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w*******y
发帖数: 60932 | 3 Paracord 550lb Type III 7 Strand 100' Hanks
Every Color available only $5 per 100' Hank - Cant find it cheaper anywhere
Made from a US Government Contractor
550lb Min Tested
Link:
http://www.paracordmaster.com/index.php/commercial-550-paracord
They also offer Flat Rate $6 Shipping with no minimums or maximums
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b*******e
发帖数: 6920 | 6 Friday, February 10, 2012
Date Time Event
Location
2/10 5pm Krewe da’ Yaga’s Balcony Party Dargan &
Tobyn 23rd and Strand
2/10 5pm Mardi Gras Carnival 3400 Seawall Blvd.
2/10 5pm Mardi Gras! Galveston Downtown Galveston
2/10 5pm Nakia After Show at Bud Light Balcony Party
Trumpets 23rd and Strand
2/10 5pm PARADE: Party Gras! ... 阅读全帖 |
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b*******e
发帖数: 6920 | 7 Friday, March 4, 2011
3/4 5pm Krewe Babalu Balcony Party Dargan & Tobyn Bldg
23rd & Strand
3/4 5pm Mardi Gras! Galveston Downtown Galveston
3/4 5pm Official Mardi Gras! Galveston Balcony Party Dargan & Tobyn
East 23rd and Strand
3/4 5pm Seawall Carnival 3400 Seawall Blvd.
3/4 6pm Z Krewe Zestival I Trumpets Building
2215 Strand
3/4 7pm Dr. Michael White Jazz Quartet The Tremont House
2300 Mechanic
3/4 7pm Krewe Maximilian Bead Party ... 阅读全帖 |
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s******9
发帖数: 283 | 8 NCBI protein database一般都有标注吧。
FEATURES Location/Qualifiers
source 1..503
/organism="Homo sapiens"
/db_xref="taxon:9606"
gene 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
Protein 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
... 阅读全帖 |
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b*******e
发帖数: 6920 | 9 Saturday, February 26, 2011
2/26 11am Mardi Gras! Galveston Downtown Galveston
2/26 11am Seawall Carnival 3400 Seawall Blvd.
2/26 12pm BAND: Mambo Jazz Kings Budweiser Stage 23rd & Strand
2/26 12pm Fish Tales Balcony Party Fish Tales 25th & Seawall Blvd.
2/26 12pm Mystic Krewe of Aquarius Mardi Gras Kickoff Parade 1400
Seawall Blvd.
2/26 12pm Official Mardi Gras! Galveston Balcony Party Jockusch
Building 21st & Strand
2/26 12pm Official... 阅读全帖 |
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b*******e
发帖数: 6920 | 10 Mardi Gras! Galveston Parade Schedule
Texas’ Largest Mardi Gras Celebration Comes With Parades, Family Gras and
More!
[January 10, 2013] - The 102nd celebration of Mardi Gras! Galveston will
take place February 1-12, 2013 with 24 parades, several of which are new.
The majority of parades will be held in Galveston's downtown Mardi Gras
entertainment district while others will travel along the beachfront on
Seawall Boulevard.
Friday, February 1, 2013
Party Gras! Parade, 5 p.m. (Entertainment Distr... 阅读全帖 |
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a****0
发帖数: 548 | 11 我尽管一直想动手学做fashion配件，但没有时间。女儿最近一直在嚷嚷，要我给她买
手工艺配件做耳环
和项链。终于下定决心，在firemountain上下了单。由于是100%的新手，不知道所买的
配件合适否，
请各位高手指导下(非常感谢！！！）：
Item Number: Item Description: Quantity: Item Total:
H20-1214WR Wire, 12Kt gold-filled, half-hard, round, 26 gauge. Sold
per pkg of 5 feet. 3 $14.88
H20-1422FN Earwire, 14Kt gold-filled, 13mm fishhook with open loop, 21
gauge. Sold per pkg of 5 pairs. 1 $7.21
H20-1608TL Pliers set, carbon steel, 4-1/2 inches long, with roll-up
case. Sold per se... 阅读全帖 |
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b*******e
发帖数: 6920 | 12 说明：我也是第一次参加，道听途说了一些——
downtown到时候是要票的，一个人几块钱，进去后有表演有小摊有花车
seawall的游行是免费的，区域从21街到61街，会有花车扔珠子什么的。肯定会people
moutain people sea啦，大家注意安全
Balcony Party 据说阿，据说要做好准备看到一些放荡的人，纯洁的小孩不要带去。当
然如果你没看到放荡的人也不要来问我为什么没看到=，=
Friday, February 25, 2011
2/25 5pm Mardi Gras! Galveston
Downtown Galveston
2/25 5pm Official Mardi Gras! Galveston Balcony Party Jockusch
Building 21st & Strand 以及 2300 Strand
2/25 5pm Seawall Carnival 3400 Seawall Blvd.
2/25 5pm Stars on the Brazos Krewe Balcony Par... 阅读全帖 |
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w****w
发帖数: 14828 | 13 10年前看得不知是什麼了，不過就手wiki來的如下，有些太長就不copy了
The partially double-stranded DNA is rendered fully double-stranded by
completion of the (+) sense strand and removal of a protein molecule from
the (-) sense strand and a short sequence of RNA from the (+) sense strand.
Non-coding bases are removed from the ends of the (-) sense strand and the
ends are rejoined. There are four known genes encoded by the genome, called
C, X, P, and S. The core protein is coded for by gene C (HBcAg), and its
start codon is p |
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l****z
发帖数: 29846 | 14 98% of Stories Ignore That Ice-bound Ship Was On Global Warming Mission
A group of climate change scientists were rescued by helicopter Jan. 2,
after being stranded in the ice since Christmas morning. But the majority of
the broadcast networks’ reports about the ice-locked climate researchers
never mentioned climate change.
The Russian ship, Akademic Shokalskiy, was stranded in the ice while on a
climate change research expedition, yet nearly 98 percent of network news
reports about the stranded... 阅读全帖 |
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b*******e
发帖数: 6920 | 15 Sunday, February 27, 2011
2/27 11am Mardi Gras! Galveston Downtown Galveston
2/27 11am Merchant Walkabout 2300 Strand
2/27 11am New Orleans Style Brunch Hotel Galvez 2024 Seawall Blvd.
2/27 11am Seawall Carnival 3400 Seawall Blvd.
2/27 12pm BAND: Bilge Pumps Budweiser Stage 23rd & Strand
2/27 1:30pm “Sunshine Kids” Parade 2300 Strand
2/27 2:45pm BAND: Kevin Anthony Budweiser Stage 23rd & Strand
2/27 5pm BAND: Spencer Carter & T... 阅读全帖 |
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d********f
发帖数: 43471 | 16 【 以下文字转载自 USANews 讨论区 】
发信人: lczlcz (lcz), 信区: USANews
标 题: 那些被南极冰堵住的人原来是去研究全球暖化的,哈哈
发信站: BBS 未名空间站 (Fri Jan 3 15:28:53 2014, 美东)
98% of Stories Ignore That Ice-bound Ship Was On Global Warming Mission
A group of climate change scientists were rescued by helicopter Jan. 2,
after being stranded in the ice since Christmas morning. But the majority of
the broadcast networks’ reports about the ice-locked climate researchers
never mentioned climate change.
The Russian ship, Akademic Shokalskiy, was... 阅读全帖 |
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T*****n
发帖数: 2456 | 17 第一篇 一个基要派出身的，圣公会司铎转到天主教的神父写的
http://www.catholic.com/magazine/articles/peter%E2%80%99s-autho
Peter’s Authority More Solid Than a Rock
By: Fr. Dwight Longenecker
When I was in the Bible doctrine class at Bob Jones University, one of the
verses we had to memorize was Matthew 16:18: "I tell you that you are Peter,
and on this rock I will build my church and the gates of hell will not
prevail against it."
A Catholic student might memorize this verse to prove his beliefs about the
papacy. We learned ... 阅读全帖 |
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f*******e
发帖数: 628 | 18 都有错误，所以 illumina 454 啥的都要事先 sample amplification。这个 nanopore
既然号称不需要 sample prep, 就需要解决单分子测序的 error 问题。当然如果
sample 本来就是 amplicon 就无所谓了。
哪个文章？他们网站上说是有两种 mode，其中一种是用 exonuclease 的。我理解那个
strand sequencing 不用 nuclease，但是具体怎么 把 double strand 弄成 single
strand，然后 single strand 通过了之后如何 recover 不是太明白。
我没以为是单细胞测序，所以才说 error 要通过 coverage 来解决。而 PacBio 的某
个解决 error 方法是循环的重复测某一个单分子，这一点 Nanopore 好像做不到，因
为我的理解是某个 strand 通过测序之后被重复测的几率很低很低。这个 error 的问
题，特别在 sample 本身很少的情况下，比如一个 finger stick 的血样，不能解决的
话就是个问题。 |
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f*****h
发帖数: 228 | 19 给你贴了吧，大家想用都可以
#!/usr/bin/perl -w
#############################################
#Author: Jiang Li
#email: r**********[email protected]
#Creat Time: Tue 23 Oct 2012 01:37:54 PM CDT
#Vanderbilt Center for Quantitative Sciences
#############################################
use strict;
use warnings;
=pod
=head1 SYNOPSIS
Given a genbank format file (.gb), parse its feature parts (mRNA feature to
get exon regions) to get information like transcript id, gene name, etc.,
and store the result in gtf format
=he... 阅读全帖 |
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l**********1
发帖数: 5204 | 20 Pls check,
>This underlines the importance of identifying the presence and
understanding the function of these antisense non-coding RNAs. The
information concerning strand ori-gin is often lost during conventional RNA-
Seq; capturing this information would substantially increase the worth of
any RNA-Seq experiment. By manipulating the input cDNA during the template
preparation stage it is possible to retain this vital information. This
forms the basis of strand-specific RNA-Seq. With an ability ... 阅读全帖 |
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s****l
发帖数: 10462 | 21 有人愿意讨论这里面提及的测序技术吗？优缺点，可行性，第一手经验等等
这里面谁现在是赢家很清楚，但是可见的将来和更远的将来谁会赢？
http://cen.acs.org/articles/92/i33/Next-Gen-Sequencing-Numbers-
全文拷贝如下
试试看图能不能贴上
/1407973177171.jpg
/1408028636124.jpg
OVER STORY
Next-Gen Sequencing Is A Numbers Game
As technical and cost barriers fall, instrument firms move their systems
into research and clinical markets
By Ann M. Thayer
Department: Business
Keywords: instrumentation, gene sequencing, diagnostics, genomics, cancer
[+]Enlarge
09233-cover-Openercxd_17647969-690
A... 阅读全帖 |
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z*f
发帖数: 27 | 22 From Wiki:
Using the Beer-Lambert Law it is possible to relate the amount of light
absorbed to the concentration of the absorbing molecule. At a wavelength of
260 nm, the average extinction coefficient for double-stranded DNA is 0.020
(μg/ml)−1 cm−1, for single-stranded DNA it is 0.027 (μg/ml)&#
8722;1 cm−1, for single-stranded RNA it is 0.025 (μg/ml)−1 cm&#
8722;1 and for short single-stranded oligonucleotides it is dependent on the
length and base composition. Thus, an ... 阅读全帖 |
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t******l
发帖数: 10908 | 23 我觉得还有一种可能，是进化的时候，DNA 的两个拷贝机制是天生就是不对称的。。。
也就是好比 leading strand 那条是原版，lagging strand 那条是手抄本。
而在最初进化的时候，存在可能就啊 leading strand 那条原版要保持精确。。。而
lagging strand 那条就是看造化，运气不好就是 expendable，就好比产卵千枚，总有
存活下来的。。。后来发现产那么多卵也不是个事儿，就不断给 service pack
1234567，但换汤不换药，还是补丁，进化不够完全。
: 你这个类别不恰当，右撇子占大多数时左撇子应当迁就右撇子，但当左撇子右撇
子一样
: 多时确实有道理分开设备，而不是让一类承受沉重的负担去迁就另一类。
: 在dna复制这个问题上， 35与53是相同数目的。
: 一套右
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t******l
发帖数: 10908 | 24 如果按我专利律师上面的帖子的话，那其实是有两种 lagging strand 原因的假说。。
。一个是异步电机假说，另一个是手抄本产卵千枚假说。
但这两个假说在 lagging strand 修修补补杂碎的预测方面是不一样的。。。假设我们
能看到短 DNA 的原古原始原始生物的话：
那异步电机假说如果成立的话，那些 lagging strand 修修补补的杂碎的主干应该一开
始就有，如果不给力的话就根本无法复制，千分之一的正确率都不会有。
但如果手抄本产卵千枚假说成立的话，那么 lagging strand 即使没有那些杂碎，也应
该能够复制，也就是正确率低到千分之一，游离 DNA 单链到处像星光那样跪求内射成
双旋。。。而那时因为没有竞争对手，三千年才分裂一次也不是个事儿。
: 你这个类别不恰当，右撇子占大多数时左撇子应当迁就右撇子，但当左撇子右撇
子一样
: 多时确实有道理分开设备，而不是让一类承受沉重的负担去迁就另一类。
: 在dna复制这个问题上， 35与53是相同数目的。
: 一套右
|
|
发帖数: 1 | 25 实话说，我年轻时吃过狗肉，但后来绝对不吃了，而且很讨厌吃狗肉的行为。
----------------
我正在看的剧:
行尸之惧 第四季
https://daxiv.com/sc/detail/5b84744de401aa0008c20b4f
观众和媒体对于本季的关注，几乎全都放在Morgan Jones（Lennie James）这个角色
身上毕竟这个角色起到桥接该剧和母剧《行尸走肉》（The Walking Dead）的重要作用
，也是《行尸走肉》和《行尸危途》开播以来第一次出现情节交叉的情况。因为《行尸
危途》的故事时间要比《行尸走肉》早很多，因此观众在《行尸危途》中看到的Morgan
是「过去的Morgan」《行尸走肉》首集中的Morgan，甚至更早。在遇到Rick等人之前，
Morgan有过另外的朋友、另外的敌人和另外的威胁。@www.airuib.com
第四季的故事地点主要设定在德州，故事时间可能会发生一定幅度的时间跳跃。Alicia
Clark、Victor Strand和Luciana Galvez都还活着。其中Luciana已经消失了很长一段
时间（这是第三季Lu... 阅读全帖 |
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q******3
发帖数: 622 | 26 estimated printed pages | use the edit tools to save paper and ink!
Busted toilets, hot rooms, headaches after fire strands cruise ship in Gulf
By CNN Staff
updated 8:33 AM EST, Tue February 12, 2013
CNN.com
(CNN) -- Passengers on a Carnival cruise ship drifting in the Gulf of Mexico
aren't getting the vacation they expected -- sleeping on its decks, making
do with a few working toilets, and doing what they can to get food -- all
due to a weekend engine fire left the vessel dead in the water.... 阅读全帖 |
|
发帖数: 1 | 27 Single stranded DNA is much more difficult compared to double-stranded
plasmid DNA in terms of transformation.
Try these single stranded DNA transformation tricks.
1) transformation done at a low pH of ~6. Low pH significantly reduces the
nuclease activity of cutting single stranded DNA.
2) increase Mg++ to 1 mM. Mg is a conformation stabilizer of DNA and RNA.
3) No EDTA in the transformation system because it deprives Mg++ from the
buffer.
Han is a seasoned scientist with expertise on nucleic ... 阅读全帖 |
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n*****e
发帖数: 43 | 28 There are two very important reports in recent Cell discussing how to design
potent siRNA.One is from Zamore lab, the other from Amgen company. Basically,
the rule is to put a weak base pair, for example A-T or even mismatch with
target, at the 5'-end of the antisense strand of siRNA. Such a design
faciliates RISC selecting the antisense strand as guide strand, instead of
sense strand. Most of previous failed siRNA sequences follow the opposite of
rule. Given this discovery, other considerations |
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h******y
发帖数: 351 | 29 请问大家在设计shRNA的时候，target靶序列的antisense应该放在loop的哪边？前面？
后面？还是
无所谓？
对于shRNA转变成siRNA，请问下面所示的两种情况，哪种（loop在双链的左边或者在双
链的右边）形
式更容易与Dicer结合，从而被切割成siRNA？
/￣\
5'-sense-----strand－ | loop
3'-antisense-strand－\_/
/￣\
loop | -sense-----strand－3'
\_/-antisense-strand－5'
我看到很多人把antisense放在loop的后面，很多文献上也是这样示意的，但是
Genscript的人告诉
我放在哪边都无所谓。请问是这样吗？
多谢。 |
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s******y
发帖数: 28562 | 30 FT! FT! FT!
Of course you cannot ligate the single strand DNA like this way! Because
there is NOTHING to hold these two fragments together to allow the ligase to
act.
If you really want to ligate the single strand DNA, at least you should use
some kind of carrier system like BSA or other stuffs like a non-specific DNA
-binding protein.
If you choose to ligate from the double strand DNA, you can of course break
it down to single strand by incubating them on high temperature with
denaturing reagen |
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G***G
发帖数: 16778 | 31 i am asking some questions about the principle of pyrosequencing.
there are four types of deoxynucleotide triphosphates (dNTP).
each time one of them will be incorporated with the DNA strand.
but we don't know what type of nucleotide is in the DNA strand.
My question is the following.
1) Do we add all the four dNTPs to the reaction each time?
by 'each time' I mean each position of unknown nucleotide in the DNA strand.
2) or we add dATP, then dGTP, then dCTP, then dTTP each time?
3) do we need to... 阅读全帖 |
|
u*****8
发帖数: 180 | 32 早上读杂志看到的。
http://www.economist.com/node/21548488
In a paper published last year in the journal Science, Dr Winfree and his
colleague Lulu Qian described the use of strand-displacement cascades to
build circuits of increasing complexity, culminating in a circuit made of 74
different DNA strands (pictured) that was capable of calculating the square
roots of four-digit binary numbers. Together with their colleague Jehoshua
Bruck, they then built a tiny neural network, made up of four interconnected... 阅读全帖 |
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r*****t
发帖数: 4793 | 33 Nature. 2012 Mar 14;483(7390):434-8. doi: 10.1038/nature10895.
Intrinsic coupling of lagging-strand synthesis to chromatin assembly.
Smith DJ, Whitehouse I.
Source
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York
Avenue, New York, New York 10065, USA.
Abstract
Fifty per cent of the genome is discontinuously replicated on the lagging
strand as Okazaki fragments. Eukaryotic Okazaki fragments remain poorly
characterized and, because nucleosomes are rapidly deposited on n... 阅读全帖 |
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m*********D
发帖数: 1727 | 34 哦，我这个是template里就有两种：wt/mutant。PCR后产物一起digestion，指望
mutant变成两条带，从gel上纯化回收seq. 没切的band也从gel上回收后送seq。如果酶
切完全，又没有一条strand是mutant/一条strand是wt的DNA（我想这种DNA酶切不了）
，我就省了好多事呢。结果seq出来，在mutation区间有很多杂峰，就怀疑要么酶切不
完全或一条strand是mutant/一条strand是wt的DNA存在了。
再切一次，不行就只好克隆了。：（。
谢谢！
sanger |
|
m****a
发帖数: 270 | 35 【 以下文字转载自 WaterWorld 讨论区 】
发信人: gluestic (), 信区: WaterWorld
标 题: please transfer to Biology. RE:Ng-Ago 重复
发信站: BBS 未名空间站 (Tue Jun 28 19:07:03 2016, 美东)
Single stranded DNA is much more difficult compared to double-stranded
plasmid DNA in terms of transformation.
Try these single stranded DNA transformation tricks.
1) transformation done at a low pH of ~6. Low pH significantly reduces the
nuclease activity of cutting single stranded DNA.
2) increase Mg++ to 1 mM. Mg is a conformation stabilizer... 阅读全帖 |
|
F****8
发帖数: 289 | 36 您是韩老师吗？
这是你上月的帖子。
Single stranded DNA is much more difficult compared to double-stranded
plasmid DNA in terms of transformation.
Try these single stranded DNA transformation tricks.
1) transformation done at a low pH of ~6. Low pH significantly reduces the
nuclease activity of cutting single stranded DNA.
2) increase Mg++ to 1 mM. Mg is a conformation stabilizer of DNA and RNA.
3) No EDTA in the transformation system because it deprives Mg++ from the
buffer.
Han is a seasoned scientist with exp... 阅读全帖 |
|
F****8
发帖数: 289 | 37 这个 protocol 和 gluestic 六月28号发的三个tricks 中的两个能对得上。
看来 gluestic 和春雨有很近的联系。
下面是他当时的帖子：
Single stranded DNA is much more difficult compared to double-stranded
plasmid DNA in terms of transformation.
Try these single stranded DNA transformation tricks.
1) transformation done at a low pH of ~6. Low pH significantly reduces the
nuclease activity of cutting single stranded DNA.
2) increase Mg++ to 1 mM. Mg is a conformation stabilizer of DNA and RNA.
3) No EDTA in the transformation system because it de... 阅读全帖 |
|
w*******1
发帖数: 1789 | 38 太谢谢你的理解了。就是这样的情况啊。。
我是order 的single strand, 然后把两个strand给anneal到一起，买来的那些single
strand都好办，能测UV-Vis确定 浓度，然后 根据这个浓度找到double strand的浓度
，但这个里面肯定会有误差啊。所以老板让确定准确的浓度。。要用"chemical method
", 这是他原话。。
我以前做超分子的，根本不懂生物的东西啊。。。 |
|
w********h
发帖数: 12367 | 39 Italian physics professor Enzo Di Fabrizio captures twisted ladder that
props up life
DNA's double-helix structure is on display for the first time in this
electron microscope photograph of a small bundle of DNA strands.
Fifty-nine years after James Watson and Francis Crick deduced the double-
helix structure of DNA, a scientist has captured the first direct photograph
of the twisted ladder that props up life.
Enzo Di Fabrizio, a physics professor at Magna Graecia University in
Catanzaro, Italy,... 阅读全帖 |
|
r*******r
发帖数: 259 | 40 The world's rarest whale has been spotted for the first time, in New Zealand
, where two of the whales stranded themselves.
The two spade-toothed beaked whales, a mother and calf, stranded and died on
Opape Beach on the North Island of New Zealand, in December 2010. The
mother was 17 feet (5.3 meters) long and the calf was 11 feet (3.5 m) long.
A report describing the whales and the analysis of their DNA appears in the
Nov. 6 issue of the journal Current Biology.
"Up until now, all we have known... 阅读全帖 |
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D*********t
发帖数: 370 | 41 "About RNA polymerase II colliding, according to their data, the reverse
transcription not only happened in the promoter, but also coding region and
3’-UTR. If these bidirectional transcriptions happened in one cell, this
collide is unavoidable, I guess."
Pol II moves only in one direction on one strand. It can transcribe from
different strands – if the sense transcript is produced from the top strand
,
the antisense will be from the bottom strand. So there won't be any
collision.
in
S
question |
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a****o
发帖数: 1786 | 42 考古呀
Although RNA polymerase only use one strand as template during transcription
, both strands get into RNA polymerase and separate around Mg ++, so
collision is inevitable for head-to-head transcbing RNA polymerases.It is
not like DNA polymerase, which only take one strand of DNA into its active
center.
and
strand |
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C*I
发帖数: 4736 | 43 这是一个minutes by minutes story, 很有意思，有兴趣的可以看一看,长点见识。说
不定这事什么时候你真能遇上。
其中说了一个小伙子，在田纳西一个社区学院读书，回到土耳其去看他的新婚妻子，结
果就回不来了。他的妻子是乌兹别克斯坦人。 昨天突然听到消息说法官临时限制了总
统令后，就又赶紧花了1000多块钱买了机票折回来了。
WASHINGTON (AP) — The Latest on the reaction to a court order blocking U.S.
President Donald Trump's ban on travelers and immigrants from seven
predominantly Muslim countries (all times Eastern):
1:30 p.m.
The Justice Department has appealed a judge's order blocking his travel ban
at the 9th U.S. Circuit Court of Appeals in Sa... 阅读全帖 |
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t******l
发帖数: 10908 | 44 对于第三种情况，像我这个专利律师，从专利逻辑上就能看出来，如果反向酶存在并且
同时被使用，那导致 lagging strand 和 leading strand 一样都是连续生成，而两条
连续生成，那势必要在微观上严格同步，否则的话就要把 DNA 复制器给二马分尸了。
。。但 what if 海森堡同志说，这个两条车流的微观速度涨落无法严格保持一致。。
。于是左旋的立马先下手为强，抢下连续流的 TCP 端口 leading strand，而右旋的就
后下手遭殃，也只有断断续续然后接收方重新缓存排序的断续流 IP 端口可用了。
当然这还是专利合同律师的看法，但至少逻辑上构成全集不是？
: 你还是没看懂我说的意思。。。我的意思是，一种情况是反向酶不可能存在，另
一种情
: 况是反向酶被使用（我这里特地用了马工 formal logics 避免二义性），但这
两种情
: 况，逻辑学上不构成全集啊。。。还有第三种情况，是反向酶可能存在，但无法
跟正向
: 酶一起同时被 DNA 复制机所使用？是不是可能？
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T**********2
发帖数: 38 | 47
粘贴几段：
1。 First, the ECO transmission should not be the default when you turn the
car on. It goes thru the gears too quickly, and hesitates when you need to
accelerate. 2nd - they need to smooth the transition from 1st to 2nd gear at
slow speeds in the normal transmission mode. The car jumps into 2nd, even
if you have released the accelerator, and slows down too quickly from 2nd to
1st when braking. All in - the transmission is horrible - maybe it's best
to start over. They also need to improve ... 阅读全帖 |
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r********e
发帖数: 1686 | 48 5分钟叉烧包，中种全卖土司
叉烧，可能不来美国我这个西北人怎么也想不起来做这个东东。 第一次做，就用了李
锦记的叉烧酱腌制里脊肉24小时，然后上烤架（不要烤盘上）400度中间翻面烤了大概
40分钟。叉烧酱很拈，但是也不要用水稀释按摩摸均匀就好。 感觉味道还是不太进去
，总结原因是腌制的时候没有翻面，以及肉表面的白膜没有去掉。
剩下的叉烧就做叉烧包了。面团就是5分钟面团，整形都一样包成个圆形就好了。 上次
有mm问整形过程，我描述了半天也没说清楚，这次就发过过程比较形象。包陷进去的话
皮最好是中间厚点，因为后来要用虎口往里收为了让表面的光滑。其实这就和江浙一带
做带陷的馒头一样，只不过我们是进烤箱烤的。
5分钟面包做多了，渐渐多了些对于面团的认识。摘抄一段5 minutes artisan bread
里面对于no knead bread 的原理描述：
'
gluten is strengthened when the proteins align themselves into strands after
water is added. resilient,stretchy, gas-... 阅读全帖 |
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a**i
发帖数: 608 | 49 1 Purchase lights specifically suitable for outdoor use. They should be
labeled as outdoor lights and have a small, circular label that says "UL" or
"ETL."
2 Plug your Christmas lights into a ground-fault circuit interrupter, or
GFCI. A GFCI is a device that automatically detects short circuits and shuts
off the current. They can be recognized by two buttons labeled "test" and "
reset" built into the plug. If you do not have a GFCI, you can buy a
portable one and plug it into a wall socket.
3 Ch... 阅读全帖 |
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s*******w
发帖数: 2257 | 50 美国 9.11事件 发生时的另一航班 - - 达美15
2015-05-22 20:03:29
生活里天天充滿着美好充盈着愛。
我是達美航空公司15號班機的機組人員，2001年的9月11日從德國的法蘭克福機場飛越
大西洋，目的地是美國的亞特蘭大。
忽然，機艙內的布簾都拉了起來，我被叫到駕駛艙去見機長。在那兒，幾個機組人員都
面露嚴肅。機長給我一張紙條，這是從亞特蘭大機場辦公室發出來的訊息，上面寫著：
「所有私人航空公司飛往美國的航道全部關閉，請盡速降落在最近的機場，並請告知你
的降落目的地。」
沒有一個人對此訊息有隻字片語，我們都知道事態的嚴重性。我們需要立刻找到一個陸
地降落，機長決定最近的機場是離我們400英里遠的甘德, 紐芬蘭。
機長要求加拿大航空交通控制中心核准路線的改變，我們沒有被質疑而獲得即刻的核准
，後來我們才知道為何我們的要求毫不遲疑的被核准。
當我們正準備降落時，另一個訊息從亞特蘭大傳來，告訴我們在紐約地區有恐怖行動。
幾分鐘後，我們知道是劫機事件。
尚未降落前，我們決定跟乘客撒個白謊，說是一個小小的機械問題需要降落在最近的機
場-甘德, 紐芬蘭，做個檢查。
我們答應... 阅读全帖 |
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