s****a
发帖数: 454 | 1 the antibody will be polyclonal antibody for IP.
Shall I use IgG or serum for preclear? |
s****a
发帖数: 454 | |
m***o
发帖数: 272 | 3 I used rabbit IgG before when my antibody is rabbit. |
w**t
发帖数: 52 | 4 preclear is not essential if your protein of interest is not sticky. Usually
, I spin at high speed for 20-30 min and works well for most of targets.
If preclear is required, you can try to incubate with proteinG beads only
for 1 hr.
I think IgG or pre-immu serum should be used as negative control but not for
preclear. |
n***1
发帖数: 45 | 5 都可以，你只用beads preclear也行，但要有lgG control
【在 s****a 的大作中提到】
: the antibody will be polyclonal antibody for IP.
: Shall I use IgG or serum for preclear?
|
s****a
发帖数: 454 | 6 好,谢谢大家.原来都不知道单用beads也可以preclear |
s****a
发帖数: 454 | 7 恩,我的一个蛋白就是要preclear才好.
Usually
for
【在 w**t 的大作中提到】
: preclear is not essential if your protein of interest is not sticky. Usually
: , I spin at high speed for 20-30 min and works well for most of targets.
: If preclear is required, you can try to incubate with proteinG beads only
: for 1 hr.
: I think IgG or pre-immu serum should be used as negative control but not for
: preclear.
|
