g****r
发帖数: 14 | 1 I have been using invitrogen 2 step RT kit for a while,
always give me very good result ...
On possible is in your case, your total RNA might
be partially degraded, so that you can only get the
shorter segment, instead of the 1kb product
suggestions:
1) double check your primer design
and PCR condition (TM, Mg conc. pfx? or taq, or pfu?)
2) if everything seems to be correct
be more strict with your RNA extraction
use the ambion RNaseZap to remove environmental factors
(just as much a |
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