D***a
发帖数: 516 | 1 构建的质粒总有几个碱基测不出来，有时候是单个的，有时候是几个连在一起，应该不
是缺失突变了吧？ |
J****n
发帖数: 156 | |
k*******s
发帖数: 214 | 3 这种情况下要重新测序，如果一定要make sure.
要是测序有困难我帮你测吧。
【在 D***a 的大作中提到】
: 构建的质粒总有几个碱基测不出来，有时候是单个的，有时候是几个连在一起，应该不
: 是缺失突变了吧？
|
h****6
发帖数: 229 | 4 If it can't be sequenced at the location due to 2ndary structure, the
electrograph should have gaps (will show as NNN). If nt is continuous read,
there is deletion. It happens with PCR product. Primer directed gene
synthesis may generate deletion as well |
D***a
发帖数: 516 | 5 是啊，不是N，那我得从头开始做了
,
【在 h****6 的大作中提到】
: If it can't be sequenced at the location due to 2ndary structure, the
: electrograph should have gaps (will show as NNN). If nt is continuous read,
: there is deletion. It happens with PCR product. Primer directed gene
: synthesis may generate deletion as well
|
