m**********3 发帖数: 706 | 1 I am doing a coIP (use a mouse antibody to pull down and use a rabbit
antibody for IB) and found my band
overlap with a rabbit IgG band. Any reccomendation for a good secondary
without IgG band or coIP? |
s******y 发帖数: 28562 | 2 I am a little bit confused.
Why don't you use protein A/G to do the pulldown?
Why do you want to use 2nd ab for pulldown?
【在 m**********3 的大作中提到】![](/moin_static193/solenoid/img/up.png) : I am doing a coIP (use a mouse antibody to pull down and use a rabbit : antibody for IB) and found my band : overlap with a rabbit IgG band. Any reccomendation for a good secondary : without IgG band or coIP?
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d****u 发帖数: 1553 | |
n******d 发帖数: 1055 | 4 stop doing IP. that is the solution. |
s***a 发帖数: 671 | 5 如果你说的是heavy chain band 干扰的话,可以尝试light chain specific的二抗。 |
p******i 发帖数: 1092 | 6 同問
【在 d****u 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 你的膜上为什么会有rabbit IgG?
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