a******n 发帖数: 392 | 1 如果要在细胞系里同时knockdown两个基因,怎么选择RNAi的方法阿?siRNA,
lentiviral shRNA? 效率哪种高? | a***e 发帖数: 1010 | 2 Both siRNA and lentiviral shRNA methods are good enough to reach your
goal.
However, lentivirus usually has a higher infection efficacy than
transfection method. Therefore, using lentiviral shRNA may be a more
efficient
way to knockdown gene expression. | a******n 发帖数: 392 | 3 多谢。如果用lentiviral shRNA,infection 之后几天可以用puromycin测定
transduction的效率? 在加puromycin之
前,需要把细胞重新plate吗?
【在 a***e 的大作中提到】 : Both siRNA and lentiviral shRNA methods are good enough to reach your : goal. : However, lentivirus usually has a higher infection efficacy than : transfection method. Therefore, using lentiviral shRNA may be a more : efficient : way to knockdown gene expression.
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