m******5
发帖数: 1383 | 1 for knock in/ knock out mouse
大家一般用哪种方法纯化linealized 质粒 | j*********o
发帖数: 237 | 2 After linearization, extract the DNA as follows:
-1X Phenol
-1X Phenol:Chloroform
-2X Chloroform
-Precipitate with EtOH and resuspend in water at a final concentration of
about 1g/l | m******5
发帖数: 1383 | 3 Thank you !
I was wondering that why not just use column purification instead?
【在 j*********o 的大作中提到】
: After linearization, extract the DNA as follows:
: -1X Phenol
: -1X Phenol:Chloroform
: -2X Chloroform
: -Precipitate with EtOH and resuspend in water at a final concentration of
: about 1g/l
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