m***o 发帖数: 272 | 1 分析一个gene的promoter,另一个lab帮做DNA的甲基化。发现他们设计的引物不是编码
链的。如果DNA的一个位点methalytion,那么另一条链对应的DNA也一样methalytion
吗。如果这样的话,他们的结果就没错,如果不,那就是要重新设计引物了。引申的问
题就是 TF bind 一条还是double DNA strand?
xiexie! |
m***o 发帖数: 272 | 2 Is it a silly question or nobody know? |
H****N 发帖数: 997 | 3 It does not matter which strand the primer is from. The methylaton on either
strand can always be detected. Most TFs binds to both strand of DNA, but
there are exceptions. |
m***o 发帖数: 272 | 4 Thank you! that is very helpful! You are very smart! |
j****x 发帖数: 1704 | 5 transcriptional factors are Double-strand DNA binding proteins
【在 m***o 的大作中提到】 : Is it a silly question or nobody know?
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