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a***y
发帖数: 19743 | 1 请问，目前，为达到合理的coverage，Drosophila和mouse分别看，一个rep需要多少钱？
RNA-seq
Proteomics sequencing
都要是quantitative的，期望是能取代Microarray
谢谢！ | l**********1
发帖数: 5204 | 2 454 GS FLX+
● Up to 1000bp
● 99.997% accuracy
● 1M reads, 700bp
0.7 Gbase/day
● Homopolymer issue
● Cost ~$14K for run
● $500K instrument
$20K/Gbase
---
or soon later you can try:
Ion Torrent PGM
● Long Reads and Fast
● No optics, CMOS chip sensor
● Uses standard nucleotides
● Up to 300bp reads (going to 400bp)
● >99.5% raw
● >99.999% consensus
● 318 (11M wells) >1Gbase in 3 hours
● 8 Gbase/day possible
● Micron sized well, 22mmx22mm chip
moving to 1 Billion wells per chip
● $700 per run (1 Gbase)
● $50K for the instrument
cited from
HTTPS://www.nescent.org/wg/academy/images/f/f5/Lecture_1_-_Sequencing_
History_and_NGS_Tech.pdf
还有一篇法国INRA 的RNA-Seq 报价比较表
Illumina Genome Analyzer
(Illumina)
www.illumina.com/
(released in June 2006)
~1 TB in raw data,
300 MB in sequence results
~/Mbp
~200-fold cheaper than Sanger
-------------
SOLiD DNA Sequencer
(Applied Biosystems)
www3.appliedbiosystems.com
(released in October 2007)
~2 TB in raw data
~/Mbp
~200-fold cheaper than Sanger
-----------
GS FLX System
(Roche Diagnostics)
www.454.com/
(released in October 2005)
~0/Mbp 0K/Gbp
~50-fold cheaper than Sanger
pdf link URL address can not be got
you can only Google it with below link:
//www.google.co.uk/search?complete/search?
client=hp&hl=en&cp=181&gs_id=2&xhr=t&q=www5.paris.inra.fr%2Fdepe%2Fcontent%2F%20Next-
generation%20high-
throughput%20sequencing%20technologies.%20Technological%20worksheet%20November%202008.%20Cat
herine%20Golstein%20and%20Michel%20Caboche%2C%20Technologies%20&ech=1&psi=8kU5T7uMLuiQ4gS
k9oz1AQ.1329153523062.1&emsg=NCSR&noj=1&ei=8kU5T7uMLuiQ4gSk9oz1AQ
then only one hit is that pdf report.
i.e.
Now You can get outsourcing NGS service from University of Pittsburgh
if you are at PA near PA
//www.genetics.pitt.edu/forms/flyers/GPCL-NGS%20flyer2_11.pdf
//www.ii.uib.no/~inge/inf389-2010/nihms229948.pdf
钱？
【在 a***y 的大作中提到】
: 请问，目前，为达到合理的coverage，Drosophila和mouse分别看，一个rep需要多少钱？
: RNA-seq
: Proteomics sequencing
: 都要是quantitative的，期望是能取代Microarray
: 谢谢！
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