g***m
发帖数: 465 | 1 check NEB catalog for Taq enzyme, it has double strand specific 5'->3'
exonuclease activity.
My guess is primer and/or product degradation by Taq.
BTW, one small tip for pfx is: using 2X buffer rather than 1X. I got several
cDNA clones by PCR, and none of them disrupt the ORF by this modification.
DNA(WASP)
然后用同样的primer
colony-pGEX4t1
这些在处理后的pGEX4t1 |
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