I*******0 发帖数: 602 | 1 如果IgG拉下来的片段扩不出来东西,也就是没有Ct值,请问计算的时候用什么值,随
便给一个比较搞的Ct值吗?比如45? | a****o 发帖数: 1786 | 2 you can use IP efficiency, ie, compared to input, how much DNA was pulled
down. or use some known non-binding sites as control.
No baozi necessary.
【在 I*******0 的大作中提到】 : 如果IgG拉下来的片段扩不出来东西,也就是没有Ct值,请问计算的时候用什么值,随 : 便给一个比较搞的Ct值吗?比如45?
| I*******0 发帖数: 602 | 3 多谢,不过那种负对照的位点经常拉下来的也是0,还是搞不懂如果没有Ct值最后怎么
计算
【在 a****o 的大作中提到】 : you can use IP efficiency, ie, compared to input, how much DNA was pulled : down. or use some known non-binding sites as control. : No baozi necessary.
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