i*****i
发帖数: 154 | 1 最近老板要做RNA-Seq看lncRNA的表达。
Illumina的Kit要做PolyA的selection。
但是有人说有些lncRNA是没有polyA尾巴的，所以我问问版上的大侠，大概有多少
lncRNA是没有polyA尾巴的。
是不是用Ribo-zero 处理sample做library更合适一些？
谢谢 |
i**y
发帖数: 71 | 2 Some working definitions of lincRNA include polyadenylation |
j*p
发帖数: 411 | 3 大部分(>90%)lncRNA有polyA. Ribo-zero 数据远不如polyA selected数据来得平滑.
just a trade off, up 2 you |
p******b
发帖数: 379 | 4 MALAT1好像就没有尾巴， 3‘有个triple helix 结构， |
r*****8
发帖数: 2697 | 5 MALAT1应该有polyA tail, 否则TruSeq应该无法检测到
【在 p******b 的大作中提到】
: MALAT1好像就没有尾巴， 3‘有个triple helix 结构，
|
b******y
发帖数: 627 | 6 Work from Phil Sharp lab showed that MALAT1 is not polyAdenylated. As
pointed out by polycomb, it has a triple helix. One or two strands of the
triple helix is polyA like, which is encoded in the gene. |
r*****8
发帖数: 2697 | 7 有道理, 刚刚看了下Sharp的paper, 对照了RNAseq data, 比较吻合
【在 b******y 的大作中提到】
: Work from Phil Sharp lab showed that MALAT1 is not polyAdenylated. As
: pointed out by polycomb, it has a triple helix. One or two strands of the
: triple helix is polyA like, which is encoded in the gene.
|
