j*****q
发帖数: 82 | 1 rt
how to amplify extreme AT-rich sequences on genomic DNA?
followed some literatures to decrease the enlongation temp. to 60 degree,
but still failed.
i am using the roche polymerase and qiagen one.
seeking for helps!
please!!! |
f********n
发帖数: 6465 | 2 这些试验技术问题最好去丁香园问去。
我们这里比较关注人生命运等等抽象的问题。
哈哈哈。对不起啊。玩笑玩笑。 |
h****6
发帖数: 229 | 3 you may try Phusion or Phire. This polymerase has dsDNA binding domain that
helps to stabilize dsDNA. |
j*****q
发帖数: 82 | 4 any suggestion?
i changed many parameters, only got smear.
really frustrated. |
