y****w 发帖数: 3747 | 1 感觉是个管理问题而不是技术问题,这是项目architect的事儿啊? 说白了还是没太明
白你到底痛苦在哪儿了,你的环境是麻烦,如果你能具体说说现在是什么样子,你想做
什么做不了之类就好多了。
你前面好像说到用动态语句之类,我没搞清楚这个的需求。动态语句多很多灵活性,比
如可以让schema也变化起来;但一般代价sp代码的可读性。
本来用db来做data cleansing听着挺好,问题是很多
special cases,搞得很多计算结果都有问题,然后又得去找query,debug...然后像
query这样的script一样的东西一多,就很难管理。5,6个developer,成百上千的
queries,就像一场灾难...
有没有可能在数据库里用更generic的设计,使得business logic集中管理,debug/
tracing calculation(有点像excel的tracing dependent)更方便?还是说这样的应
用放在application里会更好? |
|
M*****r 发帖数: 1536 | 2 请问如何得到如下的trace/log:
submit a query against a database (not oracle), the return contains some
junk characters.
OBIEE server is on linux and on the same linux machine, using the odbc
driver used by OBIEE, I can get the correct return in other tools.
那怎么能trace/log到OBIEE读入的数据那? |
|
w**2 发帖数: 724 | 3 请问@6是什么意思?
我用ssms打开database看了,一切正常。
求救,大谢!
Unhandled Exception: System.Data.Entity.Infrastructure.DbUpdateException: An
error occurred while up
dating the entries. See the inner exception for details. ---> System.Data.
UpdateException: An error
occurred while updating the entries. See the inner exception for details. --
-> System.Data.SqlClient
.SqlException: The incoming tabular data stream (TDS) remote procedure call
(RPC) protocol stream is
incorrect. Parameter 9 ("@6"): The supplied value is... 阅读全帖 |
|
n*****n 发帖数: 74 | 4 Dear all,
I like a software, QuoteTracker, very much. Especially the feature of tracing
real-time data quotes from Ameritrade streamer. Since I am customer of
Ameritrade, this will save my money for data feeding. So I am pretty
interested in the techniques that can log on to my account and tracing
information and send order by a nice program.
I am not a CS man, so I have no idea about Client-side application
development. I do have a Python script which can retrieve data from
finance.yahoo.com, b |
|
s*******s 发帖数: 132 | 5 不知道这里有没有RR的人,嘿嘿
RR这几天上水木、新浪都特别慢,几乎没法用
Tracing route to newsmth.net [182.50.1.20]
over a maximum of 30 hops:
1 6 ms 4 ms 3 ms 192.168.10.1
2 13 ms 11 ms 28 ms 10.238.128.1
3 14 ms 11 ms 14 ms gig5-1-4.ithcnycy-rtr004.cny.northeast.rr.
com [2
4.24.16.68]
4 14 ms 12 ms 14 ms ae116.esyrnydr-rtr005.cny.northeast.rr.com [
24.5
8.149.52]
5 30 ms 14 ms 16 ms ae2-0.esyrnyaw-rtr001.cny.northeast.rr.com [
24.5
8.149.4]
6 22 ms 26 ms ... 阅读全帖 |
|
z**r 发帖数: 17771 | 6 NH应该是far end PE loopback,这个肯定是从IGP得到,同时也应该在LFIB里,你不激
活ipv4 neighbor,同时看一下show mpls for?
刚才俺也做了个实验,果然不需要在add ipv4下激活ipv4 neighbor,只需要在add
ipv6下激活ipv4 neighbor就可以送ipv6扩展了。
R1----R2----R3----R4
R1 and R4 are dual stack, R2 and R3 are IPv4 only, LDP enabled on all
interface
相关配置如下
!
!
!
ip cef
no ip domain lookup
ipv6 unicast-routing
ipv6 cef
!
!
interface Loopback0
ip address 10.1.1.1 255.255.255.255
!
interface Loopback1
no ip address
ipv6 address 2001::1/128
!
interface Ethernet0/0
no ip address
... 阅读全帖 |
|
x*********n 发帖数: 28013 | 7 听到北京的声音好亲切啊。
哈哈哈。
顿时爱心泛滥。
前面2个客服也比较nice,
然后呢,
说到traceroute的时候,客服说这个time是incremental的,但是我说是each hop的,
后来跑来一个女的又说是incremental的,粗鲁了一下。呵呵。
我觉得吧,虽然是个简单问题,但是这个time是怎么算出来的呢?TTL只能算多少跳吧
,每一跳的时间怎么算呢?好奇啊。
C:\Users\>tracert google.com.hk
Tracing route to google.com.hk [74.125.225.176]
over a maximum of 30 hops:
1 2 ms 1 ms 1 ms 208.86.100.33
2 1 ms 1 ms 1 ms 208.86.100.225
3 3 ms 2 ms 2 ms 74.119.154.2
Trace complete. |
|
m*********1 发帖数: 204 | 8 对的,那一跳都是***的就是那一跳的返回路由没有,或者别的原因。但是并不是丢包
,如果是丢包,这个trace route就结束了。trace route 和ICMP一样的协议 |
|
z****e 发帖数: 3810 | 9 你加这句trace(percentage);
看看trace的结果 |
|
s*******h 发帖数: 2148 | 10 新手土问题,请莫见笑。AS2。
swf文件3M多,所以加了一个preloader,可是load页面以后,还是空白半天,然后
preloader出现,哗的一下就100%了。trace的结果是一开始什么都trace不到,等
显示的时候就已经快3M了。
研究了一下,是不是这个getbytesloaded和getbytestotal都是指时间轴上用到的
assets啊?因为这个东西本来是作为kiosk的,没打算网上用,所以有很多个小mc
放在library里,点了按钮以后再去load。
有没有什么办法让它早点显示进度条? |
|
f*******w 发帖数: 407 | 11 我是刚接触actionscript没到一个星期的新手,请版上的高手指教:
I use following flash actionscript code, got from online, to load the "
Loading.txt" file:
var myTextLoader:URLLoader = new URLLoader();
myTextLoader.addEventListener(Event.COMPLETE, onLoaded);
function onLoaded(e:Event):void {
var myArrayOfLines:Array = e.target.data.split(/\n/,",");
}
myTextLoader.load(new URLRequest("Loading.txt"));
In "Loading.txt", the data of object in a row is (Dim1 Dim2 Dim3 OX OY OZ)
is:
60,12,9,-50,-50,50
84,12,9, 50, 50,50
Then... 阅读全帖 |
|
S*A 发帖数: 7142 | 12
Linux B 家 wifi 的 b43 driver 啊。你以为那个 b43 是基于 broadcomm
提供的文档开发出来的用在比较新的wifi芯片啊? B 家 MIPS 驱动有两个选择,
b43 是自己反向工程出来的,有源码。wl 是 B 家提供的,没源码,
只支持 2.4 MIPS kernel。 Openwrt 如果选 2.6 kernel,那就是用 b43
驱动。还有其他家用 B43 的我就不一一列了。
我只是想指出,很多人捧 B 家的 wifi 芯片,高大上,其实用起来不是那么好。
你说 Asus 用 B家的芯片 firmware 也高大上。如何请问你如何解释隔壁贴子好
几个 id 说那个 Asus 的旗舰路由经常抽风呢?这个不太符合稳定的前提啊。
我再给你一个 binary wl driver 不稳定的示例。
这是用 MBA 和 B 家官方最新的 wl driver (binary) 编译出来的。FC20
最新的 kernel. 经常抽风,我实在没法赞这个 B 家 wifi 芯片高大上。
[289269.848254] Modules linked in: wl(PO... 阅读全帖 |
|
S*A 发帖数: 7142 | 13 对于哪个芯片组更高大上,仁者见仁吧。
我不是很 fan broadcomm 的芯片,尤其是使用 wl driver 的。
先不说没有 open source 的驱动,Broadcomm 连自己的芯片驱动都
写不好,我用 broadcomm 官方的最新的驱动都反复出 kernel stack dump。
需要经常 rmmod/modprobe 和 reboot。
相比之下我用 Atheros 的芯片没有碰到什么问题,还有完整的
开源驱动。
[154474.425321] Hardware name: Apple Inc. MacBookAir6,2/Mac-7DF21CB3ED6977E5
, BIOS MBA61.88Z.0099.B01.1307121317 07/12/2013
[154474.425322] 0000000000000000 000000003e2a4b3c ffff88008ca23dc8
ffffffff81707091
[154474.425325] 0000000000000000 ffff88008ca23e00 ffffffff8108d0ad
f... 阅读全帖 |
|
d*****e 发帖数: 491 | 14 用的switchVPN
是不是这个vpn被服务器block了?
谢谢
Tracing route to 37.61.54.158 over a maximum of 30 hops
1 244 ms 245 ms 244 ms 192.168.0.69
2 245 ms 245 ms 244 ms switchus.switchvpn.com [69.175.89.233]
3 244 ms 244 ms 244 ms dr6506b.ord03.singlehop.net [69.175.1.241]
4 245 ms 254 ms 259 ms xe-1-0-1.ar1.ord6.us.nlayer.net [69.31.111.
29]
5 294 ms 245 ms 245 ms ae5-30g.cr1.ord1.us.nlayer.net [69.31.110.
249]
6 xe-0.equinix.chcgil09.us.bb.gin.ntt.net [206.223.119.... 阅读全帖 |
|
f***y 发帖数: 98 | 15 My build failed with the following error message:
The system is out of resources.
Consult the following stack trace for details.
java.lang.OutOfMemoryError
<>
It happened when jspc just started:
debug="on" deprecation="on" optimize="off">
I had set the en |
|
n*****n 发帖数: 74 | 16 Dear all,
I like a software, QuoteTracker, very much. Especially the feature of tracing
real-time data quotes from Ameritrade streamer. Since I am customer of
Ameritrade, this will save my money for data feeding. So I am pretty
interested in the techniques that can log on to my account and tracing
information and send order by a nice program.
I am not a CS man, so I have no idea about Client-side application
development. I do have a Python script which can retrieve data from
finance.yahoo.com, b |
|
l*0 发帖数: 195 | 17 $ fqterm
[info 0 fqterm_trace.cpp: 44, setMaxTraceLevel] The upper bound of maximum
trace level is 1
[info 0 fqterm_trace.cpp: 46, setMaxTraceLevel] The maximum trace level is
set to 1
Segmentation fault
$
有人遇到么?怎么解决?
fqterm 版本是
""fqterm" 0.9.6.8-1 source package in The Maverick Meerkat"
See
https://launchpad.net/ubuntu/maverick/+package/fqterm |
|
a*******e 发帖数: 3021 | 18 【 以下文字转载自 PhotoGear 讨论区 】
发信人: autoclave (五花肉), 信区: PhotoGear
标 题: 网络时快时慢,是我的硬件问题还是comcast的问题
发信站: BBS 未名空间站 (Fri Dec 30 12:53:32 2016, 美东)
从上个星期开始网络时快时慢,
用google的网络测速器测速有时候正常8Mbps,有时候0.5Mbps,有时候0.1Mbps。打开网
页也有时候打不开,但F5一下又可以了。我觉得可能是comcast最近出问题了,但是联
想到最近it给我好几个留言让我升级cablemodem(是dosis2.0),不知道会不会是我的
modem的问题,但是以前一直用的好好的好几年了阿。
童鞋们看看下面的tracert和ping老丢包, 是comcast的问题还是我的问题?
tracert -d 208.67.220.220
Tracing route to 208.67.220.220 over a maximum of 30 hops
1 9 ms 8 ms 7 ms 192.168.1.1
... 阅读全帖 |
|
t****t 发帖数: 6806 | 19 not sure about automatic tracing. and i think the yacc itself doesn't have
the solution. you can use valgrind on the generated program, though.
about yydebug, refer to "info bison", section "Tracing". there's an example
there.
60 |
|
w****h 发帖数: 212 | 20 我不知道怎么在debugger中trace,只会在delphi里trace
但这个问题我已经解决了,多谢 |
|
c****e 发帖数: 1453 | 21 那么多讨论debugging的帖子都没有提到windbg的.难道大家在windows上debug都是VS?
windows和gdb相比较的是windbg,超级强大. VS完全是另外一个路子的, 虽然code base
开始是一样的. 当然manage code debugger那一套还是VS方便. windbg超多extension,
一点不比gdb弱. tracing还有基于trace的checker windows也有很多方案, 不比
valgrind差, 不光是memory leak, registry, file, concurrency都可以check. |
|
c****e 发帖数: 1453 | 22 en.可能我没说清楚.windbg就是debugger.valgrind是个emulator.valgrind有个缺点是
纯粹runtime的,不能take a trace然后在trace file上做。我还是两年前用过,不知
道现在怎么样。
发信人: thrust (祝阳阳早日康复), 信区: Programming
标 题: Re: Re: 编程语言选择问题
发信站: BBS 未名空间站 (Thu Jan 8 03:01:27 2009), 转信
我相信windbg走的和valgrind不是同一个路子, 功能也不能相比
valgrind基本上是个模拟器, boundschecker还比较接近
可能因为我做硬件, 对模拟器比较熟-_- |
|
c****e 发帖数: 1453 | 23 基本的monitor看起来简单,但是大系统还是自己开发的居多,配些Agent读counter只
是很小的一部分工作。其实基本的counter,log/tracing,alert这些还不是很麻烦。麻
烦的是diagnostic,这个也是monitoring绑在一起的。
比如Alert来了E2E latency 75th pecential涨了200%, 怎么才能快速找到问题?Real
time的log search, end to end tracing, dump analysis这些才是重点。而且数据大
了,快速的aggregation也很有挑战。
再发散一点,发现了问题,快速搞定才是王道。一般来说code to production还是比较
慢的。configuration做的比较好就可以很快的migrate.
回到monitoring把自己搞死,是完全可以想象的。如果self-monitoring做的不好,
monitoring agent本身消耗的资源太多,就会影响service本身的运行。比如有人issue
一些interactive的log query,这些会被break ... 阅读全帖 |
|
n****1 发帖数: 1136 | 24 1. ref count其实只是GC strategy的一种, 而且是非常普遍的一种. 所以zhaoce拿ref
count来说事是很不妥的.
"Tracing and reference counting are uniformly viewed as being fundamentally
different approaches to garbage collection that possess very distinct
performance properties. [...] they are in fact duals of each other. [...]
all high-performance collectors [...] are in fact hybrids of tracing and
reference counting."
2. 内存管理策略有很多种,GC(包括ref count)只是最无脑的一种. 其他的策略往往是
根据具体模型的特点, 在适当的时候高效地申请和释放内存. 内存池就是典型的例子.
java直接rule out这种可能性.
不要老说... 阅读全帖 |
|
T********i 发帖数: 2416 | 25 一般有stack trace就差不多了。
迄今我靠stack trace都搞定了。
其实,如果实现对头。一个大系统,new和delete的数量板手指都数出来。至少我的系
统是这样。 |
|
发帖数: 1 | 26 你提到的這個文件夾平鋪審美我很不適應,什麼雜七雜八的asm, defs, os, signal,
sys都混入了,真正runtime文件也就幾個,放到arch文件夾裡不行麼?要是像gcc那樣
支持幾十種處理器,豈不是有幾百個文件?
HACKING.md os_linux_noauxv.go
Makefile os_linux_ppc64x.go
alg.go os_linux_s390x.go
append_test.go os_nacl.go
asm.s os_nacl_arm.go
asm_386.s os_netbsd.go
asm_amd64.s os_netbsd_386.go
asm_amd64p32.s os_netbsd_amd64.go
asm_arm.s os_netbsd_arm.go
asm_arm64.s os_openbsd.... 阅读全帖 |
|
n*****n 发帖数: 74 | 27 Dear all,
I like a software, QuoteTracker, very much. Especially the feature of tracing
real-time data quotes from Ameritrade streamer. Since I am customer of
Ameritrade, this will save my money for data feeding. So I am pretty
interested in the techniques that can log on to my account and tracing
information and send order by a nice program.
I am not a CS man, so I have no idea about Client-side application
development. I do have a Python script which can retrieve data from
finance.yahoo.com, b |
|
z***y 发帖数: 7151 | 28 实话实说,当初写这个站的人很扎实.代码漏洞很少很少.比jiaoyou8.com 要强很多。
但是补丁还是要经常打。 向那个axis xml parser的DoS补丁。 还有http trace , 最好
disable. 我在apache.org上看到可以用mod_rewrite deny trace request, 可以参考一
下。
en , 还有那个列印目录, 没有必要吧? images 和utils 开那么大的门?
jiaoyou8.com 就是一个马蜂窝乐。是不是同一个人写的很难说。cross site scripting
漏洞比比皆是。还是一个收费的网站。en , ft yah ft. |
|
n*o 发帖数: 442 | 29 昨天还用的好好的,现在可以起动,但只要一访问任何站点,
就自动关闭netscape.
给出信息如下:
ERROR: ld.so.1: netscape: fatal: /users/rtfm5/wu/.netscape/plugins/trace.txt: unknown file type
Cant load plugin /users/rtfm5/wu/.netscape/plugins/trace.txt. Ignored.
[2] Bus error netscape
那位大侠指点一下? |
|
n*****n 发帖数: 74 | 30 Dear all,
I like a software, QuoteTracker, very much. Especially the feature of tracing
real-time data quotes from Ameritrade streamer. Since I am customer of
Ameritrade, this will save my money for data feeding. So I am pretty
interested in the techniques that can log on to my account and tracing
information and send order by a nice program.
I am not a CS man, so I have no idea about Client-side application
development. I do have a Python script which can retrieve data from
finance.yahoo.com, b |
|
t****q 发帖数: 34 | 31 passmaster里的题,A4里关于Audit procedures by transaction Cycles.
我觉得这道题答案是不是错了??
Which of the following procedures would be most appropriate for testing the
completeness assertion as it applies to inventory?
c.Tracing inventory items from the tag listing back to the physical
inventory quantities.
d.Performing cutoff procedures for shipping and receiving.
答案是D。我选的是C
书上明明说test completeness,the auditor should also trace from a sample of
prenumbered inventory tags to the physical inventory report sheets.
... 阅读全帖 |
|
x**********g 发帖数: 146 | 32 The pronouciation is same as trace, not track, I dont know what the word
actually is. 我就回答了我想作health的, 并说了一下原因, 但似乎他问的并不是
这个。所以我就比较好奇, 这个trace到底指什么。 |
|
s******o 发帖数: 298 | 33 Researchers begin to trace the ancestry of massive galaxies.
by Elesa Janke
By studying quasars and their host galaxies with the Hubble Space Telescope
(HST), a team of astronomers has begun to trace the “family tree” of giant
elliptical galaxies. The group's findings reveal that 10 billion years ago,
when the universe was one third of its present age, these galaxies were
already forming, converting large amounts of gas into luminous families of
stars.
Among the most luminous objects ever found, |
|
s*****t 发帖数: 1994 | 34 11 Hour Star Trails
Credit & Copyright: Josch Hambsch
Explanation: Fix your camera to a tripod, lock the shutter open, and you can
make an image of star trails - graceful concentric arcs traced by the stars
as planet Earth rotates on its axis. Of course, the length of the star
trails will depend on the exposure time. While exposures lasting just five
minutes produce a significant arc, in about 12 hours a given star would
trace out half a circle. But in any long exposure, the background glow from |
|
S******G 发帖数: 965 | 35 没有磷也能繁殖is an overstatement。
之多他们可以说,trace amount的磷 + 很多砷可以sustain, 至于需不需要这trace
amount的磷,那还很难说咧。 |
|
s********n 发帖数: 2939 | 36 trans是相对于cis而言的,指的是fatty acid里的double bonds的configuration,真
正完全氢化的脂肪是没有trans fat的,只是有些部分氢化的才有。这里是wiki的解释:
Trans fat is the common name for unsaturated fat with trans-isomer (E-isomer
) fatty acid(s). Because the term refers to the configuration of a double
carbon-carbon bond, trans fats may be monounsaturated or polyunsaturated but
never saturated.
Unsaturated fat is a fat molecule containing one or more double bonds
between the carbon atoms. Since the carbons are double-bonded to each other,
th... 阅读全帖 |
|
v*****s 发帖数: 20290 | 37 我知道的这个方法就是测分子在溶液里面的diffusion constant,还是make sense的。
你这么想两个极端情况,分子完全不动,那你的time trace要么是0,要么是1,反正是
个constant,time correlation是1,分子动得特别快,time trace都是从0一下子跳到
1,或者从1一下子跳到0,time correlation是0。其他时候介于两者之间,分子扩散越
快,time correlation越小。其中还有一个参数是分子体积,如果分子体积已知,就可
以算diffusion constant,反过来如果diffusion constant已知,就可以算分子体积。
不知道怎么测细胞。 |
|
v*****s 发帖数: 20290 | 38 我知道的这个方法就是测分子在溶液里面的diffusion constant,还是make sense的。
你这么想两个极端情况,分子完全不动,那你的time trace要么是0,要么是1,反正是
个constant,time correlation是1,分子动得特别快,time trace都是从0一下子跳到
1,或者从1一下子跳到0,time correlation是0。其他时候介于两者之间,分子扩散越
快,time correlation越小。其中还有一个参数是分子体积,如果分子体积已知,就可
以算diffusion constant,反过来如果diffusion constant已知,就可以算分子体积。
不知道怎么测细胞。 |
|
c********r 发帖数: 1125 | 39 不是这个领域的,但是读过一些这个领域的文章,另外主要的3个实验室boss的seminar
听过2个,瞎说几句,真正内行不要笑话。。。
实际从前两年开始已经有开始质疑lgr5+cell 是isc细胞的了,比如这篇文章。
Nature. 2011 Sep 18;478(7368):255-9.
A reserve stem cell population in small intestine renders Lgr5-positive
cells dispensable.
这篇ncb是hans clevers实验室也就是最早发现lgr5并且建立整个story体系的实验室,
以前他们还做出了非常fancy的lgr5+细胞的单细胞体外培养出有功能的器官(小肠,
胃。。。)
但是越来越多的证据显然显示lgr5不是唯一的决定性因子,这篇cell的主要卖点觉得就
是这个,所以lrig1+与lgr5+的细胞到底是不是一群实际上是非常关键的问题。。。
如果cell的data是确实的话那么lgr5的重要性显然要大打折扣,但也不至于完全颠覆,
毕竟clevers实验室的体外单细胞培养器官再造和动物移植模型已... 阅读全帖 |
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n******7 发帖数: 12463 | 40 Resisting Arsenic
The discovery of a bacterium living in the extreme conditions of Mono Lake,
California, created a major controversy because it was claimed to be able to
grow solely on arsenic and could substitute arsenate for phosphate in its
key macromolecules, including DNA. Working with the same Halomonas spp.
bacterium, known as GFAJ-1, and ultrapure reagents, Erb et al. (p. 467,
published online 8 July) found that the bacterium needed a low level of
phosphate (1.6 μM) to grow at all. Rath... 阅读全帖 |
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l********k 发帖数: 14844 | 41 A comparison of imaging ray traces that occur between the specimen and
immersion objectives (both oil and water) is presented in Figure 3, along
with a geometric model prediction of an x-z image for a fluorescent bead 100
micrometers below the interface in aqueous media. In Figure 3(a), the
sphere on the left represents the actual shape of the fluorescent bead,
while the elongated shape on the right is the impression provided by imaging
with an oil immersion objective. Ray traces (yellow arrows ... 阅读全帖 |
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l**********1 发帖数: 5204 | 42 PAM or NAM 需要用针对上游和下游的targets 不同的agonist or antagonist
进一步测试的
具体麻烦 参考一综述
Coddou C et al. (2011)
Activation and regulation of purinergic P2X receptor channels.
Pharmacol Rev. 63: 641-83.
PubMed link:
http://www.ncbi.nlm.nih.gov/pubmed/21737531
its contents likes
>The term cooperativity is frequently
used to describe the magnitude of an allosteric
effect, which could be either positive or negative, leading
to up- or down-regulation of receptor function.
and
>A common aspect of P2XRs is their
regu... 阅读全帖 |
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z****u 发帖数: 1007 | 43 对的,很多研究单纯就靠几个所谓marker的表达水平来鉴定stem cell identity确实不
可行。
lineage tracing不完美,比如leakage等问题。不过已经是当前能做到的最好的了。依
然该是金标准。
没有一个marker是完美的。比如alexandra joyner的那篇关于GLi1+ cell作为hair
follicle stem cell marker的文章。单纯从lineage tracing结果来看其实并不完美,
其对hair follicle的contribution并不完全,但是重要的是他们揭示了这群gli1+
cell是受神经分泌的Shh的调控的。这就重要了。marker不是目的,找到一个marker就
意味着一个in vivo model的建立以及一个可能的新的regulatory mechanism.
到底什么是stem cell.我很赞同你的观点。。stem cell 到底是个identity还是个功能
? 这个问题现在很难回答。 |
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z****u 发帖数: 1007 | 44 经典的干细胞理论模式和定义基本都是来源于HSC的研究,单个HSC以一种hierachical
的方式逐级重建整个H-lineage 。也由此self-renewal,multipotential 成为干细胞的
定义。
但实际上别的organ的干细胞并非如此。self-renewal是当然的。可multipotential 这
可真未必了。unipotential的就不能叫stem cell? 我认为这个定义需要修改。实际上
在现在很多stem cell研究里multipotential已不再是评价指标之一了,尤其在lineage
tracing 技术出现之后。有意思的是,好像HSC目前好像没人以lineage tracing的方
式去做过研究吧。我对HSC知道不多.
关于hierachy, 可能只有HSC被了解得很清楚。别的tissue specific stem cell是否也
有这样的hierachy? 我自己感兴趣的是transit amplifying cell (TA cell). 在mouse
incisor里面可以有很明确的TA cell population .可对于别... 阅读全帖 |
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h**i 发帖数: 8 | 46 【 以下文字转载自 Macromolecules 讨论区 】
发信人: hexi (格温普兰), 信区: Macromolecules
标 题: two baozi for how to remove water from polymer
发信站: BBS 未名空间站 (Tue Aug 31 20:06:44 2010, 美东)
if a large scale of polymer has been dissolve in nonpolar solvent and washed
by water, the polymer would be contain small trace of water in it. Anyone
suggest a way to remove the trace of water by a simple method without
bringing any chemical change? Thanks. |
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s******a 发帖数: 220 | 47 我们实验室有以下
GC/MS FOR VOLTAILES
GC/MS FOR SEMIVOLATILES
GC/FIDGC/PID
GC/ECD
ICP/MS-DRCe for trace metals
AA for trace mercury
等等
如果您有项目,本实验愿意提供合作。谢谢各位宝贵的时间!
425-214-5868 |
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s******d 发帖数: 323 | 48 The moisture-density relationship said it's medium brown fine sand with
trace gray fine sand and trace roots. |
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s******a 发帖数: 220 | 49 我们实验室有以下
GC/MS FOR VOLTAILES
GC/MS FOR SEMIVOLATILES
GC/FIDGC/PID
GC/ECD
ICP/MS-DRCe for trace metals
AA for trace mercury
等等
如果您有项目,本实验愿意提供合作。谢谢各位宝贵的时间!
425-214-5868 |
|