和邻
居家都是户户相连，老鼠会串门吧～）
有过老鼠的房子还能买么？哪位有这方面的经验分享一下！
谢谢先了！ |
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g****g
发帖数: 45 | 5 午饭时候有个哥大生物系的教授一起吃，我们闲聊。我问：你们做实验用rat吗？
这位教授顿了一下，盯着我说：我们用mice。 |
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w****8
发帖数: 36 | 6 and never cross the mice both have the cre allel, because then you cannot
distinguish the homo and hetero of
the cre line. |
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h*******e
发帖数: 205 | 7 那在中国还没有进行类似的实验为什么就开始种植了？
岂不是更说不通？
意大利人和中国人之间的差异肯定要比老鼠和人之间的差异要小的多。
system
MON
mice
pellet |
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l*******6
发帖数: 90 | 8 要做transgenic mice
想只在大脑中表达，用什么样的启动子好?
推荐一个商业化或容易得到载体质粒载体
以前从一个Lab要过一个pNSE-EX4的质粒，可转化后菌都张不出来
这次我想直接从公司买
那么老大能给点信息或指点一下 |
|
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a******m
发帖数: 6 | 9 Hi everyone,
I need genomic DNA/small piece of tissues from 129X1/SvJ mice as controls.
Is there anyone can help me?
Many thanks in advance. |
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a*******g
发帖数: 162 | 10 想GENERATE HOMOZYGOTE的TG MICE， 请问怎么可以GENOTYPING啊，用QPCR可靠吗？需
要用TAQMAN还是普通QPCR就可以了？多谢！ |
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S*****s
发帖数: 287 | 11 这个难度比较高。你知道 transgene 插入在基因组的位置么？如果知道的话设计
Primer 扩充相应位置就行了。好奇的问一句，你为什么要 homozygous tg mice？ |
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f******9
发帖数: 39 | 12 最近看文献，看到C47BL/6和C57BL/6 mice。
谁知道这两个strain的起源有何不同？是不是分别来自C47号和C57号老鼠啊？
多谢。 |
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D*****l
发帖数: 554 | 13 我最近得到knockout mice.一个可能是癌抑制基因，被我成功knockout.小鼠有三个月
大，但没有任何表现型。
一次偶然机会，我发现，和对照比较，p53只有5%表达量在knockout小鼠中。
我的老板是个MD, 随我玩试验，只要有与癌相关的结果就行！请各位大牛出出高招，能
弄个好文章！ |
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O******e
发帖数: 4845 | 14 如果我是你，第一要做的，就是确定这个5% p53 expression。
你得非常肯定这个变化是真的，否则可能是瞎忙活。
第二要做的，比较快的，give them some DNA damage or other tumorigenic
drugs/chemicals，然后观察肿瘤发生的变化。
如果你那个5%的表达量是真的，什么都不做，你的KO Mice也会自然产生肿瘤并很快死
掉。 |
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h********n
发帖数: 4079 | 15 This is why MMHCC exists.
My phd boss always give mice to others when asked. |
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b*******0
发帖数: 48 | 16 "Hemizygous transgenic mice are viable, fertile, normal in size and do not
display any gross physical or behavioral abnormalities."
我在想是不是我跑pcr的时候，没有加internal positive control primer的原因，当
初就加了transgene 的primer, 觉得那个control加不加无所谓。会不会那个也有影响
呢，导致本来该出现的band,没有出现。 |
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v***a
发帖数: 1242 | 17 我从来没有接触过KO mice
可我目前得到的结果是，超量表达此蛋白后对机体影响特别大（in vivo，在我们的
system中）
所以觉得很难办。。。 |
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c*********7
发帖数: 148 | 18 那些KO mice 有没有被challenge过？ |
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f********1
发帖数: 367 | 19 在看一篇文献，其中用到bone marrow chimeric mice，有些不懂的地方。
比如，这篇文章里面用的recepient是proteinA-/-的鼠，用致死剂量的辐射处理后，接
受来自protein A +/+的鼠的donor cell。文章里面讲到，在这样产生的鼠里面，CNS
resident microglia是proteinA-/-，我的理解是，CNS里面不光是microglia，其他类
型的细胞也是proteinA-/-，这样的话，用这个方法来证明protein A是通过microglia作用的是不够有说服力的。不知道这样理解对吗？
恳请内行指教，谢谢先！ |
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s******a
发帖数: 472 | 20 I have ordered BACs and done recombineering to obtain modified insert
flanked by homologous arms. The plan is to further introduce a selection
cassette (neo), after which modified insert flanked by homologous arms will
be retrieved into targeting vector with negative selection marker.
And the next step will be ES targeting and transgenic mice generation...But
that's quite far away. Now I am just planning the strategies which I can use
to screen ES clones for correctly targeted insert.
PS, the bo... 阅读全帖 |
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m******5
发帖数: 1383 | 21 in-proportional advantage when you are to try to mate your chimera mice. |
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p*********e
发帖数: 23 | 22 也有这种情况
但楼主说“是利用在其两个exons之间插入另一个不相关的gene，来使其原蛋白丧失功
能”
所以严重怀疑是个genetrap knockout
后来楼主又提到“此KO mice体内有好几种此蛋白的变异体（由不同的exons变换组合而
成）”
我想这是指alternative splicing
一般用同源重组做KO时，可以在设计时避免alternative splicing的干扰。 |
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G********e
发帖数: 528 | 23 Thank you very much!
It seems that if I want to bypass embryonic lethality, it is a candidate. If
people have been using tissue specific systems, they would be better than
whole body.
I have something in my mind before I want to start it.
If a gene of interest is known critical for embryonic development, is it
really important to bypass the embryonic stage and look at the phenotypes of
loss of function after the mice are fully developed? I think so, but it
seems not many people publish studies u... 阅读全帖 |
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w*e
发帖数: 740 | 24 it is not very complicated if your university has a good animal facility.
I have ordered mice from UK and Germany. |
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l****j
发帖数: 70 | 25 knockout的mice基因型检验没有问题，蛋白质却没变化，
是抗体还是knockout有问题? |
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m******5
发帖数: 1383 | 26 Dear All:
I got a mice by hand with specific genetic trait, I want to get a homozygote
mES cell line from it. Knowing that we could not isolate mES cell from
blastocyst in our facility, I tunred to iPS or nuclear transfer.
IS there known facility out there provide service that we could make iPS or
nuclear transfered ES cell in a reasonable price range? thanks!!! |
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a*****x
发帖数: 901 | 27 这篇文章开头就有的：
“We have shown previously that embryonic stem cells (ESCs) and induced
pluripotent stem cells (iPSCs) from male (XY) mice are induced into epiblast
-like cells (EpiLCs), which are in turn induced into primordial germ cell–
like cells (PGCLCs) with proper function for spermatogenesis and offspring
production”
所以精子用的是XY
oocyte |
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s****9
发帖数: 932 | 28 Just the mice directly from JAX or breeder from your own colony.
If direct from JAX, I would say it is the compensation issue.
Cre |
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f*******a
发帖数: 671 | 29 我准备买一个有荧光的reporter mice，和我的xxx-creERT2交配，在1~2个月的时候给
tamoxifen
,2个月的时候观察脑袋.但是听说有的不太敏感，太敏感的又不太特异。Jackson上面选
择太多了。有人能推荐一下吗？或者给个Jackson Lab的链接。
用ROSA在成鼠好像不太表达，所以不打算买这个。 |
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u***e
发帖数: 71 | 30 Should transgenic mice from the same founder have the equal copy number of
transgenes? Any idea? |
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m******t
发帖数: 109 | 31 大家有没有发现inbred mice很容易失掉phenotype 怎么解决呢 和WT CROSS 一下? |
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s****n
发帖数: 16 | 32 it is popular in flies and zebrafish. why not in mice? any reasons other
than convenience? |
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r*****i
发帖数: 117 | 33 should be in the same mouse. Otherwise, how can you exclude the viability
among mice. |
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c*****x
发帖数: 83 | 34 【 以下文字转载自 Immigration 讨论区 】
发信人: cookiex (checking。。棉花依旧), 信区: Immigration
标 题: review opportunity 两分左右杂志 HBV mice model
发信站: BBS 未名空间站 (Wed Dec 2 14:09:10 2015, 美东)
需要两位， 请私信 姓名 邮箱， 简短介绍 （背景最好match）
两分左右杂志。
希望能尽快完成review。
多谢 |
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r******g
发帖数: 600 | 35 integration site 不知道的话，文章一样发啊
90年代 做transgenic mice 很多都不知道loci的
你准备怎么cross 老鼠呢？ 讲来听听，我挺好奇的。我自己从来没遇到过这种情况
我想了想，觉得 先cross 出heterozygous A+/-. B+/- 然后 再跟WT cross
直接 同时PCR A和B, 得到double positive 为成功的haplotype。 貌似用这种办法，
screening strategy比较简单
lz准备怎么操作？纯属好奇
same
routine
kinds |
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V*******s
发帖数: 19 | 36 Hi: Anyone here works on ROSA26 construct to make transgenic mice?
Thanks, |
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g***j
发帖数: 40861 | 37 BPA exposure makes male deer mice less attractive to females
BIOLOGICAL SCIENCES
Latest research has implications for humans, other species
The latest research from the University of Missouri on bisphenol A shows
that the ubiquitous chemical causes male deer mice to become “
demasculinized,” leading scientists to conclude that exposure during human
development could be damaging to behavioral and cognitive traits that are
important in reproduction.
The MU research demonstrated a link between bisp... 阅读全帖 |
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M********e
发帖数: 2528 | 38 我不知道你是从哪里来的概念，比如“超过3个月以上喂养的实验概不算数？”，比如
科学实验从来不test超过3个月什么的，你是否读过任何文章啊？
下面是引用的综述的文章。
http://www.sciencedirect.com/science/article/pii/S0278691511006
在table3里列了很多研究转基因玉米及其他对动物的影响。在这个就有好几年研究啊。
这些研究结果都没发现转基因比传统育种的更risky。
不过我觉得我的确太闲了。
Table 3.
Impact of GM plant diets in multigenerational studies.
Plant Trait Reference & Funding Species Duration Parameters
Group (n = number of individuals per group) Mai... 阅读全帖 |
|
发帖数: 1 | 39 07 Nov 2019
Just as the clamor about Biogen’s seeking FDA approval for aducanumab with
one positive Phase 3 trial started to die down, a Chinese company garnered a
regulatory thumbs-up for its drug, also with one Phase 3 trial. On November
2, Shanghai Green Valley Pharmaceuticals announced that China’s National
Medical Product Administration (NMPA) had conditionally approved GV-971, aka
oligomannate, for the treatment of mild to moderate Alzheimer’s disease.
Full approval, expected to come in sp... 阅读全帖 |
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i*****s
发帖数: 4596 | 40 http://www.nytimes.com/2013/02/15/science/studying-recent-human
East Asian Physical Traits Linked to 35,000-Year-Old Mutation
By NICHOLAS WADE
Published: February 14, 2013
Gaining a deep insight into human evolution, researchers have identified a
mutation in a critical human gene as the source of several distinctive
traits that make East Asians different from other races.
The traits — thicker hair shafts, more sweat glands, characteristically
identified teeth and smaller breasts — are the re... 阅读全帖 |
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p**********u
发帖数: 15479 | 41 【 以下文字转载自 WaterWorld 讨论区 】
发信人: ldy (现在我很害怕，可能时间会炸), 信区: WaterWorld
标 题: 东亚女胸小的原因找到了
发信站: BBS 未名空间站 (Fri Feb 15 11:11:04 2013, 美东)
发信人: inergas (大坏猪), 信区: Military
标 题: 东亚女胸小的原因找到了
发信站: BBS 未名空间站 (Fri Feb 15 09:21:19 2013, 美东)
http://www.nytimes.com/2013/02/15/science/studying-recent-human
East Asian Physical Traits Linked to 35,000-Year-Old Mutation
By NICHOLAS WADE
Published: February 14, 2013
Gaining a deep insight into human evolution, researchers have identified a
mutation in a critic... 阅读全帖 |
|
l*y
发帖数: 21010 | 42 【 以下文字转载自 Military 讨论区 】
发信人: inergas (大坏猪), 信区: Military
标 题: 东亚女胸小的原因找到了
发信站: BBS 未名空间站 (Fri Feb 15 09:21:19 2013, 美东)
http://www.nytimes.com/2013/02/15/science/studying-recent-human
East Asian Physical Traits Linked to 35,000-Year-Old Mutation
By NICHOLAS WADE
Published: February 14, 2013
Gaining a deep insight into human evolution, researchers have identified a
mutation in a critical human gene as the source of several distinctive
traits that make East Asians different from other races.... 阅读全帖 |
|
l*y
发帖数: 21010 | 43 【 以下文字转载自 Military 讨论区 】
发信人: inergas (大坏猪), 信区: Military
标 题: 东亚女胸小的原因找到了
发信站: BBS 未名空间站 (Fri Feb 15 09:21:19 2013, 美东)
http://www.nytimes.com/2013/02/15/science/studying-recent-human
East Asian Physical Traits Linked to 35,000-Year-Old Mutation
By NICHOLAS WADE
Published: February 14, 2013
Gaining a deep insight into human evolution, researchers have identified a
mutation in a critical human gene as the source of several distinctive
traits that make East Asians different from other races.... 阅读全帖 |
|
D*******8
发帖数: 4 | 44 Please don’t dig me out. I am writing this out of my conscience as a
scientist. We are using, largely, the tax payers’ money to explore
mechanisms underlying diseases that we don’t have a cure, yet. We are doing
this in the hope that little bit knowledge can accumulate till one day we
can find that magic bullet. However, there are people out there, their
purpose is to use these money to achieve their personal fames, sometimes
international ones, totally disregarding our original noble cause. I... 阅读全帖 |
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s******y
发帖数: 28562 | 45 Blind' mice eyesight treated with transplanted cells
Lead researcher, Prof Robin Ali , explains the behaviour of blind mice in
the water maze test. Video filmed and produced by UCL and Moorfields
Hospital
Related Stories
British scientists have restored the sight of blind mice by transplanting
light-sensitive photoreceptor cells into their eyes.
The work is a step towards a new treatment for patients with degenerative
eye diseases.
Scientists at University College London Institute of Ophthalmolo... 阅读全帖 |
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X*******8
发帖数: 3895 | 46 刚才看有人在“请教一个转基因问题”，故把下面一篇文章转过来，没有翻译，有兴趣
的自己看吧。
http://www.holistichealthsecrets.com/alternativehealth/genetica
modified-foods-healthy-to-eat/
Are Genetically Modified Foods Healthy to Eat?
July 14th, 2009adminLeave a commentGo to comments
The topic of Genetically Modified Food (GMOs) can be confusing. On one side
we have big Pharma
companies touting studies that show that Genetically Modified Food is
perfectly safe for consumption. On
the other hand, we have wholistic health groups and speakers s... 阅读全帖 |
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X*******8
发帖数: 3895 | 47 读读这个吧，没有翻译，有兴趣
的自己看吧。AAEM的人你不因该叫民科了吧？另外，谁把我关于先锋分类的贴删了？每
必要这么害怕吧？
http://www.holistichealthsecrets.com/alternativehealth/genetica
modified-foods-healthy-to-eat/
Are Genetically Modified Foods Healthy to Eat?
July 14th, 2009adminLeave a commentGo to comments
The topic of Genetically Modified Food (GMOs) can be confusing. On one side
we have big Pharma
companies touting studies that show that Genetically Modified Food is
perfectly safe for consumption. On
the other hand, we have wholistic health gr... 阅读全帖 |
|
发帖数: 1 | 48
Download pdf published by SfN (Chapter from 'The History of Neuroscience in
Autobiography, Volume 8' edited by Larry R. Squire)
Yuh-Nung Jan's CV
Lily Jan's CV
Yuh-Nung Jan and Lily Jan
Birth
Family History and Growing Up
National Taiwan University
The Hiking Trip to Shitou in the Spring of 1967
Graduate School Application
Graduate Study at Caltech (1968锟974)
Seymour Benzer Lab (1974锟977)
Steve Kuffler锟絪 Lab at H... 阅读全帖 |
|
