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q******g
发帖数: 210
1
来自主题: Chemistry版 - 大妈是有钱任性吗.
快要奔四十的大妈了,有两娃,四岁和七岁。当初本科后追随老公来美三流学校读有机
博士。所有课程都修完,博士资格也过了。第一个老板没拿到终生教职在我读满第二年
的时候走了,所以被迫换组,换方向 (生物化学),又读了三年。第二任老板又转校
。当时有三个选择:1.跟着走,2.在本校再换老板,3.拿硕士走人。当时学校不是特殊
情况是不让博士生转硕士的。和老公商量后选了三。主要有以下几点考虑:1.实在不想
再换老板了,一个博士三个老板三个课题,没法想像。2.当时硕士就业情况不错,如果
拿博士的话,只有一条路,就是薄后。考虑到如果和老公两同时做薄后压力会很大,再
说那时候真的很想要孩子,而且还想要两。考虑再三做了拿硕士走人的决定。当时我父
母不是太赞成,学校committee 也找我谈话,最终还是没有动摇。所以边写硕士论文边
找工作。当时有机化学硕士还是挺好找工作的。也许是五年的时间有些优势,找了三个
月,四个on site ,去了三个,拿了个offer,第四个就推了没去。当时的这份工作一干
就是九年,在一个消费不高的城市,工资也从当时的四万出头到了现在的八万。按理说
好不容易熬到现在,家庭工作都稳... 阅读全帖
P********n
发帖数: 372
2
来自主题: Chemistry版 - Job Opportunity--Scientist -Sensing
This is a CONTRACT position and no visa sponsorship. PM I'll refer to the
recruiter.
I would like to bring your attention to a requirement we have open currently
. Please advise if you or any of your colleagues qualify for the following
job in Norwood, MA
This person would be responsible for participating in a laboratory
environment for research and development programs focused on the goal of
developing and optimizing electrochemical and amperometric biosensors.
Responsibilities:
* Support/Co-d... 阅读全帖
x*****g
发帖数: 82
3
来自主题: Chemistry版 - TL审稿转让
谁有时间申完一篇稿子,明天就是deadline了,我忘了拒绝,领域不是特别熟悉。有人
熟悉这个领域,可以给我发信,我给你转过去。
A series of ladder-type graphene ribbon oligomers have been synthesized
through DDQ/acid-mediated oxidative cyclizations. The oligomers
present a bright blue light emission, and the new oligomers were clearly
characterized by NMR and MALDI-TOF。
R****n
发帖数: 708
4
【 以下文字转载自 Biology 讨论区 】
发信人: RinMan (Walk with the Earth Mother.), 信区: Biology
标 题: 做high-throughput metabolomics和proteiomics的Mass Spec选择
发信站: BBS 未名空间站 (Wed Nov 23 11:56:39 2016, 美东)
系里准备进一台,原来有两台Agilent Q-TOF和QQQ.LC是1100系列,机器有点老了,毛
病多。做高通量,那个组合sensitivity和resolution比较好,再一个比较easy
maintainance。多谢!
f********e
发帖数: 2194
5
来自主题: Chemistry版 - Papers help
Can you please help me with the following articles?
[email protected]/* */
Thank you!
Bioanalysis. 2013 May;5(10):1249-67. doi: 10.4155/bio.13.93.
Performance assessment of microflow LC combined with high-resolution MS in
bioanalysis.
Hongxia Wang & Patrick Bennett
http://www.future-science.com/doi/abs/10.4155/bio.13.93?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
Bioanalysis. 2014 Aug;6(16):2159-70. doi: 10.4155/bio.14.115.
Quantitative proteomics using the high... 阅读全帖
B8
发帖数: 92
6
The Department of Environmental Sciences and Engineering at Gillings School
of GlobalPublic Health, University of North Carolina at Chapel Hill has a
postdoc opening. We are looking for a self-motivated person to conduct omics
profiling to understand the interactions between the gut microbiome and
diseases/environmental exposures. Our lab has dedicated high-end mass
spectrometers, including Q-Exactive HF Orbitrap and 6550 Q-TOF.
A PhD degree or solid training in analytical chemistry or related f... 阅读全帖
t***m
发帖数: 114
7
来自主题: Macromolecules版 - 谁看过这本Springer的新书?
能搞到电子版就好了
Pasch, H., Deutsches Kunststoffinstitut, Darmstadt, Germany;
Schrepp, W., BASF AG, Ludwigshafen, Germany
MALDI-TOF Mass Spectrometry of Synthetic Polymers
2003 XVIII, 298 p. 158 illus. Hardcover
3-540-44259-6
Recommended Retail Price: EUR 79.95 *
http://www.springer.de/cgi/svcat/search_book.pl?isbn=3-540-44259-6¤cy
=Euro&language=English&lastResult=http%3A%2F%2Fwww.springer.de%2Fsearch97cgi
%2Fs97_cgi%3F%26fulltextOperator%3D%253C%2523NEAR%252F4%253E%26sprache%3Dany
%26urheber1%3
s*****s
发帖数: 255
8
来自主题: Macromolecules版 - Anybody here know MALDI-TOF, bruker?
One of my friend uses it and want to know if any free version software can
analysis its output file? Any ideas?
Deeply appreciate!
h***s
发帖数: 111
9
来自主题: Macromolecules版 - 又来问问题 (转载)
MALDI-TOF MS
p******4
发帖数: 38
10
来自主题: Macromolecules版 - one old but classical question about polymer
Chemically, in MS, specially MALDI-TOF MS, you will see two or multiple
series of peaks for polymer blends, but only one series for block copolymers
.
H*****y
发帖数: 9
11
来自主题: Macromolecules版 - one old but classical question about polymer
MALDI-TOF MS 对分子量比较高的polymer也容易分辨吗?

copolymers
z*****7
发帖数: 133
12
来自主题: Macromolecules版 - 求问一个copolymer的问题

GPC能做copolymer么?
MOLDI-TOF怎么样?不过分子量有限制...
m*****e
发帖数: 1506
13
来自主题: Macromolecules版 - NMR vs GPC determine molecular weight?
用GPC with multi-angle laser detector 可测绝对分子量
high-field NMR测核磁分子量,但也有误差
MALDI-TOF也可测绝对分子量,不过不太容易
从转化率可定理论分子量
如果GPC分子量仅relative to polystyrene,NMR分子量应该比较准确
x*****g
发帖数: 82
14
国内有机硕士毕业,博士期间的工作经历如下
Synthesis and characterization of Polymer & monomer, Modification functional
molecules to polymer scaffolds
The application of synthetic biocompatible polymers, like drug and gene
delivery
Operating Instrumentals, NMR, HPLC, GPC, MS (MALDI-TOF),TEM, Confocals IR,
DLS etc.
publication现在只有一篇jacs和国内时候发的几篇有机方向文章,还有4-5篇已经投出
或者即将投出。
明年五月份毕业,不知道能不能在毕业前找到位子。
将来何去何从还没有打算。
请大家给点建议,现在心里很忐忑,谢谢
n**********n
发帖数: 1
15
来自主题: Macromolecules版 - Position open
Hi,
My group has a Senior Research Chemist opening. The person will be
conducting hands-on daily synthesis work as well as supervising a lab
technician for another small project in an industry environment. Below is a
brief summary of the qualifications. Pls. contact me for more details.
Basic:
- PhD/MS in Polymer Science/Organic Synthesis Chemistry
- 2+ (for PhD) or 5+ years (for MS) of work experience in polymer/small
molecule synthesis
- Hands-on experience in instrumental characterizations of... 阅读全帖
z***3
发帖数: 3
16
来自主题: Macromolecules版 - 求审稿 高分子合成方向
求审稿机会。我的方向有环状和星状等不同拓普结构的高分子合成,两性高分子的合成
以及在极性与非极性溶剂中的自组装和药物载释,利用MALDI-TOF MS表征高分子的端基
官能团。有在ACS macro letter, polymer,analytica chimica acta​等杂志上
发表一作6篇,合作文章13篇。 以前帮老板审过JACS,Macromolecules, ACS macro
letter, Polymer等杂志的稿子, 有一定审稿经验。为了将来申请绿卡,现在想开始攒
审稿。如果有朋友可以推荐审稿机会,请发站内信给我,我会提供详细的背景材料和简
历,非常感谢。​
z***3
发帖数: 3
17
来自主题: Macromolecules版 - 求审稿 高分子合成方向
求审稿机会。我的方向有环状和星状等不同拓普结构的高分子合成,两性高分子的合成
以及在极性与非极性溶剂中的自组装和药物载释,利用MALDI-TOF MS表征高分子的端基
官能团。有在ACS macro letter, polymer,analytica chimica acta​等杂志上
发表一作6篇,合作文章13篇。 以前帮老板审过JACS,Macromolecules, ACS macro
letter, Polymer等杂志的稿子, 有一定审稿经验。为了将来申请绿卡,现在想开始攒
审稿。如果有朋友可以推荐审稿机会,请发站内信给我,我会提供详细的背景材料和简
历,非常感谢。​

发帖数: 1
18
本人多年从事polymer chemistry, organometallic, nanomaterials的研究工作,最近
在展审稿数量申绿,求推荐。以下是我的研究领域:
• Strong proficiency in design multi-step organic/organometallic/
inorganic syntheses under the various conditions, design of synthetic
strategy based on goal compound, cultivation of single crystals, synthesis
and characterization of organometallic compounds (Pd, Al, Sc, Y, Ti, Zr, Hf,
Fe, Co, Ni, Cr, Ir, Ru), macromolecules and nanomaterials:
1. Cross-Coupling polymerization: design, synthesis an... 阅读全帖

发帖数: 1
19
本人多年从事polymer chemistry, organometallic, nanomaterials的研究工作,最近
在展审稿数量申绿,求推荐。以下是我的研究领域:
• Strong proficiency in design multi-step organic/organometallic/
inorganic syntheses under the various conditions, design of synthetic
strategy based on goal compound, cultivation of single crystals, synthesis
and characterization of organometallic compounds (Pd, Al, Sc, Y, Ti, Zr, Hf,
Fe, Co, Ni, Cr, Ir, Ru), macromolecules and nanomaterials:
1. Cross-Coupling polymerization: design, synthesis an... 阅读全帖
l******6
发帖数: 11
20
本人现为哈佛大学博士后,主要领域为纳米生物芯片(Nano biosensor)开发。涉及
Nano biosensor design and Nano-fabrication, biomarker detection, single
particle interaction, optical and confocal microscope, optical sensor
development, photonics device/sensor develop. 博士期间为生物学和大分子药物动
力学,涉及proteomics(MALDI-TOF,HPLC-MS), 草本药物有效成分提取,纯化,质
谱分析;细胞辐射及药物干扰蛋白组学研究,鼠,兔等动物药物代谢研究,病理学研究。
以上所述领域均有相关文章发表,一作文章8篇(包括Science Advances,Nano letter
, scientific report, ACS photonics,Optics express,Chemical Research in
Chinese Universities,ACTA CHIMICAL ... 阅读全帖
l*****2
发帖数: 5
21
来自主题: Pharmaceutical版 - 有人愿意谈谈代谢组学?
试一试HILIC?Lipid 会先出来。 看看这个:
http://www.nestgrp.com/pdf/Pp1/HILIC_metab.pdf#search=%22metabolomics%20%20HILIC%22
UPLC当然更好了,不过据说出峰很快, 你的MS 是TOF 还是 ion-trap? 老式ion-trap的
response恐怕是太慢了。
l*******0
发帖数: 12
22
Look for cancer biomarkers, by MALTI-TOF-MS or LC-MS
Any comments about this? Many thanks.
f*******r
发帖数: 78
23
来自主题: Pharmaceutical版 - protein therapeutics前景怎样?
如果真要做好formulation,用到的analytical 也不会少吧,HPLC, FPLC, LC-MS,
MALDI-TOF,
light scattering, UV/Vis, CD spectra, SDS-PAGE, IEF,DSC, activity assay...
感觉或
多或少都会用到,当然对仪器的理解还是比不上纯分析出身的
c******m
发帖数: 884
24
多谢回复,
QTrap上限好像是2800,的确是只能做小肽了。
业界是不是都用Maldi-tof?
b*******g
发帖数: 1309
25
一个orbitrap 可以买两台 Qtrap了吧
lz 还是应该上Q-TOF 比较靠谱
定性定量都能兼顾到,当然有钱还是直接上Orbitrap
f********e
发帖数: 2194
26
q-tof做蛋白也不错
c******m
发帖数: 884
27
多谢各位大侠。
看来我还是到别的实验室去借Q-Tof用吧,虽然有点远。。。
K******S
发帖数: 10109
28
看什么ORBITRAP,什么Q-TOF了,两个的价钱范围有很大的交集
R*******1
发帖数: 6
29
Ad Description for BS/MS Sr Associate Scientist
Responsibilities:
• Work within the Analytical Protein Chemistry area to provide
analytical support for novel peptide and protein therapeutics in pre-
clinical and clinical development.
• Primary responsibilities include the identification and
characterization of post-translational modifications in protein therapeutic
candidates, using state of the art separations technology and mass
spectrometry instrumentation, with an emphasis ... 阅读全帖
b**********s
发帖数: 435
30
本人PhD最后一年,在加州湾区,主要是药物化学合成方面的,正在找暑假实习,路过的童
鞋们帮帮忙,不胜感激!
下面是简单的介绍:
PROFESSIONAL PROFILE
 In-depth and expert hands-on knowledge of organic and medicinal
chemistry, excellent troubleshooting skills, great ability of working
independently and cooperatively;
 Using synthetic and medicinal chemistry methods to develop novel
DNA cross-linking agents targeting on DNA damage repair pathway;
 Expertise in organic compound purification and characterization by
column chromatography, H... 阅读全帖
m*********3
发帖数: 1425
31
来自主题: Pharmaceutical版 - 请问这种面试问题怎么回答?
找LC-MS方面的工作,已经是第二次在电面被同一个问题难住了,对方问我会不会
waters的UPLC和Q-TOF,第一次我回答没用过,但我学起来很快,结果被pass,第二次我
回答虽然没用过,但我有其他HPLC和质谱的经验,而且对方要求的蛋白分析我比较熟悉
,自己学习能力也很强,掌握新机器应该会很快,还是被pass。真不知道以后遇到这种
问题该怎么回答,虽然我是做LC-MS,但我不可能每种机器,每个厂家的都用过,请问面
试遇到类似的问题应该怎么回答?谢谢了。
t**n
发帖数: 4365
32
来自主题: Pharmaceutical版 - 请问这种面试问题怎么回答?
Waters的UPLC没有用过,有点说不过去。。。HPLC和UPLC还是有很多不同。如果用过其
它公司的UHPLC,这时候应该强调一下。
Q-TOF没有用过,MassLynx有没有用过?实在不行,去Waters参观培训一下。
m*********3
发帖数: 1425
33
来自主题: Pharmaceutical版 - 请问这种面试问题怎么回答?
we don't have Waters' UPLC in the lab. I do use Agilent UPLC. I didn't use Q
-TOF and not familiar with Masslynx. I use LTQ-Orbitrap for protein
characterization.
Is Waters培训 enough for interview and routine job?Thanks
m*********3
发帖数: 1425
34
来自主题: Pharmaceutical版 - 请问这种面试问题怎么回答?
I believe so because the hiring manager tells me they only have Waters UPLC
and Q-TOF and they need someone to run it. Maybe some other candidates know
how to run it.

you
and
have

发帖数: 1
35
各位前辈好,
本人今年博士第六年,正面临着毕业找工作。博士期间的工作主要有两个方面,1.
using phage display library (peptide) to screen and identify tumor specific
antigen ligands.
2. development of novel siRNA delivery system.
掌握的技术有
1. Screening and identification of targeting ligands using phage display
2. Culture and propagation of transformed cell lines and primary cell lines(
HUVEC) 3. Cellular biology related techniques: MTT and SRB assay, siRNA
transfection, ELISA, Western blot, RT-PCR, Real time PCR, flow cytometry,
Gene cloning... 阅读全帖
l******6
发帖数: 11
36
本人现为哈佛大学博士后,主要领域为纳米生物芯片(Nano biosensor)开发。涉及
Nano biosensor design and Nano-fabrication, biomarker detection, single
particle interaction, optical and confocal microscope, optical sensor
development, photonics device/sensor develop. 博士期间为生物学和大分子药物动
力学,涉及proteomics(MALDI-TOF,HPLC-MS), 草本药物有效成分提取,纯化,质
谱分析;细胞辐射及药物干扰蛋白组学研究,鼠,兔等动物药物代谢研究,病理学研究。
以上所述领域均有相关文章发表,一作文章8篇(包括Science Advances,Nano letter
, scientific report, ACS photonics,Optics express,Chemical Research in
Chinese Universities,ACTA CHIMICAL ... 阅读全帖
q*d
发帖数: 22178
37
来自主题: Physics版 - 一个可以让你成名的物理实验
这好像不是个啥很有意思的问题,至少PDG给出数据中子的质量和质子质量
几乎差不多一样准确.
一个naively构造出的测量中子质量方案(不清楚有人做过没有):
对热中子用干涉法测其波长,从而动量p=h_b/λ,
中子速度v由TOF给出,从而m=p/v

,实际上有些基本实验还没人做过,比如中子质量的直接测试。
理论上的质子+电子得出,实际上还未被直接测量过。如果有能开发直接测量中子的"原
子称",起码可以一篇Science吧,就这么简单。
p****y
发帖数: 23737
38
来自主题: _Vegetarianism版 - Bill Clinton's Life As A Vegan
http://www.npr.org/blogs/health/2011/08/20/139782972/bill-clint
-vegan?ps=sh_stcathdl
by Nancy Shute
August 20, 2011
Bill Clinton became renowned on the campaign trail for his ability to snarf
up burgers and fries. Heart bypass surgery convinced him to cut back on the
grease. In the past year, Clinton's gone even further: He's gone vegan.
Shots would have pegged the 42nd president as the guy least likely to Go Tof
u. But in an interview with CNN's Sanjay Gupta, the former president talks a
bout ... 阅读全帖
y***k
发帖数: 1078
39
来自主题: _Stockcafeteria版 - GAME seems bottom out
Anyone plays GAME? It seems in a good shape. Today it is added in
nasdaq china index. It is a short term boost.
ZT a post in yahoo message board:
http://messages.finance.yahoo.com/Stocks_(A_to_Z)/Stocks_S/threadview?
m=tm&bn=99506&tid=3657&mid=3657&tof=2&frt=2
GAME's forward P/E is 7, which makes this stock ridiculously cheap
because its growth story is still intact--as demonstrated by the
institutional ownership rising from 29.5 to over 32% since Friday.
GAME's revenues are from the online ma
b*****h
发帖数: 3386
40
来自主题: _pennystock版 - 关于UNG的很好分析
【 以下文字转载自 Stock 讨论区 】
发信人: wagmal (超级偶像,工藤静香), 信区: Stock
标 题: 关于UNG的很好分析
发信站: BBS 未名空间站 (Fri Jun 12 19:42:01 2009, 美东)
意思就是,如果你想long 天然气,不要买UNG,而是买天然气生产企业的股票,你想浪
石油,不要买USO,而是买XLE,如果想浪农产品,不要买DBA,买MOO。
http://messages.finance.yahoo.com/Stocks_%28A_to_Z%29/Stocks_U/threadview?m=te&bn=51477&tid=34061&mid=34061&tof=4&frt=4#34061
UNG Roll 11-Jun-09 05:36 pm
I am going to try to explain objectivly to those that are confused.
If you own UNG you do not own Natural Gas per se. You own a share in... 阅读全帖
n**********6
发帖数: 558
41
来自主题: _pennystock版 - AGM
下周出Q2 ER,具体时间还没看到有权威的公告,搞不懂。我从$5拿着至今,大家还敢
追否?
整体来说上半年美国风调雨顺,Q2肯定比Q1好,再帖上yahoo message board上的一位
哥们的分析:http://messages.finance.yahoo.com/Stocks_%28A_to_Z%29/Stocks_F/threadview?m=tm&bn=27633&tid=5490&mid=5490&tof=13&frt=2
What is a proper valuation for AGM ? 29-Jul-09 11:30 am As I've
posted before, historically AGM has recieved a 10-12 PE, so why not now ?
There is really no reason at all except for the fact that skeptics likely
think AGM will start making substantially less in earnings ... 阅读全帖
s*********8
发帖数: 28
42
来自主题: _pennystock版 - Forward: Picks and Watchlist 8/25/2009
Forward this from other boards, just for your reference.
Picks:
MMGP Closed Monday at .0005 up 66% on over 33 million volume. A/S 490,000,
000 O/S 449,999,999 as reported 3/31/2009. Look for this to run past .001
MTPR Closed even at .0038 after HOD .0049. Float 11,591,404. Look for this
to begin running. Operating company announced that financials are being
prepared for submission. Also working toward uplisting. Had $2.16 million
revenue quarter ending 3/31/2009, increase of 67%.
RDXM Closed Mon... 阅读全帖
b*****h
发帖数: 3386
43
来自主题: _pennystock版 - 讨论一下BPSG的FA吧
FA参数不错,不过不会是空心大萝卜吧?
刚才去yahoo看到的一个讨论
http://messages.finance.yahoo.com/Stocks_(A_to_Z)/Stocks_B/threadview?
m=te&bn=27860&tid=2359&mid=2359&tof=8&frt=2#2359
c***m
发帖数: 2630
44
来自主题: _pennystock版 - Just checked my bad positions recently
http://messages.finance.yahoo.com/Stocks_%28A_to_Z%29/Stocks_X/threadview?m=te&bn=72398&tid=3162&mid=3162&tof=8&frt=2#3162
keep watching.

funds are shorting China.
holding it, and day-trading it to lower the average cost.
N***i
发帖数: 2063
45
来自主题: _pennystock版 - Events and ERs for week Aug 9-13 (转载)
【 以下文字转载自 Stock 讨论区 】
发信人: ll111 (Stealth), 信区: Stock
标 题: Events and ERs for week Aug 9-13
发信站: BBS 未名空间站 (Sat Aug 7 22:26:20 2010, 美东)
Monday, Aug. 9
Scheduled for around noon: President Obama to deliver remarks at University
of Texas in Austin.
No data scheduled.
Tuesday, Aug. 10
House returns for one day to vote on state aid bill.
9 a.m.: Federal Reserve's Federal Open Market Committee meets on interest
rate and monetary policy. Statement to be released at 2:15 p.m., at Fed
headquarters.
8... 阅读全帖
f**********g
发帖数: 2252
46
那斯达克证券市场 - NASDAQ (2011/01/27)
时间 代号 公司名称 涨/跌 最近价格 交易量 涨跌%
13:20 HAFC HANMI FINL CP/汉米金融公司 0.34 1.51 14399188 29.06 %
13:16 BSET BASSETT FURNITR/Bassett家俱 1.3 5.85 167094 28.57 %
12:56 BERK BERKSHIRE BCP/Berkshire 能源资源公司 1.54 7.9 112910 24.21 %
13:15 HMNF HMN FINL INC/HMN财政公司 0.48 3.12 2100 18.18 %
13:19 ACAT ARCTIC CAT INC/Arctic Cat公司 2.38 16.1 356996 17.35 %
13:20 CCME CHINA MEDIAEXPR/ 2.89 22.45 4167913 14.77 %
13:21 NFLX NETFLIX INC/ 26.6 209.63 138541... 阅读全帖
f**********g
发帖数: 2252
47
那斯达克证券市场 - NASDAQ (2011/01/27)
时间 代号 公司名称 涨/跌 最近价格 交易量 涨跌%
13:20 HAFC HANMI FINL CP/汉米金融公司 0.34 1.51 14399188 29.06 %
13:16 BSET BASSETT FURNITR/Bassett家俱 1.3 5.85 167094 28.57 %
12:56 BERK BERKSHIRE BCP/Berkshire 能源资源公司 1.54 7.9 112910 24.21 %
13:15 HMNF HMN FINL INC/HMN财政公司 0.48 3.12 2100 18.18 %
13:19 ACAT ARCTIC CAT INC/Arctic Cat公司 2.38 16.1 356996 17.35 %
13:20 CCME CHINA MEDIAEXPR/ 2.89 22.45 4167913 14.77 %
13:21 NFLX NETFLIX INC/ 26.6 209.63 138541... 阅读全帖
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