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全部话题 - 话题: receptors
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S*****s
发帖数: 287
1
Inverse agonist?
http://en.wikipedia.org/wiki/Inverse_agonist
In pharmacology, an inverse agonist is an agent that binds to the same
receptor binding-site as an agonist for that receptor and reverses
constitutive activity of receptors.[1] Inverse agonists exert the opposite
pharmacological effect of a receptor agonist. Inverse agonists are effective
against certain types of receptors (e.g., certain histamine receptors and
GABA receptors[2]) that have intrinsic activity without the action of a
l... 阅读全帖
s******9
发帖数: 283
2
NCBI protein database一般都有标注吧。
FEATURES Location/Qualifiers
source 1..503
/organism="Homo sapiens"
/db_xref="taxon:9606"
gene 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
Protein 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
... 阅读全帖
s**u
发帖数: 9035
3
Neurological Diseases and Receptor Pharmacology--Post-doctoral position at
the NIA

Department of Health and Human Services
National Institutes of Health
National Institute on Aging


The National Institute on Aging (NIA), a major research component of
the National Institutes of Health (NIH) and the Department of Health
and Human Services (DHHS), is recruiting for a postdoctoral research
position in the Receptor Pharmacology Unit of the Laboratory of
Neur... 阅读全帖
s**u
发帖数: 9035
4
Neurological Diseases and Receptor Pharmacology--Post-doctoral position at the NIA

Department of Health and Human Services
National Institutes of Health
National Institute on Aging


The National Institute on Aging (NIA), a major research component of
the National Institutes of Health (NIH) and the Department of Health
and Human Services (DHHS), is recruiting for a postdoctoral research
position in the Receptor Pharmacology Unit of the Laboratory of
N... 阅读全帖
b*****l
发帖数: 9499
5
呃。。。有些糊涂。。。 agonist/antagonist 是针对模拟一种 ligand 的 analog/dr
ug 呢,还是指针对一个 signaling event,还是针对一种 bio-function?
我过去一直理解为,能跟某种 ligand 的 receptor binding 的东西,如果跟这种 li
gand 引起的作用相同,并且作用的程度类似或者更高的,叫 agonist;如果 binding
但不引起后续的 signaling events 的,也就是说,block 了 receptor 的,甚至引起
相反的作用的,叫做 antagonist;能引起类似的效果,但是不如 ligand 有效的,叫做
semi-agonist,又因为这种 semi-agonist drug 和 ligand 竞争在 receptor 上的 b
inding site,如果 ligand 过量而 receptor 不足时,或者这种 drug 的 affinity 高
到严重阻碍 ligand/receptor binding 时,这种竞争就会导致 signal 减弱,所以这时
这种... 阅读全帖
n**m
发帖数: 7872
6
【 以下文字转载自 loseweight 讨论区 】
发信人: nkmm (nk), 信区: loseweight
标 题: 美国2009肥胖病协会年会摘要1-taste receptor
发信站: BBS 未名空间站 (Thu Oct 29 19:12:17 2009, 美东)
才开会回来,很都有意思的东西,不过没时间,就只写一个先,等过半个月我忙完了继
续往下写 :)
做这个报告的是Robert Margolskee, MD, PhD
我这里简化、普及化一点。
taste receptor,就是辨别各种味道(比如甜味,苦味,酸味等等。这里就只讲甜味了
)的蛋白质。舌头上有taste
receptor(地球人都知道),但是不光舌头上有,肠子里也有!肠子里面的L-cell,一
种分泌荷尔蒙的细胞上面发现了
taste receptor,如果有甜味物质刺激的时候,会分泌GLP-1 (glucagon like peptide
-1),这个荷尔蒙会刺激胰岛素分
泌。进一步的研究发现,不光肠子里有,胰岛里也有,有甜味物质刺激时,会分泌胰岛
素(促进糖,脂肪酸uptake,当然
还有其他作用
p*****m
发帖数: 7030
7
1 你google一下从EPO receptor到serotonin receptor到TRP channel怎么搞出来的
就行了 简单来说就是搞一个没有receptor的cell line,把有receptor表达的cDNA
library转进去 然后加ligand,看下游的phenotype就行了 可以是binding activ
ity,也可以是downstream signaling,等等
2 没有 但是如果你有个范围可以大概用agonist/antigonist猜猜看

specific
t*********1
发帖数: 418
8
假设我所研究的receptor是抑制某一生物活性。其ligand 与receptor结合后,一定是
加强receptor对某生物活性的抑制作用吗?还是也有可能减弱receptor对某生物活性的
抑制作用?
我的初步观察似乎是后者,这样的结果可能是合理的,还是我可能有什么地方出错了呢?
请高手赐教,谢谢。
u*********1
发帖数: 2518
9
那么庞大的Olfactory receptor只和嗅觉有关么?
因为Olfactory receptor也算G protein-coupled receptors这个大类的,是不是也可
能和比如听觉或者别的sensory有关系?
另外,olfactory receptor所有的gene function都了解的清楚么,目前?
谢谢~
b***n
发帖数: 36
10
请问版上有玩GPCR尤其是dopamine receptor的兄弟姐妹不
我现在在做DR internalization, 试了好几种办法,
不管是Membrane biotinylation,
或者是用Flag M2在没有fix之前去label外膜上的receptor
或者是用Radio ligand来做,
都不是很成功,最近试了一下用Sucrose gradient (35%)来分heavy membrane 和light
vesicle, 应该是有看到了一些internalization.
不过最大的问题是用FLAG M2做完WB后看到的并不是一条单一的带,而是一片
的smear,
应该是某些地方没做好,所以D2R都聚集了
用的是Biorad的SDS loading dye 配方直接溶解膜
或者也试过RIPA和TBS+10%甘油+1%triton
效果都不是太好
但是看了不少paper貌似人家都是做得出来了
这个事情搞了大半年,一直没能搞定,老板又特别楞,从来不给些建设性的意见,每隔
一段时间都要威胁一下做不出来就开除,已经被威胁了十数次,真是有点窘境穷途了,,
,,还能不能接着... 阅读全帖
m*****0
发帖数: 72
11
来自主题: Pharmaceutical版 - Receptor occupancy 有前途吗
目前在做类似于PD-1的前期工作,通过内部refer申请 receptor occupancy这个工作。
这个receptor occupancy是在 clinical pharm area. 个人觉得偏临床的receptor
occupancy工作是不是好些阿?
问了几个身边朋友,有人觉得receptor occupancy很乏味,每天做routine的工作,PD-
1类型的工作应该更有挑战性,但是我担心工作的稳定性。
thanks a lot
n**m
发帖数: 7872
12
才开会回来,很都有意思的东西,不过没时间,就只写一个先,等过半个月我忙完了继
续往下写 :)
做这个报告的是Robert Margolskee, MD, PhD
我这里简化、普及化一点。
taste receptor,就是辨别各种味道(比如甜味,苦味,酸味等等。这里就只讲甜味了
)的蛋白质。舌头上有taste
receptor(地球人都知道),但是不光舌头上有,肠子里也有!肠子里面的L-cell,一
种分泌荷尔蒙的细胞上面发现了
taste receptor,如果有甜味物质刺激的时候,会分泌GLP-1 (glucagon like peptide
-1),这个荷尔蒙会刺激胰岛素分
泌。进一步的研究发现,不光肠子里有,胰岛里也有,有甜味物质刺激时,会分泌胰岛
素(促进糖,脂肪酸uptake,当然
还有其他作用)。
这个研究的indication是:甜味剂虽然不含卡路里,但还是有可能促进胰岛素分泌,从
而进一步增加fat storage的。而过
量胰岛素分泌可能导致身体对胰岛素的敏感性降低(insulin resistant)以及影响胰岛
的功能,有可能导致糖尿病。
l**********1
发帖数: 5204
13
来自主题: Biology版 - 求助 ligand receptor binding
RE LD
你这问题问的是高中生高考水平或生化系大学3年前水平的话
答案是 stable同位素标定Steroid Legend 然后 assay 然后单离steroid receptor 质谱分析receptor里边有无
那同位素
如果问的是PD PhD thesis 水平
输入你那个目标steroid receptor 之
Protein ID Protein Sequence
然后
http://www.pathblast.org/
查一下先
偶这也是借以色列的某教授的花
http://www.cs.tau.ac.il/~roded/
Selected Web-Servers / Software:
· ANAT
· Torque
· NetworkBLAST
· EXPANDER
献佛仅此而已
C***Y
发帖数: 142
14
来自主题: Faculty版 - 求 sigma-1 receptor plasmid
如果哪位大侠有sigma-1 receptor plasmid, 敬请站内PM我。最近需要overexpress
sigma-1 receptor. 万分感谢!!!
C***Y
发帖数: 142
15
来自主题: Postdoc版 - 求 sigma-1 receptor plasmid
如果哪位大侠有sigma-1 receptor plasmid, 敬请站内PM我。最近需要overexpress
sigma-1 receptor. 万分感谢!!!
h*****o
发帖数: 342
16
来自主题: Biology版 - In vivo receptor blocking with antibody
there is no set rules for that... you probably need to try a few times.
1. iv is more for acute reponse. ip is slow but maybe last longer. it
depends on what receptor you are looking at and what tissue are they located
2. somebody probably has done that, or the data sheet of your mAb suggest a
starting dose to try. you might need to do a escalating dose to find out the
optimal dose
3. again it depends on your specific receptor, their turnover rate, the
halflife of your mAb, etc...
4. often peopl
D******9
发帖数: 2665
17
I would like to study the cell signaling pathway initiated by a cell surface
ligand. However, I do not know its receptor. What is the best way to find
its receptor? Any good ideas? Thanks a lot.
p****l
发帖数: 291
18
yeah, it is really difficult. One of my projects is quite similar.
I finished the step crosslink and IP, and sent the sample for MASS.
But to confirm the receptor is the protein reported by MASS, still need a
lot of time.
I prefer the method you recommended, transfer cDNA library to a cell line
which does not express the receptor. Screen the positive clone.
b******d
发帖数: 149
19
来自主题: Biology版 - GPR120 Is an Omega-3 Fatty Acid Receptor
Interesting study. Whenever the targets/mechanisms of a natural product
is discovered, it's a top paper...
http://www.cell.com/fulltext/S0092-8674(10)00888-3?switch=standard
Omega-3 fatty acids (ω-3 FAs), DHA and EPA, exert anti-inflammatory
effects, but the mechanisms are poorly understood. Here, we show that the
G protein-coupled receptor 120 (GPR120) functions as an ω-3 FA
receptor/sensor. Stimulation of GPR120 with ω-3 FAs or a chemical agonist
causes broad anti-inflammatory effects in mono
D*a
发帖数: 6830
20
1
If you do this experiment by injecting this ligand continuously, the
receptor could become resistant afterwards.
2
another example (n.b. NOT necessarily relevant to point 1)
growth hormone (GH) binds to its receptor and triggers a series of
downstream events, eg Stat5, cyp45 or something (cannt remember)
BUT males secrete GH in big and regular peaks, while females secrete smaller
peaks and irregularly (in another word, females secrete GH contiuously,
according to some big cow, 我持保留意见)
So this ... 阅读全帖
S*****s
发帖数: 287
21
同意。理论上来讲 antagonist 的作用是结合到 receptor 的 binding pocket 上让
agonist 不能结合,本身并不能抑制 receptor 的活性。
h****k
发帖数: 182
22
谢ls,如果蛋白本身是单体,使用crosslinking后会不会造成假象,检测结果为dimer
或者multimer??
另外co-IP的时候使用该receptor的抗体,如果是单体,该抗体会不会聚集这些
receptor,而成为dimer或者multimer??
v***a
发帖数: 1242
23
一个non-receptor tyrosine kinase,在我进行某种与cell migration有关的诱导后,
它的protein level下降了,但它其它的磷酸化形式都没有变化(WB结果)。根据其它
的条件,实验中的cell migration应该增加,但我查了很多文献,几乎所有的
reference都说这个non-receptor tyrosine kinase的protein level下降的话是因为
cell migration减少。
难道是我的观察有误吗?但实验重复来重复去,结果都高度一致,真是想不通啊!上来
问问大家,看看有没有遇到类似情况的?
p*****m
发帖数: 7030
24
来自主题: Biology版 - How to identify a receptor?
你这是考试题么。。lol
是考试题的话:
expression cloning (e.g. EPO receptor)
affinity purification (e.g. ACh receptor)
bioinfo (只适用于已知其他物种里的ortholog 太多了这个)
如果是project:
问问老板为什么要让你做这个。。抗议之
l*****4
发帖数: 267
25
来自主题: Biology版 - 求助 ligand receptor binding
非常感谢你的回复
有几个问题再问一下
也许我没说清楚我也要的是在动物体内直接实验证明两者的相互作用
第二的生物信息学的方法好像不适用
第一个方法
如果你的结果是negative的
你如何证明 同位素标记ligand和receptor的分离不是因为提蛋白或assay或其他什么过
程中两者分离了呢

质谱分析receptor里边有无
C***Y
发帖数: 142
26
来自主题: Biology版 - 求 sigma-1 receptor plasmid
如果哪位大侠有sigma-1 receptor plasmid, 敬请站内PM我。最近需要overexpress
sigma-1 receptor. 万分感谢!!!
a******1
发帖数: 10
27
买抗体,搜索R&D网站interferon Alpha/Beta Receptor Chain 2没有流式抗体,搜索
CD118有流式抗体,显示是LIFR a链,但有些网站上又写Interferon Alpha/Beta
Receptor Chain 2 (CD118) Human, Antibody。咋回事,彻底糊涂了。
t*****i
发帖数: 13
28
来自主题: Biology版 - DNA-DNA vs Ligand-receptor binding
问个基础的生化问题:
ssDNA之间的结合力(假设是100bp)和Ligand-receptor的结合力比起来哪个更强?哪
里可以查到常见的 Ligand-receptor组合的 Kd或者IC50?
谢谢!
l*******6
发帖数: 34
29
FA2010 page 232 states beta-2 receptor increases insulin release. But FA2009
page 226 says beta-2 receptor increases glucagon release. Which one is
correct?
It seems 2010 FA is correct. But UW has an explanation about the target
organ of beta-2 adrenergic blockade is glucagon secretion. Kind of confused.
Please kindly clarify. Thank you.
l*******6
发帖数: 34
30
Thanks Pizza for the "Glucogan刺激Insulin". Therefore, both FA2010 (beta-2 receptor increases insulin release) and FA2009 (beta-2 receptor increases
glucagon release) should be correct.
s****t
发帖数: 31579
31
来自主题: FleaMarket版 - [+] receptor
对方ID:
receptor
Feedback (+/-/0):
+
具体交易内容:
fia points
我的评价:
挺好挺快的,
交易原始贴链接:
http://www.mitbbs.com/article_t/FleaMarket/33441537.html
s**********e
发帖数: 6343
32
来自主题: FleaMarket版 - [+] receptor
对方ID:
receptor
Feedback (+/-/0):
+
具体交易内容:
TYP
我的评价:
很赞的卖家!转点迅速,交易顺利!
交易原始贴链接:
http://www.mitbbs.com/article_t0/FleaMarket/34365505.html
m**p
发帖数: 202
33
【 以下文字转载自 Biology 讨论区 】
发信人: memp (memp), 信区: Biology
标 题: 求助:怎么知道这个cell line有没有glumate receptor
发信站: BBS 未名空间站 (Mon Nov 9 22:39:14 2009, 美东)
也就是加glutumate的时候会不会有Ca2+ influx
想找一个tumor的cell line(因为好养),但是要符合以上标准
这个cell line from ATCC 如何?
Organism: Mus musculus (mouse)
Morphology:
Source: Organ: brain
Cell Type: neuron;
Cellular Products: dopamine; norepinephrine; synaptophysin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or
regulatory permits may be required for the
a****c
发帖数: 339
34
杂志是Biochemistry Insights发的增刊Receptor-ligand interactions and drug
design
。Pubmed indexed。现无impact factor。
任务:联系5篇文章,体裁不限。最后几个editor合写一片editorial.
方向:
1) Biochemistry of ligand binding
2) Experimental and computational drug design.
相近的方向也可以。
杂志说可以出推荐信。估计这种小杂志也就是靠申绿卡的人来拉文章挣钱了。
请站内联系。我会把杂志的instruction发给您。谢谢。
c****i
发帖数: 40
35
Impact factor: 3.138
需要有研究过lipoprotein scavenger receptor-binding properties以及antigen-
antibody binding的reviewer
站内邮件联系,麻烦给我你的名字,email邮箱, research background, instrument
expertise。我会foward给editor的。
h*******n
发帖数: 2052
36
来自主题: Biology版 - In vivo receptor blocking with antibody
I plan to perform in vivo immuno-receptor blocking with antibody and examine the effect of host-defense.
Where can I find the information below:
1. Ways of injection (i.v., i.p., etc.)
2. Dose of each injection
3. How often do I inject the mAb?
4. For how long do I inject the mAb?
I cannot find any paper describing similar experiment with my specific mAb.
Any suggestion is appreciated.
c*****i
发帖数: 1392
37
来自主题: Biology版 - In vivo receptor blocking with antibody
all your questions are dependent on which receptor you are trying to
block....

examine the effect of host-defense.
mAb.
c**n
发帖数: 73
38
来自主题: Biology版 - In vivo receptor blocking with antibody

examine the effect of host-defense.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~```
depends on specific cell types your receptor is expressed on
you need much less if you are targeting circulating cell by i.v
depends on the first question.
if i.v., I would say 60-80 ug would be a good dose to start with.
It also depends on the antibody though.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
depends on the nature of your experiment
Chronic or acute
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~`
check anything that
p*****m
发帖数: 7030
39
expression cloning, the classic method
or RNAi screen, or educated guess, or to test known receptor agonist/
antigonist library

surface
D******9
发帖数: 2665
40
many thanks for your answer. Can you give a little bit details about
expression cloning? Is the receptor agonist/antigonist library very specific
? Thx
g*****n
发帖数: 241
41
来自主题: Biology版 - 请教:insulin-receptor binding assay
我想做一个insulin-receptor binding assay,请问有没有什么protocol啊?我找了好
久都没找到
非常感谢!
A******y
发帖数: 2041
42
The problem with some receptors is that depending on the situation and
ligand, it could be either antagonist or agonist. Is the ligand known
agonist? Is there known agnoist and you can use? A lot of immuno-system
function on a timing system to determine the final signal, but kinetic these
days is a lost art.
h****k
发帖数: 182
43
请问有什么好的方法或者实验可以检测membrane bound receptor 是monomer,dimer,
还是multimer?
s***o
发帖数: 1189
44
来自主题: Biology版 - How to identify a receptor?
It is written in textbook.
but here is some hint, the identified receptors are mostly named according
to their noticeable ligands.
M*****n
发帖数: 16729
45
来自主题: Biology版 - How to identify a receptor?
这个题目俺当年也做过。
也可以用genetics.
不过要运气好,才能找到receptor。
g*********3
发帖数: 177
46
来自主题: Biology版 - amino acid/ receptor与mTOR
1. Do we know any other signal pathways mediating amino acid response?
发现大部分关于amino acid的研究是关于mTOR的和mTOR相关的。
2. 上次版上有人提到了amino acid receptor是个悬而未决的问题,不知道是哪位牛提
到的。能展开说说吗?自己查了一下,确实研究的不是很多。
c*********7
发帖数: 148
47
来自主题: Biology版 - amino acid/ receptor与mTOR
一看到receptor,我自动就想到结晶,然后大家都懂得。。。
l*****4
发帖数: 267
48
来自主题: Biology版 - 求助 ligand receptor binding
通过什么实验可以在动物体内直接证明 steroid 和不和 steroid receptor 结合?
谢谢
h****k
发帖数: 182
49
来自主题: Biology版 - ligand receptor interaction
用IP能检测得到,但是把receptor表达在cell line上用flow cytometry却检测不到,
有人遇到过这种情况么?
b*******y
发帖数: 439
50
怎么证明一个膜蛋白是 receptor?
1 2 3 4 5 6 7 8 9 10 下页 末页 (共10页)