j********o
发帖数: 2 | 1 Yes. Not only BL21codon plus, but almost all BL21s except BL21(DE3)pLysS/E are
leaky. pLysS/E encode enzyme that degrade T7 polymerase to avoid leaky.
In your case with many rare Arg codons, you have to use codon plus. So, it is
importment to avoid leaky? otherwise, just express with leaky if it does no
harm. | j********o
发帖数: 2 | 2 BL21codon+ is indeed BL21 with a plasmid that expresses rare codons and
BL21(DE3)pLysS/E is indeed BL21 with a plasmid that expresses an enzyme that
degrade T7 polymerase to avoid leaky. Both plasmid are chlorepenicol
resistance. In inder to make codon plus in BL21(DE3)pLysS/E strain, drug
marker of one plasmid must be change and the system will become too
complicated - the strain resist to two drug, plus another drug marker for your
recombinant protein.
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