j*p
发帖数: 411 | 1 本人在wet lab里面做纯数据分析，for NGS data analysis, 简单介绍一些自己接触过
，并且觉得挺有用的工具，说的有点杂，权作抛砖引玉，还请不吝赐教。
Next-Gen sequencing(NGS)和现在正在发展的3rd-gen sequencing将会在生物学研究中
被越来越广泛应用。不管你信不信，反正我信了。一是基于实验成本的降低（$1k
whole-genome sequencing is coming），越来越多的实验室可以操作；二是可以提供
相对low throughput experiment多的多的数据和信息，可以看到很多从前看不到的东
西；三是sequencer本身对测序的准确性正在逐渐提高，所以实验固有错误率降低；四
是各种算法的成熟应用，这使得很多由于实验产生的误差在出数据后通过对数据的分析
得以过滤。按照library preparation来分，NGS主要有DNA-seq和RNA-seq
DNA-seq is usually used as ChIP-seq to study transcription factor(TF)-DNA
bi... 阅读全帖 |
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G****e
发帖数: 1912 | 2 https://www.theepochtimes.com/scientific-puzzles-surrounding-the-wuhan-novel
-coronavirus_3225405.html
Scientific Puzzles Surrounding the Wuhan Novel Coronavirus
BY YUHONG DONG February 3, 2020 Updated: February 6, 2020FONT BFONT SText
size Print
Analysis
The sudden outbreak of the Wuhan Novel Coronavirus (2019-nCoV) has resulted
in all of China’s Hubei Province and three major cities in Zhejiang
Province being subjected to quarantine. Other nations are anxiously trying
to get their people out o... 阅读全帖 |
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l***d
发帖数: 1828 | 3 any comments?
http://www.nature.com/news/2011/110519/full/news.2011.304.html#
All science students learn the 'central dogma' of molecular biology: that
the sequence of bases encoded in DNA determines the sequence of amino acids
that makes up the corresponding proteins. But now researchers suggest that
human cells may complicate this tidy picture by making many proteins that do
not match their underlying DNA sequences.
In work published today in Science1, Vivian Cheung at the University of
Pennsy... 阅读全帖 |
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W*********n
发帖数: 775 | 4 sorry for my misunderstanding.
Yes, you are right, I need to find the Translation Start Site or region
around CDS starting point.
Now, I have found the Tranlation Start Site, and also I can make sure the
downstream coding sequences after the TSS. So now I need a comfirmed 5'UTR
sequence, at least some of the sequence near the TSS. Do you think the
unstream of the TSS in the gemome is 5'UTR ? or at least we can make sure of
a samll seuquence before the TSS?
I seed the Coding sequence and 300bp up... 阅读全帖 |
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D**o
发帖数: 2653 | 5 注意作者 \author{YHBKJ}
Atiyah-Bott Localization 1
2012-09-05 09:24:19
\documentclass[a4paper,12pt]{article}
\usepackage{amsfonts}
\usepackage{amsmath,amsthm,amssymb}
\usepackage{CJK,graphicx}
\usepackage{amscd}
\usepackage{amssymb}
\newtheorem{theorem}{Theorem}[section]
\newtheorem{corollary}{Corollary}[section]
\newtheorem{definition}{Definition}[section]
\newtheorem{lemma}{Lemma}[section]
\begin{document}
\title{\textbf{\Huge{Atiyah-Bott Localization 1}}}\author{YHBKJ}\date{}\
maketitle
\begin{ab... 阅读全帖 |
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s*****n
发帖数: 162 | 6 Suppose you are developing a search engineer. Given a sequence of
numbers as a query string. You need to design algorithm/data structure such
that it can return whether this number sequence belongs to some famous
number sequences. For example, if the query string is “0 1 1 2 3 5”, it
might belong to a Fibonacci sequence. Note that the given sequence may not
always start from the beginning of a known number sequence.
My idea:
For those "famous" number sequences, we may cut them into smaller seque... 阅读全帖 |
|
b****n
发帖数: 8 | 7 Address:
Email:
Cell phone:
Work phone:
Oct 28, 2013
Department of Homeland Security
USCIS Nebraska Service Center
P.O. Box 87103
Lincoln, NE 68501-7103
I-140 Immigration Visa Petition Under First Preference EB-1
Petitioner/Beneficiary: XX Z., Ph.D.
Category of Petition: Alien of Extraordinary Ability
Classification Sought: 203(b)(1)(A)
Nature of Submission: Original Submission
Dear Immigration Officer,
This letter is respectfully submitted in s... 阅读全帖 |
|
b****n
发帖数: 8 | 8 Address:
Email:
Cell phone:
Work phone:
Oct 28, 2013
Department of Homeland Security
USCIS Nebraska Service Center
P.O. Box 87103
Lincoln, NE 68501-7103
I-140 Immigration Visa Petition Under First Preference EB-1
Petitioner/Beneficiary: XX Z., Ph.D.
Category of Petition: Alien of Extraordinary Ability
Classification Sought: 203(b)(1)(A)
Nature of Submission: Original Submission
Dear Immigration Officer,
This letter is respectfully submitted in s... 阅读全帖 |
|
v***a
发帖数: 1242 | 9 要overexpress一个蛋白（full length），在NCBI查它的nucleotide sequence来设计
cloning primer，发现有两个看起来都可信的reference sequence，blast了一下，发
现这两个sequence在CDS区有一部分是相同并连贯的，其它就都不同了。其中sequence
1只是cds，并且是partial cds，没有stop codon；sequence 2是complete cds，包括
非CDS区，好像是由NIH MGC (Mammalian Gene Collection) Project来的，但由
sequence 1衍生出的AA序列却比sequence 2衍生的AA序列还要多50多个AA。
我选择了全长的sequence 2设计primer，一共设计了3对，PCR后都未能得到预期bp的
band（虽然有的PCR出来是single band），我每对primer都挑了最接近预期长度的PCR
product去sequence，但都不是我想要的gene。。。难道是sequence 2本身就有问题么
？（我是挑的非CDS区 |
|
p*******r
发帖数: 59 | 10 Career Opportunities at JCVI Infectious Disease (8 Positions)
The PIs did not ask me to post here, I did this just in case someone is
looking for similar positions but did not see these, and I would be happy to
see some chinese fellows to come. All the PIs are very nice.
All listed at
https://careers.jcvi.org/careers/Careers.aspx
http://www.jcvi.org/
No. 1 & No. 2 Post-Doctoral Fellows
Job Responsibilities
JCVI is looking for two Post-Doctoral Fellows to join the Infectious Disease
Group in our ... 阅读全帖 |
|
o********r
发帖数: 775 | 11 今天的nature news:
http://www.nature.com/news/rna-studies-under-fire-1.10502?gobac
RNA studies under fire
High-profile results challenged over statistical analysis of sequence data.
Erika Check Hayden
25 April 2012
Article tools
Print
Email
Download PDF
Rights and Permissions
Share/bookmark
High-throughput RNA sequencing has yielded some unexpected results in the
past few years — including some that seem to rewrite conventional wisdom in
genetics. But a few of those findings... 阅读全帖 |
|
m***T
发帖数: 11058 | 12 sequencing市场更热闹了。
http://www.genomeweb.com//node/1396761?utm_source=SilverpopMail
Roche Acquires Nanopore Sequencing Firm Genia Technologies for up to $350M
NEW YORK (GenomeWeb) – Roche said today that it will acquire Genia
Technologies for $125 million in cash and up to $225 million in additional
payments tied to milestones. Once the deal closes, Genia will be integrated
into the Roche Sequencing Unit.
Genia, based in Mountain View, Calif., has been developing a single-molecule
sequencing-by-sy... 阅读全帖 |
|
t******l
发帖数: 10908 | 13 接上贴的 "smallest complete set with no redundancy" 的装逼说法：
首先观察可得，对于所有相同长度 sequence (前面的 pair)，只要保存 last number
最小的那个就行了，因为其他的都已经是 dynamic programming 的 decision tree 里
面的 suboptimal subtree，整个 subtree 可以一刀直接 prune 掉。
同理再次观察可得，对于所有相同 last number 的 sequence，只要保存长度最长的那
个就行了。。。这个及以上那条可以得出 ordered 属性，也就是对于保存下来（没有
被这两条规则 prune 掉的）的 sequence，sequence 长度越长，last number 必然越
大。
而新的 number 进来的时候，只能延长 last number 比其小的 sequence 而形成新
sequence，而新的 sequence 的长度也就是增加 1 。。。这个结合上面那个条件，这
样只要找 last number 比其小的最长的 sequen... 阅读全帖 |
|
m***T
发帖数: 11058 | 14 http://www.genomeweb.com//node/1217241?hq_e=el&hq_m=1567445&hq_
NEW YORK (GenomeWeb News) – Thermo Fisher Scientific is acquiring Life
Technologies in a $13.6 billion deal, the firms jointly announced today.
Thermo Fisher was rumored over the past couple of weeks to be in advanced
talks to acquire Life Tech, while private equity firms also had been
reported as working on bids to get a deal done. The transaction signing
comes three months after Life Tech confirmed it had hired Deutsche Bank and
M... 阅读全帖 |
|
发帖数: 1 | 15 Moderately Strong Confirmation of a Laboratory Origin of 2019-nCoV
jameslyonsweiler in Cures February 2, 2020 585 Words
Moderately Strong Confirmation of a Laboratory Origin of 2019-nCoV
On the Origins of the 2019-nCoV Virus, Wuhan, China
James Lyons-Weiler, PhD
Dr. Marc Wathelet commented that he was puzzled about my report of a spike
protein gene homologous to part of the pShuttle-SN vector, given that spike
glycoproteins are found in bat coronavirus. He urged me to analyze the
homology (seque... 阅读全帖 |
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a**c
发帖数: 52 | 16 来自主题: JobHunting版 - G家电面题 Q. Find a vowel product sum of an ASCII string.
> There are are 26 English upper and lower-case letters. Consider the
> encoding where vowels are mapped to numbers as follows: (a=1, e=5,
> i=9, o=15, u=21, y=25 or 0). A vowel sequence is a maximal substring
> of consecutive vowels. A vowel product is the product of the vowel
> encoding of all vowels in a vowel sequence. The vowel product sum
> is the sum of the vowel product of all vowel sequences in the
> string.
> A letter in [AEIOUaeiou] is ... 阅读全帖 |
|
G****a
发帖数: 10208 | 17 Illumina, Inc. (NASDAQ:ILMN) today announced the launch of its TruGenome
Undiagnosed Disease Test provided by the CLIA-certified, CAP-accredited
Illumina Clinical Services Laboratory (ICSL). This new service uses whole
human genome sequencing to assist physicians in identifying the underlying
genetic cause of a rare or undiagnosed disease. By providing physicians with
more comprehensive information from a single test, the service enables a
broader understanding of the genetic basis for disease.
... 阅读全帖 |
|
发帖数: 1 | 18 CRISPR, /ˈkrɪspər/, is a family of DNA sequences in bacteria
that contains snippets of DNA from viruses that have attacked the bacterium.
These snippets are used by the bacterium to detect and destroy DNA from
further attacks by similar viruses. These sequences play a key role in a
bacterial defence system,[2] and form the basis of a genome editing
technology known as CRISPR/Cas9 that allows permanent modification of genes
within organisms.[3]
The CRISPR/Cas system is a prokaryoti... 阅读全帖 |
|
|
l*******w
发帖数: 61 | 20 应是如此。参见以下USGAAP原文（尤其是45-7和45-9两段）：
----- Under ASC 740-10 -----
> > > Deferred Tax Accounts Related to an Asset or Liability
45-7 [ A deferred tax liability or asset for a temporary difference that is
related to an asset or liability
shall be classified as current or noncurrent based on the classification of
the related asset or liability. [FAS 037,
paragraph 4, sequence 13.2.1.2.1] ]
45-8 [ A temporary difference is related to an asset or liability if
reduction [FAS 037, paragraph 4, sequence 1... 阅读全帖 |
|
c********e
发帖数: 598 | 21 madhadron: A farewell to bioinformatics
I’m leaving bioinformatics to go work at a software company with more
technically ept people and for a lot more money. This seems like an
opportune time to set forth my accumulated wisdom and thoughts on
bioinformatics.
My attitude towards the subject after all my work in it can probably be best
summarized thus: “Fuck you, bioinformatics. Eat shit and die.”
Bioinformatics is an attempt to make molecular biology relevant to reality.
All the molecular biolog... 阅读全帖 |
|
w*****m
发帖数: 414 | 22 丸子的这个算法有个小问题。如果你看我的原始数据描述就会发现，每一个id中的
sequence有时候
是重复的。比如说，对于同一个id，事件在2000年发生一次，sequence=1, 2001没有发
生，跳
过，2002年发生一次，sequence=1， 2003年没发生，再跳过；2004年又发生一次，
sequence
还是=1，2005年没有发生，再次跳过。
对于这个id,sequence=1出现三次。丸子直接做
proc univariate data=sum noprint;
class id sequence;
就有问题了。
proc sort data=sum;
by id sequence;
run;
proc univariate data=sum noprint;
class id sequence;
var x;
output out=report sum=sum mean=mean median=median std=std;
run; |
|
d****n
发帖数: 233 | 23 Since it's a circular sequence, we want to find the optimal start point in
this sequence, so that the LIS exists in it. we have two ways to solve it.
1. Start at arbitary element and form a sequence, repeat this sequence and
find the LIS from the new sequence of size 2N. of course, we can stop early
if a LIS with length N is find(if all the elements are the same in the
sequence). the complexity will be O(2N*Log(2N))
2. Start at arbitary element and form a sequence, find all the ending
eleme |
|
d****n
发帖数: 233 | 24 int longestZigZag(int sequence[], int num)
{
// arguments validation skipped
int s= 1;
while (s < num && sequence[s] == sequence[s-1])
s++;
if (s == num ) return 1;
int count = 2;
int increasing = (sequence[s]-sequence[s-1] > 0)?1:-1;
while (s < num)
{
if (increasing*(sequence[s]-sequence[s-1]) < 0)
{
++count;
increasing *= -1;
}
++s;
}
return count;
} |
|
k***s
发帖数: 277 | 25 受到wiki上的启发， http://en.wikipedia.org/wiki/Integer_partition
wiki上是用smallest addedn is k, 我改成with m's largest addend k
// k is the maximum number of integer partition;
// m is the num of k of integer partition.
void ip_helper(int n, int k, int m, std::deque sequence) {
if (n < k*m)
return;
if (k < 1)
return;
int i, j, r = n-k*m;
for (i=0; i
sequence.push_back(k);
if (r == 0) {
for (i=0; i<(int)sequence.size(); ++i)
std::c... 阅读全帖 |
|
i********f
发帖数: 206 | 26 帮朋友转发的
Position Description
The candidate will work with Ambry Genetics’ next-gen sequencing crew,
bioinformatics colleagues and IT staffs to develop and utilize programs to
collect, store, manage, analyze and report biological sequencing data;
assist bioinformatic data analysis and generation of deliverables of
genomics service projects; assist both clinical and genomic services
sequencing data storage and management.
Requirements
Candidates should have a Bachelor or Master's degree in Bioinfor... 阅读全帖 |
|
k**n
发帖数: 3989 | 27 靠，，毕业后就没看过这种东东。。。读题就晕了。。
A non-empty zero-indexed array A containing N different integers is given.
We are looking for the longest possible sequence built from elements of A, A
[B[0]], A[B[1]], ..., A[B[K]], satisfying the following conditions:
The sequence must be decreasing; that is, A[B[0]] > A[B[1]] > ... >
A[B[K]].
For any two consecutive elements of the sequence, A[B[I]] and A[B[I+
1]], all the elements of A between them must be smaller than them; that is,
for any J = MIN(B... 阅读全帖 |
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Z*****Z
发帖数: 723 | 28 The idea is to reconstruct the original tree while walking through it, using
the given sequences. We use pre-order sequence to traverse down, while usin
g post-order sequence to back out.
The reconstructed tree will be a general tree, meaning each internal node ca
n have arbitrary number of children. Also each node will have a parent point
er.
We need three pointers: treePtr, prePtr and postPtr that point to the curren
t tree node, pre-order sequence and post-order sequence, respectively. The r
... 阅读全帖 |
|
l*******s
发帖数: 7316 | 29 A_n = {n pairs of valid parentheses}
B_n = {321-avoiding permutations in S_n}
For any a_i in A_i, c(a_i) is defined as number of pairs of
parentheses in a_i which are not within any other parentheses.
For any a_n in A_n, (c(a_n,1),c(a_n,2),...c(a_n,n)) is called
construction sequence of a_n,
where a_n,i is sub-sequence of a_n made of of first i ")"s
and their corresponding "("s.
For example the construction sequence of ()(()) is (1,2,2)
For any b_i in B_i, c(b_i) is defined as maximum number ele... 阅读全帖 |
|
l*g
发帖数: 46 | 30 下面是我一堂课上的讲义
Repetive elements in query sequences can cause spurious database
matches. These sequences lead to artifically high alignment scores
with database sequences when they are acturally not related to databases
sequences. Alu repeats, low-complexity regions (LCRs) with short period
repeats or overrepresented residues. In fact, one half of protein
sequences in databases contain at least one LCR.
Reason: the LCR sequences do not fit the residue-by-residue sequence
conservation and therefore |
|
v***a
发帖数: 1242 | 31 谢谢。其实我现在的问题就是不知道该如何设计出clone全长coding sequence的primer
，因为sequence 1和sequence 2的cds也不尽相同，而且sequence 1只是partial cds，
无stop codon，而我针对sequence 2设计的primer又总是clone不到我要的gene（其实
我觉得sequence 1可信度较高，所以现在怀疑是不是sequence 2是不确切的）。我现在
想做的就是你的思路，但为难在primer上了。
PCR |
|
j*p
发帖数: 411 | 32 理想条件，假设library里面有n个rna，每个rna expression 为ni，假设fragments，
sequencing等都是random，那么理论上讲，每个rna被sequence到几率应该和rna的
expression level成正比。问题是有两个不同的rna pool，一个control，一个treat，
这两个sample中rna level 的distribution 不同，total number of rna copies 也不
同，假设两个sample拿去被sequencing的总量一样，并且都被sequence出来10M reads
，那么有可能，在control里面只sequence到expression level最大的1k个rna，其他的
rna reads=0，在treat里面，却sequence到了2k个rna。假设你说的rna在control里面
排第1001，有10个copy，在treat里面也排1001，有10个copy，用sequence，一个没表
达，一个有表达。如果用pcr，两个都有表达。
我认为，不应该是map的问题，the ... 阅读全帖 |
|
j*p
发帖数: 411 | 33 理想条件，假设library里面有n个rna，每个rna expression 为ni，假设fragments，
sequencing等都是random，那么理论上讲，每个rna被sequence到几率应该和rna的
expression level成正比。问题是有两个不同的rna pool，一个control，一个treat，
这两个sample中rna level 的distribution 不同，total number of rna copies 也不
同，假设两个sample拿去被sequencing的总量一样，并且都被sequence出来10M reads
，那么有可能，在control里面只sequence到expression level最大的1k个rna，其他的
rna reads=0，在treat里面，却sequence到了2k个rna。假设你说的rna在control里面
排第1001，有10个copy，在treat里面也排1001，有10个copy，用sequence，一个没表
达，一个有表达。如果用pcr，两个都有表达。
我认为，不应该是map的问题，the ... 阅读全帖 |
|
i********f
发帖数: 206 | 34 【 以下文字转载自 JobHunting 讨论区 】
发信人: iamicewolf (icewolf), 信区: JobHunting
标 题: 征Bioinformatics Analyst
发信站: BBS 未名空间站 (Thu Dec 1 12:05:05 2011, 美东)
帮朋友转发的
Position Description
The candidate will work with Ambry Genetics’ next-gen sequencing crew,
bioinformatics colleagues and IT staffs to develop and utilize programs to
collect, store, manage, analyze and report biological sequencing data;
assist bioinformatic data analysis and generation of deliverables of
genomics service projects; assist both cl... 阅读全帖 |
|
n******7
发帖数: 12463 | 35 情况大概是这样：我们做了很多cDNA clone，测序之后选取了一些进行了下游的实验，
主要是in vitro 的protein实验。我们鉴定了很多新的splicing isoform，其中有不少
有premature stop codon(可以是splicing 造成的，也可以使indel造成的）
现在我们想做一些quality control，去掉一些不靠谱的transcripts，于是出现了分歧
组里的大姐想法是要尽量跟已知的protein一致。把每条isoform对应的protein align
到已有的protein sequence上。如果整条protein基本align上去，即便使truncated
protein, 不管什么原因，都可以作为partial protein 保留。但是如果shifted frame
的amino acid sequence长到一定程度，那就认为这些protein sequence跟已有的
sequence太不一样，要除去
我完全明白大姐为什么那么想，因为我们实际test的是一些protein sequences
但是就我这部分工作，我需要关... 阅读全帖 |
|
f****b
发帖数: 2410 | 36 BIOINFORMATICS for Next Generation-Sequencing analysis @ ICBR of University of Florida
RESPONSIBILITIES:
• Provide high level bioinformatics support to molecular biologists
by applying information technology and statistics to genomic and proteomic
projects;
• Evaluate available bioinformatics tools for next generation-
sequencing data and prototype new methods and algorithms to enhance existing
analysis capabilities and pipelines;
• Provide computational support for in... 阅读全帖 |
|
l**********1
发帖数: 5204 | 37 单分子RNA-seq 测序仪器的错误率从15%减少到了不到0.1%。
pls refer :
美研究人员成功解码“学习能力”相关基因
translation from article:
Hybrid error correction and de novo assembly of single-molecule sequencing
reads
by
Koren S. et al. Nat Biotech (2012)
2012-07-18
据外媒报道，有US科学家称，他们组合了一条更完整的基因链条，这个基因链条可
以控制鹦鹉模仿主人和其他声音的能力。
研究者用一种新技术分解了鹦鹉的基因组中的某个区域，进行了单分子测序，并用
来自较早的DNA解码设备的数据对其进行了纠错。研究者还解码了来自玉米和细菌的难
以测序的基因物质，以此来证明他们新的排序方法。
单分子测序“在去年获得了大量宣传”，因为它产生了测序长读取，“使组装基因
组中的复杂部分变得更容易，”研究参与者之一，Duke大学的神经生物学家Erich
Jarvis说。
Jarvis对那些控制鹦鹉模仿能力的序列很感兴趣，因为它们能... 阅读全帖 |
|
u*********1
发帖数: 2518 | 38 我做ALS的，比如我们要identify一个新的ALS gene，可能需要screen几百甚至上千个
sample
当然我的问题和你的不同，因为这里的screen hundreds samples基本上还是用sanger
sequencing去测某几个位点（当然也在策划deep sequencing）；而且问题是ALS属于
complex disease，找到的基因都只可能是account for 1% of all samples
所以我好奇的是你这种疾病是不是monogenic disease；如果是的话就相对好办吧，哪
怕你只有三个sample，但assumption是所有的病人的致病机理是一样的，你先做着这三
个sample也总是可以发现点规律。当然我不知道甲基化，乙酰化的deep sequencing的
成本，但我相信总归算target sequencing，算算exome sequencing现在的成本降低的
厉害，所以我猜想你的这个也不会太贵吧？
做这种研究的思路我觉得一般都是用deep sequencing测序一部分的重要的样本，找到
一些有趣的candidate，你就... 阅读全帖 |
|
G******O
发帖数: 189 | 39 Deepsequencing结果没有找到？
Casey Bergman：
I was also unable to find the data associated with this paper. As such, I
have written to Nature to ask them to confirm whether the NGS data from
Obokata et al has indeed been deposited in a public database, as is required
by their “Availability of data and materials” policy. My letter is as
follows and was posted today 13-2-2014.
Dear Editor -
It has come to my attention that a recent paper published in your journal
that reports next-generation sequencing da... 阅读全帖 |
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发帖数: 1 | 40 For those who can not access the link
I copied the content here:
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
NgAgo
15 posts by 9 authors
Davide Seruggia
Jun 23
Hi,
I tried a couple of transfections with the NgAgo plasmid from Addgene and
the 5'P oligo against GFP from the paper, but I did not see a dramatic
reduction in GFP+ as seen using Cas9 and a GFP sgRNA.
Anybody tried as well and came to the same (dead) end?
Davide
k*****[email protected]
Jun 24
Hi, Davide,
I tried th... 阅读全帖 |
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m*********D
发帖数: 1727 | 41 （CRISPR的背景资料请参阅Feng Zhang的“Genome engineering using the CRISPR-
Cas9 system”，Nature Protocols 8, 2281–2308 (2013)）
背景
我们是作一个与激素相关的肿瘤的。这个肿瘤的治疗方法已经相当完善，就是让身体里
不能生产激素或用一个小分子化合物抢占激素受体上激素的结合部位。但多年治疗后，
会有相当比例的病人产生抗药性。这个抗药性机理相当复杂。我们实验室一直致力于筛
选和现有药物不同的机理的小分子化合物，包括受体和DNA的结合或其他与受体相关的
pathway的inhibitors。前几年成功地筛选出了几个不同机理的化合物，个别在细胞水
平达到了nM的级别，动物测试也在10 mg/kg的水平。
最近在这个领域对抗药性肿瘤的研究发现，部分病人的抗药性是因为受体激素结合部位
发生了突变。一个或几个氨基酸的突变就能导致肿瘤细胞的生长不能被现有药物抑制。
所以，我们很想知道我们筛选出来的化合物对这些突变受体是不是也起作用。理论上来
说，我们的化合物不和激素竞争，突变不会影响我们化合物的效果。但只... 阅读全帖 |
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c*********d
发帖数: 9770 | 43 荒猫牛不耕微信号laoniu003005
功能介绍
好文分享
来源：荒猫之舞
作者：荒猫
中美贸易战告一段落，毫不意外地，咱们又赢了。不过与以往不同的是，这次美国也赢
了，是双赢。据报道，咱们答应了美国减少贸易逆差的要求，所以美国赢了；而因为要
减少贸易逆差，中方将大量增加自美购买商品和服务，这样可以满足咱们不断增长的消
费需求和促进高质量经济发展，所以咱们也赢了。
但让我感觉不可思议的是，这么好的事情，为什么一直不做呢？为什么要在美国政府的
逼迫下去做呢？是因为没有想到？那岂不是说那些人能力低下？是因为想到了而不做，
那岂不是证明了那些人很坏？
而且在美国高调启动贸易战之初，咱们是抱着多大的决心，宁为玉碎不为瓦全，一定要
打赢的，一定要粉碎美国人阻挡咱们大国崛起的阴谋的。当美国提出500亿的清单时，
咱们针锋相对；当美国提出1000亿的清单时，咱们继续应战；而当美国把清单的总价提
高到2000亿的时候，一夜之间，美国就不是阻挡咱们大国崛起，而是有利于咱们大国崛
起了。美国的要求原来是有利于咱们的发展，而不是遏制咱们的发展了。我恍然大悟：
原来以前的针锋相对，是用的激将法，是为了刺激美... 阅读全帖 |
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M********a
发帖数: 10 | 44 默沙东(美国默克), 世界500强，全球第二大制药公司。
Merck & Co., Inc., known as MSD outside North America, is committed to
scientific research in China. MSD China R&D is proud to announce the launch
of the China Postdoctoral program offering Chinese postdoctoral researchers
a new path to the forefront of their research (in the areas of Biostatistics
, Bioinformatics and health/medical informatics). The MSD China R&D post-doc
will be stationed in the MSD Beijing site and will conduct research with
scientists in MSD Chi... 阅读全帖 |
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g**********y
发帖数: 14569 | 45 写了个简化版的：
public class Gloomyturkey implements IMilestone {
private int N;
private int M;
private int[] sequence;
private int[] answer;
private HashMap map;
private boolean[] used;
private int[] sum;
private boolean found;
public int[] findMilestones(int[] segs) {
M = segs.length;
sum = segs;
calcN();
initMap(segs);
used = new boolean[M];
found = false;
sequence = new int[N];
answer = new int[N];
assign(0, M-1);
assign(1, M-2);
dfs(2);
return ... 阅读全帖 |
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g**********y
发帖数: 14569 | 46 写了个简化版的：
public class Gloomyturkey implements IMilestone {
private int N;
private int M;
private int[] sequence;
private int[] answer;
private HashMap map;
private boolean[] used;
private int[] sum;
private boolean found;
public int[] findMilestones(int[] segs) {
M = segs.length;
sum = segs;
calcN();
initMap(segs);
used = new boolean[M];
found = false;
sequence = new int[N];
answer = new int[N];
assign(0, M-1);
assign(1, M-2);
dfs(2);
return ... 阅读全帖 |
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l*****a
发帖数: 14598 | 47 if so, how do u push_front and pop_front
Double ended queue
deque (usually pronounced like "deck") is an irregular acronym of double-
ended queue. Double-ended queues are a kind of sequence container. As such,
their elements are ordered following a strict linear sequence.
Deques may be implemented by specific libraries in different ways, but in
all cases they allow for the individual elements to be accessed through
random access iterators, with storage always handled automatically (
expanding an... 阅读全帖 |
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o*q
发帖数: 630 | 48 # Title Editorial Acceptance Difficulty Frequency
1
Two Sum 28.3% Easy
292
Nim Game 54.4% Easy
344
Reverse String 57.3% Easy
136
Single Number 52.2% Easy
2
Add Two Numbers 25.6% Medium
371
Sum of Two Integers 51.6% Easy
4
Median of Two Sorted Arrays
20.4% Hard
6
ZigZag Conversion 25.6% Easy
13
Roman to Integer 42.7% Easy
237
... 阅读全帖 |
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j*x
发帖数: 30 | 49 Postdoc Position At The Methodist Hospital Research Institute
Department of Systems Medicine And Bioengineering (SMAB) at The Methodist
Hospital Research Institute seeks an individual to support Next Generation
Sequencing analysis and software development for genomics research.
SMAB is well funded by NIH/NCI and CPRIT. More information please refer to
the website: http://www.methodisthealth.com/smb
Responsibilities:
• Testing and understanding of the established NGS sequencing
pipelines... 阅读全帖 |
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M********a
发帖数: 10 | 50 默沙东(美国默克), 世界500强，全球第二大制药公司。
Merck & Co., Inc., known as MSD outside North America, is committed to
scientific research in China. MSD China R&D is proud to announce the launch
of the China Postdoctoral program offering Chinese postdoctoral researchers
a new path to the forefront of their research (in the areas of Biostatistics
, Bioinformatics and health/medical informatics). The MSD China R&D post-doc
will be stationed in the MSD Beijing site and will conduct research with
scientists in MSD Chi... 阅读全帖 |
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