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发帖数: 70 | 1 make sure that you are aware of the buffer effect:
It is estimated that DNA has 15% lower absorbance in TE
buffer, 23% lower in TE+saline compared to water.
1 O.D.260 are 38, 45 and 50 ug/ml for DNA in ddH2O,
TE and TES, respectively. I assume the same for RNA,
and 1 O.D.260 are 40 ug/ml for RNA in TE (from Ambion).
btw, A260/A280 is different for pure RNA
DEPC'd H2O (pH5-6) 1.60
Nuclease free H2O (pH6-7) 1.85
TE (pH8.0) 2.14 |
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