r******g
发帖数: 600 | 1 最近 对这个领域 稍微研究了一下，懂得不多，只是提提个人观点
其实你们两个人 说的是对的
你可能看过RP Lifton的papers。他一直致力于 研究 rare mendelian diseases。 他
目前和BCM,Wash U@SL 和 JHU 一起在做 mendelian genetics consortium。 通过在
全世界找 “extreme phenotype - disease outliers” 和 “inbred family的疾病”
来寻找 human knockout的phenotype。
通过 RP Lifton的实验，你发现，这个世界上 仍然有很多 monogenic的病。 但是从
临床的角度来考虑， 研究 monogenic的疾病 究竟有治疗价值吗？ 1/100,000 的
mutation导致的 rare disease, 有哪个药厂会愿意生产药物来治疗呢？ 所以，工业圈
自然也不会对这个领域感兴趣...
但是，你如果仔细听听RP lifton的讲座，你会发现 “发现rare disease其实并不是RP
lifton的目的”！！ 通过 研究 ext... 阅读全帖 |
|
b****r
发帖数: 17995 | 2
懂得不多，只是提提个人观点
其实你们两个人 说的是对的
你可能看过RP Lifton的papers。他一直致力于 研究 rare mendelian diseases。 他
目前和BCM,Wash U@SL 和 JHU 一起在做 mendelian genetics consortium。 通过在
全世界找 “extreme phenotype - disease outliers” 和 “inbred family的疾病”
来寻找 human knockout的phenotype。
通过 RP Lifton的实验，你发现，这个世界上 仍然有很多 monogenic的病。 但是从
临床的角度来考虑， 研究 monogenic的疾病 究竟有治疗价值吗？ 1/100,000 的
mutation导致的 rare disease, 有哪个药厂会愿意生产药物来治疗呢？ 所以，工业圈
自然也不会对这个领域感兴趣...
--你不能把眼球停留在眼前，应该放眼10年以后，你职业生涯的成熟期。100，000人一
例患者，全世界有多少患者？数十万！测序技术的普及以及互联网，在几年后就会把这
几十万人基本... 阅读全帖 |
|
yf
发帖数: 272 | 3 http://jop.ascopubs.org/content/early/2013/03/26/JOP.2012.00081
Perspective
Practicing Western Oncology in Shanghai, China: One
Group’s Experience
By David H. Garfield, MD, Harold Brenner, MD, FRCR (Lond), and Lucy Lu, RN
ProMed Cancer Centers–Shanghai, Shanghai, People’s Republic of China
In October 2011, we—David H. Garfield, MD, medical oncologist,
United States; Harold Jacob Brenner, MD, FRCR,
radiation oncologist, Israel; and Lucy Lu, oncology nurse,
Shanghai, China—were part of a group tha... 阅读全帖 |
|
w******y
发帖数: 4871 | 4 Febrile seizures
Author
Marvin A Fishman, MD
Section Editor
Douglas R Nordli, Jr, MD
Deputy Editor
Janet L Wilterdink, MD
Last literature review version 19.1: January 2011 | This topic last updated:
February 3, 2011 (More)
INTRODUCTION — Febrile seizures are a common cause of convulsions in young
children. They occur in 2 to 4 percent of children younger than five years
of age, but the incidence is as high as 15 percent in some populations. This
incidence has been attributed to closer living arr... 阅读全帖 |
|
h*******y
发帖数: 1563 | 5 What: Shares of rare-earth element miner Molycorp (NYSE: MCP ) jumped 10% w
hen a bill to stockpile rare-earth elements was announced in Congress.
So what: Congressman Mike Coffman introduced the bill that would establish a
stockpile of rare-earth elements from U.S. sources -- aka Molycorp and some
day Rare Element Resources (AMEX: REE ) . The U.S. military consumes 5% of
the world’s rare-earth element production. With China already stockpiling t
he elements, the bill views this as a point of ... 阅读全帖 |
|
c*****r
发帖数: 8227 | 6 http://finance.yahoo.com/news/Traders-China-halts-rare-apf-1993092402.html
China has halted exports to Japan of rare earth elements -- which are
crucial for advanced manufacturing -- trading company officials said Friday
amid escalating tensions between the rival Asian powers over a territorial
dispute.
Japan imports 50 percent of China's rare earth shipments. Rare earth are
metallic elements crucial for superconductors, computers, hybrid electric
cars and other high-tech products.
The two tradi |
|
e****3
发帖数: 441 | 7 发信人: Herzog (singularity), 信区: Military
标 题: 影帝又卖稀土了 ：China's Wen says not to curb rare earth exports
发信站: BBS 未名空间站 (Sun Sep 2 15:32:23 2012, 美东)
买办威武！
http://uk.reuters.com/article/2012/08/31/uk-china-rareearths-id
BEIJING | Fri Aug 31, 2012 4:52am BST
(Reuters) - China does not intend to curb exports of rare earths, Premier
Wen Jiabao said on Friday at a forum with visiting German Chancellor Angela
Merkel.
China released a new round of rare earths export quotas this month, raising
its 2012 ... 阅读全帖 |
|
p********1
发帖数: 2785 | 8 这是Amazon上Lin的评论。存在这里做个纪念：
As a Chinese, I lived through that period of time in China. I have similar
family and educational background as hers and suffered during Culture
Revolution as a child. I think her experiences in China mostly, if not all,
are fabricated, imagined, overly exaggerated or deliberately miss leading.
If one just read the media reports about her book, he/she may think that
media might just pick the sensational parts of the book and exaggerated a
little bit to help her to sell ... 阅读全帖 |
|
c**i
发帖数: 6973 | 9 Loretta Chao, Two New Videos, Two Radically Different Chinas. China Real
Time, Jan 24, 2011.
http://blogs.wsj.com/chinarealtime/2011/01/24
/two-new-videos-two-radically-different-images-of-china/
("the other is a short, intensely violent satirical animation about a little
boy who dreams of revolution against a corrupt, abusive government. * * *
In it, a boy dreams about white rabbits, representing regular Chinese
citizens, living under the tyrannical rule of tigers who do nothing as the
rabbits’... 阅读全帖 |
|
l****z
发帖数: 29846 | 10 U.S. drugmaker AbbVie finally wins Dublin's Shire with $55 billion deal
(Reuters) - U.S. drugmaker AbbVie Inc bought Dublin-based Shire Plc on
Friday in a 32 billion pound ($54.7 billion) deal that will allow it to
slash its tax bill by relocating to Britain.
The London-listed company, which makes expensive medicines to treat rare
diseases, fought off four earlier bids from AbbVie until the U.S. firm
raised its price to 52.48 pounds per share - made up of 24.44 pounds in cash
and 0.8960 new AbbV... 阅读全帖 |
|
s***q
发帖数: 10585 | 11 只要是一辆车，都能找到一大堆歧义
ACURA
Always Catching Up, Rarely Ahead
Asia's Curse Upon Rural America
A Car Usually Rarely Appreciates
AMC
Always Made Crap
Autos May Combust
AUDI
Accelerates Under Demonic Influence
Always Unsafe Designs Implemented
Always Under Diagnostic Inspection
Always Upside-down, Double Interest
Another Understated Dealer Incentive
A Used Dodge Incognito
BMW
Bob Marley and the Wailers
Beautiful Mechanical Wonder
Beautiful Models Wanted
Bavarian Manure Wagon
Biggest Metal Waste
Big Money... 阅读全帖 |
|
L***H
发帖数: 40 | 12 Please come take a look and pick up any time. All remain under very good
condition and are sold at a great price. Please message me on MITBBS to
arrange for appointment/pickup. Thank you!
1) Gazelle Edge Exercise Machine, originally $100, 1-yr old, NOW FOR $60
2) Queen size Mattress with Box Spring and bed frame, originally $700, 7-yr
old, NOW FOR $200 (pick up in Feb)
3) Magic Chef Microwave (MCD795SW), 950W, originally $70, 4-yr old but
rarely used, NOW FOR $25
4) Kenmore Window AC (2537005101... 阅读全帖 |
|
S*********y
发帖数: 261 | 13 Moving sale
我在harbor point，你如果买得多，我可以帮助开车送过去。
大同不锈钢电饭锅，去年买的，当时是40块，现在买15块。
1.Almost new big soup pot, original price is about $30. It is rarely used
and now sell for $10.
一个大汤锅，可以煮汤，煮面条饺子等。去年买的，只用过几次。新的时候大约30块，
现在卖15块。买锅送可以捞饺子的漏勺。
2.Flat pan, original price is about $20 and it is rarely used. Now sell for
$10.
平底锅，也是去年买的，只用过几次。新的时候大约20，现在卖10块。
3.Baking pans, a round one and a square one, $2 for both.
烤盘，去年买的，1块钱一个。
4.Baking safe Sur La table brand small dishes (can be used as small sauce
bowls t... 阅读全帖 |
|
L***H
发帖数: 40 | 14 Please come take a look and pick up any time. All remain under very good
condition and are sold at a great price. Please message me on MITBBS to
arrange for appointment/pickup. Thank you!
1) Gazelle Edge Exercise Machine, originally $100, 1-yr old, NOW FOR $60
2) Queen size Mattress with Box Spring and bed frame, originally $700, 7-yr
old, NOW FOR $200 (pick up in Feb)
3) Magic Chef Microwave (MCD795SW), 950W, originally $70, 4-yr old but
rarely used, NOW FOR $25
4) Kenmore Window AC (2537005101... 阅读全帖 |
|
g********r
发帖数: 94 | 15 我也是类风湿，美国的医生给吃HYDROXYCHLOROQUINE (Plaquenil) [hi-drox-ee-CLOR-
oh-kwin]的确见效了，不过后来我停了，因为怀疑有色素沉淀现象。
How to use this medication
What is it
Hydroxychloroquine is a medication used to treat different types of
arthritis. While taking this medicine it is important to have your eyes
checked at least once a year.
Take it every day
This medicine is taken either once or twice a day.
Tablets
It only comes in 200 mg tablets. The usual dose is between one and two
tablets per day (200 to 400 mg per day).
Be p... 阅读全帖 |
|
c*******u
发帖数: 12899 | 16 ☆─────────────────────────────────────☆
aprilxu (妞妞是个乖宝宝) 于 (Fri Mar 30 00:33:44 2012, 美东) 提到:
我已经很久很久不发帖了，自从我女儿确诊以后，我就再没有心思在网上发帖和回帖了
。这几天看了一些关于残疾孩子和流产的帖子，我忍不住想说几句我自己的经历，也算
是一种倾诉吧。
我女儿快三岁了，又漂亮又聪明，可是，她不会走路，而且永远也不会。她患有SMA (
Spinal Muscular Atrophy) 。这是一种genetic disorder，由于基因缺失身体无
法制造一种蛋白，严重影响她的运动神经元和所控制的肌肉，导致肌张力低下。SMA是
一种罕见病，发病率仅为万分之一，由于我和我老公都是携带者（我们都不知道，也没
有家族史），我们的孩子有四分之一的几率患病，我的女儿很不幸。
但是幸运的是，我的女儿不是最严重的情况。Type 1，也就是最严重的情况，孩子一般
不会活过两岁。我女儿属于type 2，医生没说能活多久，只是告诉我们，最终依靠仪器
，她也许能活到成年，能上college，但是... 阅读全帖 |
|
n***p
发帖数: 7668 | 17 有人提到一个英国的调查结果,这里是链接.当然,因为英国跟中国文化有差异,大家自己
看看就算了。
http://www.telegraph.co.uk/women/mother-tongue/8429386/Want-to-
'BEST' TO 'WORST' COMBINATIONS OF CHILDREN:
1.Two girls
2.One boy and one girl
3.Two boys
4.Three girls
5.Three boys
6.Four boys
7.Two girls and one boy
8.Two boys and one girl
9.Three boys and one girl
10.Three girls and one boy
11.Two boys and two girls
12.Four girls
BENEFITS OF HAVING TWO GIRLS:
1.Rarely noisy
2.Help around the house
3.Very few fights and arguments
4.Quite easy to r... 阅读全帖 |
|
s*********5
发帖数: 5637 | 18 想不到这个帖子引发了大家这么热烈的讨论。谢谢那些花了很多时间砌砖的朋友来严肃讨论这件事情。
大家应该都听说过环境对基因会产生诱发或抑制的作用吧。例如说科学家发现有种“害羞基因”，但如果有这个基因的孩子，他们的父母对他如果没有过分保护并带他从小去各种各样的环境，这种基因的影响就会被抑制，孩子还是可以落落大方。
我自己觉得性倾向虽然可能有基因的影响在里面，但以下几个数据让我觉得it is much more by choice rather than by birth.或者说后天的经历和环境的影响远大于先天的影响。
1.同性恋的倾向与文化和宗教有着非常大的影响
Dr. Neil Whitehead is a research scientist and biochemist from New Zealand
and is his wife Briar Whitehead is a writer.[19] Dr. Whitehead coauthored a
book with with his wife entitled My Genes Made Me Do it - a scienti... 阅读全帖 |
|
|
W******e
发帖数: 3319 | 20 1. Introduction
My interest in studying creativity was inspired by the frustrations that I
felt as a student, then as a professor. I wanted to know how I could
encourage creativity in myself, my students, and my colleagues.
Politicians, industrial managers, academic administrators, and other leaders
often say that innovation is critical to the future of civilization, our
country, their company, etc. But in practice, these same people often act as
if innovation is an evil that must be suppressed,... 阅读全帖 |
|
f**********1
发帖数: 18 | 21 联系方式：
微信:185061164
qq: 2757578348
qq群： 559073275
不要站内信。
以下产品都收的，如果一时收满了，过1个月左右还是要的一般。科颜氏可以和倩碧黄
油，fresh，兰蔻粉水，雅诗兰黛anr系列，mac唇膏，试管香水一起出给我。谢谢。
英文名称 采购价格
125ml大高保湿 Ultra Facial Cream 42.00
50ml小高保湿 Ultra Facial Cream 24.20
250ml高保湿水 Ultra Facial Toner 15.00
150ml高保湿洁面 Ultra Facial Cleanser 15.00
125ml高保湿乳液 Ultra Facial Moisturizer 23.38
125ml高保湿面膜 Ultra Facial Overnight Hydrating Masque 29.75
50ml医学保湿导入精华 Hydro-Plumping Re-Texturizing S... 阅读全帖 |
|
s*******e
发帖数: 432 | 22 China's export is about 97% of the rare earth metal world trade.
Most rare earth processor in western countries have stopped producing since its low price can not cover their cost.
Currently China has 37% world reserve, US has 13% world reserve.
China has reduce 70% of export quote in July and rare earth price has sky-rocket, some heavy rare earth has rise 10 times since then. So any ban like this will only raise the price
earth |
|
d****r
发帖数: 1499 | 23 DOW JONES NEWSWIRES
Molycorp Inc. (MCP) acquired a 90% stake in rare-earth processing company
AS Silmet in a deal valued at about $89 million , giving Molycorp a
foothold in Europe and doubling its production capacity.
The rare-earth mining company acquired about 80% of AS Silmet shares from
AS Silmet Group , which will retain a 10% stake, and the remainder from
Treibacher Industrie AG .(R.TBI).
The European facilities, based in Estonia , will process rare-earth
feedstocks from Molycor... 阅读全帖 |
|
b*****s
发帖数: 1475 | 24 Tuesday, May 1, 10:26 AM Rare earth stocks trade higher following a report
that China will begin issuing a new special tax permit to all rare earth
producers that will be required in order to legally produce light rare
earths, such as cerium and lanthanum, and the concentrates from which all
rare earths are derived. MCP +2.7%, AVL +2%, REE +1.3%.
老天爷，俺什么时候才能出水啊 。。。 |
|
b********s
发帖数: 272 | 25 Rare-Earths Miner Molycorp Prepares to File for Bankruptcy Protection
Molycorp Inc., the only U.S. miner and processor of rare-earths elements,
plans to file for chapter 11 bankruptcy protection as soon as this month to
cut its $1.7 billion debt load, according to people familiar with the matter.
The Greenwood Village, Colo.-based company is completing a plan that would
involve senior bondholders exchanging some or all of their debt for
ownership of the company, some of the people said. It is ex... 阅读全帖 |
|
S*********y
发帖数: 261 | 26 Moving sale
我在harbor point，你如果买得多，我可以帮助开车送过去。
大同不锈钢电饭锅，去年买的，当时是40块，现在买15块。
1.Almost new big soup pot, original price is about $30. It is rarely used
and now sell for $10.
一个大汤锅，可以煮汤，煮面条饺子等。去年买的，只用过几次。新的时候大约30块，
现在卖15块。买锅送可以捞饺子的漏勺。
2.Flat pan, original price is about $20 and it is rarely used. Now sell for
$10.
平底锅，也是去年买的，只用过几次。新的时候大约20，现在卖10块。
3.Baking pans, a round one and a square one, $2 for both.
烤盘，去年买的，1块钱一个。
4.Baking safe Sur La table brand small dishes (can be used as small sauce
bowls t... 阅读全帖 |
|
s*********5
发帖数: 5637 | 27 我没有想到我在parenting版的帖子会在一天内产生两百多个回帖，大家对同性恋到底
是由于先天的还是后天的影响产生了热情的讨论。我也只是希望提高大家对这件事的
关注。我们可能不能改变对方的想法，但请你们也尊重我们希望孩子不在小学时就被过
早‘灌输’到关于性倾向的讨论。
----------------
发信人: spectrum805 (spectrum805), 信区: Parenting
标 题: Re: 如果你孩子的老师给他们讲王子娶了王子的幸福生活你会怎样
发信站: BBS 未名空间站 (Thu Sep 29 01:58:11 2011, 美东)
想不到这个帖子引发了大家这么热烈的讨论。谢谢那些花了很多时间砌砖的朋友来严肃
讨论这件事情。
大家应该都听说过环境对基因会产生诱发或抑制的作用吧。例如说科学家发现有种“害
羞基因”，但如果有这个基因的孩子，他们的父母对他如果没有过分保护并带他从小去
各种各样的环境，这种基因的影响就会被抑制，孩子还是可以落落大方。
我自己觉得性倾向虽然可能有基因的影响在里面，但以下几个数据让我觉得it is much
more by choice ... 阅读全帖 |
|
d******1
发帖数: 140 | 28 有一些东西已经被卖了，剩下的在这里
版主手下留情，bow！
Everything needs to be sold before 8/20, will give to best offer
pictures are here:
http://sandiego.craigslist.org/csd/for/5171829075.html
2. Dinning table with 4 chairs and a bench and wood carpet $ 550
Brough from Ashely Oct. 2014, they are in perfect condition
Table only for $400, originally 800
chair $80 each, orginally $225/$186
Bench: $100
Wood carpet: $100
The table can be stretched for 10 person
3. Wash and Dryer $320
Pretty New. Brought from home depot July... 阅读全帖 |
|
|
H****w
发帖数: 1632 | 30 Billy Walter and the computer group:
The Arrests
He was in the bed sleeping when the two men walked into his bedroom.
Billy
Walters sleeps in a big clean bed in Las Vegas, in a small but elaborate
home renovated to his liking, with palm trees and white flowerpots and
two
satellite dishes in the yard, and four large televisions in the den, and
a
security guard who sits just out of sight behind the shrubs across the
street. This environment was disrupted early last Jan. 5, when the two
strangers i... 阅读全帖 |
|
C******s
发帖数: 1917 | 31 （三）白日畅滑须纵酒
民以食为天，食以酒为先。雪民亦不能免俗。大家远道而来，虽然主要是为了惠斯勒的
雪，但是滑雪之外的事情也不能含糊。
大军未到，粮草先行。滑雪聚会的主要组织者，把组织过程演绎得真正淋漓尽致。不仅
把惠斯勒所有的好餐馆，事先作了仔细的调查研究，而且临行前数天就预订了每一天的
晚餐。人家比我们更远道而来，却提前两天到达温哥华，并找到当地口碑最好的华人食
品店购买可口的点心和食品。更令我佩服得五体投地的是，一见面就拿出几十页的做成
企划书的行程计划，每一天都有一个标签，里面包含当天的滑雪教练安排，餐馆预约，
餐馆地址及地图，还有所有其它活动，应有尽有，不尽其详。
惠斯勒的餐馆总体来说属上乘，虽然算不上物美价廉。因为正赶上圣诞节，每个好餐馆
几乎都是暴满，行前预定真是明智之举。
印象最深的几个晚餐，首先是在惠斯勒村内的意大利餐馆Il Caminetto di Umberto。
那一餐我们六个大人大多点的都是海鲜，几种不同的鱼做得都不错，是意餐中的上品。
佐餐开了两瓶红酒，第一瓶是2007年的阿玛罗尼（Tommasi Amarone），用意大利酒配
意大利菜。不过阿玛罗尼口感较... 阅读全帖 |
|
l*******o
发帖数: 12469 | 32 煤黑，煤灰，煤粉和煤轮的机会
Interview: Lionel Messi on His Sport, Cristiano Ronaldo — and Argentina
By Bobby Ghosh / Barcelona
Read more: http://www.time.com/time/world/article/0,8599,2105369,00.html#ixzz1kev3H83Y
Journalists are evenly split on whether or not they should interview their
personal heroes: some say you shouldn't because you'll find your idol has
feet of clay, others argue the opportunity is too good to miss. For me, it
was a no-brainer. Lionel Messi is not only the shining star of my favorite
... 阅读全帖 |
|
p*********l
发帖数: 26270 | 33 说明你本来就不是爱牛排的人，卡卡，well done。。。我一向吃medium rare，现在发
现很多牛排馆都越烤越老了，所以开始吃rare了。。。Florentine steak很对我胃口，
是真正的medium rare，在NYC好多店的rare才能这么嫩 :D
你这图上明显太老了，哈～ 看我那盘子里都是血水～
我吃得爽死了，1.1 kg几乎都是我吃的，回到酒店才发现不能躺下来睡觉，肚子太重了
，压迫心肺，于是爬起来做了一套yoga来消食。。。 |
|
p*********l
发帖数: 26270 | 34 说明你本来就不是爱牛排的人，卡卡，well done。。。我一向吃medium rare，现在发
现很多牛排馆都越烤越老了，所以开始吃rare了。。。Florentine steak很对我胃口，
是真正的medium rare，在NYC好多店的rare才能这么嫩 :D
你这图上明显太老了，哈～ 看我那盘子里都是血水～
我吃得爽死了，1.1 kg几乎都是我吃的，回到酒店才发现不能躺下来睡觉，肚子太重了
，压迫心肺，于是爬起来做了一套yoga来消食。。。 |
|
t******g
发帖数: 17520 | 35 M Guaranteed Drop
E Common Drop
D Semi Common Drop
C Semi Rare Drop
B Quite Rare Drop
A Rare Drop
S Very Rare Drop
item drop 分等级
10个人性就到顶了， 210点， 还要+的话就要ring 或者另外一个啥了， 可以加到410
， patch1.05 以后 drop rate 涨了不少
就是不知道point 对drop rate 是个怎样的公式 |
|
b*s
发帖数: 82482 | 36 刚才在路上，说不明白。除了那本书以外，原来在纽约客上Galdwell的文章可以给出详
细一些的例子吧：
http://www.gladwell.com/2008/2008_10_20_a_latebloomers.html
Articles from the New Yorker
Late Bloomers
October 20, 2008Annals of Culture
Why do we equate genius with precocity?
1.
Ben Fountain was an associate in the real-estate practice at the Dallas
offices of Akin, Gump, Strauss, Hauer & Feld, just a few years out of law
school, when he decided he wanted to write fiction. The only thing Fountain
had ever published was a law-review article. H... 阅读全帖 |
|
g********y
发帖数: 236 | 37 here is another good case on how to identify those lower life form (LLF).
http://www.mitbbs.com/article_t/WaterWorld/1719001.html
First, let me lay out ground rules. LLF do not have any principle. They are
just worm like animals. The do not have the concepts universal to real
human being, such as love, dignity. They will trade anything to advance
themselves.
This principle apply to both of them.
To start with, 衣 is such a roach, what does moron like that even know about
Marxism. People like tha... 阅读全帖 |
|
H****N
发帖数: 997 | 38 Just to counter you argument on eye evolution, Pax6 is required for eye
formation in all animals examined so far, strongly arguing for a single
origin of all eyes during evolution. Of course, further evolution has
happened along different phylogenetic lineages, but no eye has got away from
the basic structure of photoreceptor-pigment cell.
As to your "巧合", you are making many assumptions again. If evolution is
directional as you think, covengent evlution events should not be as rare as
they are.... 阅读全帖 |
|
l**********n
发帖数: 201 | 39 已经有discussion了，至少在某些领域。去Science上留comments吧：
http://www.sciencemag.org/content/335/6076/1558.full
Science 30 March 2012:
Vol. 335 no. 6076 pp. 1558-1561
DOI: 10.1126/science.335.6076.1558
News Focus
Psychology Research
Psychology's Bold Initiative
Siri Carpenter*
In an unusual attempt at scientific self-examination, psychology researchers
are scrutinizing the reproducibility of work in their field.
Pick up the January 2008 issue of Psychological Science, turn to page 49,
and you'll find a... 阅读全帖 |
|
i*e
发帖数: 352 | 40 没有啥能100%万无一失的，BatchPD那个估计common SNPs都已经考虑了，rare的不清楚
，不过rare的话碰到的概率也小很多。此外individual个体还可能有未知的rare
variants，这个就更控制不了了。
看你之前所说，感觉是用来做validation不是detection的，所以万一有那么几个
readout不一致，换对引物或者用其他方法再验证一下。
我有可能记错，不错homopolymer和repeats在exons没有在3‘或者5’端频繁，有碰到
再分别单个手工设计引物好了。
我自己那个script，主要针对自己的实验目的，是WGS的variant validation，主要是
SNVs和小的Indels，也不单单是exonic，所以大多数面向exons的predesigned primers
就无用了，再说SNPs也没有被先排除。
设计引物的时候，input是VCF，已知的common和rare SNPs （dbSNP142）都有先
flagged，然后primer3的结果只取第一个output，之后in silico PCR再过一次，没过
的重新滚... 阅读全帖 |
|
