e**r 发帖数: 1144 | 1 各个公司的GC buffer应该都是差不多的东西吧
关键成分是什么? | m*******n 发帖数: 387 | | a***e 发帖数: 1010 | | e**r 发帖数: 1144 | 4 应该不是吧
因为买的酶包装里DMSO是单独提供的
【在 a***e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 5% DMSO final
| a***e 发帖数: 1010 | | c****1 发帖数: 1095 | | n********k 发帖数: 2818 | 7 google is ur best friend...anyway, in general, it is denature reagent and/or
detergent including DMSO, Betaine, NP40, Triton-X100 etc etc...what I heard
Betaine is likely the best for most of application...and u can buy it from
some company...nonetheless, it could still be tricky even if you know the
gradient, it could still take a hell a lot effort to figure out correct
composition...I personally have used mostly DMSO, and Q solution from qiagen
and a bit betaine...
【在 e**r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 各个公司的GC buffer应该都是差不多的东西吧 : 关键成分是什么?
| n***e 发帖数: 180 | 8 Phusion的话GC buffer有3.5%DMSO,其它的酶有可能是betaine | p******i 发帖数: 1092 | 9 对,没错,我试过PHUSION的GC BUFFER再加 8% 的DMSO…… |
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