M*******C
发帖数: 183 | 1 同一个DNA样品，根据vector map，T3, T7 两个方向测序，primer都是测序中心提供。
T3的结果正常，T7却NO band, NO data。 再测一次，还是这样。
大家觉得这是怎么回事？thx | b***8
发帖数: 31 | 2 From Promega:
Due to a sequence
difference between the T7 Promoter Primer (Cat.# Q5021) and the T7 RNA
polymerase promoter in the pCI-neo Mammalian Expression Vector, the T7
Promoter Primer cannot be used for sequencing these expression vectors. The
sequence difference is downstream of the transcriptional start site and is
located at the 3′-end of the T7 Promoter Primer. The T7 EEV (Eukaryotic
Expression Vector) Promoter Primer (Cat.# Q6700) has been designed
specifically to prime sequencing reactions from the mammalian expression
vectors, pCI-neo (Cat.# E1841), pSI (Cat.# E1721), pCI (Cat.# E1731) and
pALTER®-MAX (Cat.# Q5761). | M*******C
发帖数: 183 | 3 原来如此！
多谢
The
【在 b***8 的大作中提到】
: From Promega:
: Due to a sequence
: difference between the T7 Promoter Primer (Cat.# Q5021) and the T7 RNA
: polymerase promoter in the pCI-neo Mammalian Expression Vector, the T7
: Promoter Primer cannot be used for sequencing these expression vectors. The
: sequence difference is downstream of the transcriptional start site and is
: located at the 3′-end of the T7 Promoter Primer. The T7 EEV (Eukaryotic
: Expression Vector) Promoter Primer (Cat.# Q6700) has been designed
: specifically to prime sequencing reactions from the mammalian expression
: vectors, pCI-neo (Cat.# E1841), pSI (Cat.# E1721), pCI (Cat.# E1731) and
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